Flea peritrophin nucleic acid molecules, proteins and uses thereof

ABSTRACT

The present invention relates to flea peritrophin proteins; to flea peritrophin nucleic acid molecules, including those that encode such flea peritrophin proteins; to antibodies raised against such proteins; and to compounds that inhibit the activity of such proteins. The present invention also includes methods to obtain and use such proteins, nucleic acid molecules, antibodies, and inhibitory compounds. The present invention also includes therapeutic compositions comprising such inhibitory compounds, particularly those that specifically inhibit flea peritrophin activity, as well as the use of such therapeutic compositions to treat animals.

CROSS REFERENCE TO RELATED APPLICATIONS

This application is a Continuation-in-Part of U.S. patent application Ser. No. 09/543,668, filed Apr. 7, 2000, which claims priority to U.S. Provisional Patent Application Serial No. 60/128,704, filed Apr. 9, 1999, each entitled “NOVEL FLEA HEAD, NERVE CORD, HINDGUT AND MALPIGHIAN TUBULE NUCLEIC ACID MOLECULES, PROTEINS AND USES THEREOF”.

FIELD OF THE INVENTION

The present invention relates to flea peritrophin nucleic acid molecules, proteins encoded by such nucleic acid molecules, antibodies raised against such proteins, and inhibitors of such proteins. The present invention also includes methods to obtain such proteins, nucleic acid molecules, antibodies, and inhibitory compounds. The present invention also includes therapeutic compositions comprising such inhibitors, as well as uses thereof.

BACKGROUND OF THE INVENTION

Flea infestation of animals is a health and economic concern for pet owners. In particular, the bites of fleas are a problem for animals maintained as pets because the infestation becomes a source of annoyance not only for the pet but also for the pet owner who may find his or her home generally contaminated with insects. Fleas also directly cause a variety of diseases, including allergy, and also carry a variety of infectious agents including, but not limited to, endoparasites (e.g., nematodes, cestodes, trematodes and protozoa), bacteria and viruses. As such, fleas are a problem not only when they are on an animal but also when they are in the general environment of the animal.

The medical importance of flea infestation has prompted the development of reagents capable of controlling flea infestation. Commonly encountered methods to control flea infestation are generally focused on use of insecticides, which are often unsuccessful for one or more of the following reasons: (1) failure of owner compliance (frequent administration is required); (2) behavioral or physiological intolerance of the pet to the pesticide product or means of administration; and (3) the emergence of flea populations resistant to the prescribed dose of pesticide.

Peritrophins, including flea PL1, PL2, PL3, PL4 and PL5 proteins of the present invention, are a family of putative chitin-binding proteins that comprise a structural component of the peritrophic matrix, an acellular membrane composed of proteins and sugars, most commonly chitin which forms a barrier between the contents of an ingested meal and the gut epithelia. Peritrophin-like proteins have also been shown to be present in the trachea of Drosophila embryos, indicating that such proteins may have additional roles outside the midgut. The function of the peritrophin-like proteins in adult fleas is not clear, since adult fleas do not produce a peritrophic matrix in the gut. Peritrophins have been investigated as targets for immunological control of hematophagous insects including the sheep blowfly, Lucilia cuprina. It has been shown in this insect that ingestion of antibodies against peritrophins inhibits the growth of larvae and can result in increased larval mortality. It has also been shown that the ingestion of antibodies against peritrophins reduces the permeability of the peritrophic matrix in L. cuprina larvae. This in turn may inhibit the movement of digested food across the peritrophic matrix to the gut epithelium, resulting in starvation. As such, a flea peritrophin of the present invention represents a novel target for anti-flea vaccines and chemotherapeutic drugs.

Therefore, isolation and sequencing of flea peritrophin genes may be critical for use in identifying specific agents for treating animals for flea infestation.

SUMMARY OF THE INVENTION

The present invention provides flea peritrophin nucleic acid molecules, proteins encoded by such nucleic acid molecules; antibodies raised against such proteins (i.e., anti-flea peritrophin antibodies); mimetopes of such proteins or antibodies; compositions comprising such nucleic acid molecules, proteins, antibodies, and mimetopes; and compounds that inhibit flea peritrophin activity (i.e. inhibitory compounds or inhibitors).

The present invention also includes methods to obtain such proteins, mimetopes, nucleic acid molecules, antibodies and inhibitory compounds. The present invention also includes the use of proteins and antibodies to identify such inhibitory compounds as well as assay kits to identify such inhibitory compounds. Also included in the present invention are therapeutic compositions comprising proteins, mimetopes, nucleic acid molecules, antibodies and inhibitory compounds of the present invention including therapeutic compounds derived from a protein of the present invention that inhibit the activity of flea peritrophin proteins; also included are uses of such therapeutic compounds.

One embodiment of the present invention is an isolated flea peritrophin nucleic acid molecule that hybridizes with a nucleic acid sequence having SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:44, SEQ ID NO:45, SEQ ID NO:46, SEQ ID NO:47, and/or SEQ ID NO:49, under conditions that allow less than or equal to 30% base pair mismatch. Another embodiment of the present invention is an isolated flea peritrophin nucleic acid molecule having a nucleic acid sequence that is at least 70% identical to SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:44, SEQ ID NO:45, SEQ ID NO:46, SEQ ID NO:47, and/or SEQ ID NO:49 and fragments of any of such nucleic acid sequences of at least 35 nucleotides in length.

The present invention also relates to recombinant molecules, recombinant viruses and recombinant cells that include a nucleic acid molecule of the present invention. Also included are methods to produce such nucleic acid molecules, recombinant molecules, recombinant viruses and recombinant cells.

Another embodiment of the present invention includes an isolated flea peritrophin protein that is at least 70% identical to an amino acid sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:12, SEQ ID NO:17, SEQ ID NO:26, SEQ ID NO:31, SEQ ID NO:40, SEQ ID NO:43, and/or SEQ ID NO:48 and fragments thereof having at least 10 amino acid residues, wherein such fragments can elicit an immune response against respective flea peritrophin proteins or selectively binds to an antibody that binds any of such amino acid sequences.

Another embodiment of the present invention includes an isolated flea peritrophin protein encoded by a nucleic acid molecule at least 35 nucleotides in length that hybridizes with a nucleic acid sequence having SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:24, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:32, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:46, and/or SEQ ID NO:49, under conditions that allow less than or equal to 30% base pair mismatch.

Another embodiment of the present invention includes a composition comprising an excipient and a compound selected from the group consisting of nucleic acid molecules, proteins, and antibodies of the present invention and a method to treat an animal for flea infestation comprising administering such a composition to such an animal.

Another embodiment of the present invention includes a method to detect an inhibitor of flea peritrophin activity, said method comprising (a) contacting an isolated flea peritrophin protein of the present invention, with a putative inhibitory compound under conditions in which, in the absence of said compound, said protein has flea peritrophin protein activity, and (b) determining if said putative inhibitory compound inhibits flea peritrophin protein activity.

DETAILED DESCRIPTION OF THE INVENTION

The present invention provides for flea peritrophin nucleic acid molecules, proteins encoded by such nucleic acid molecules, antibodies raised against such proteins, and inhibitors of such proteins. As used herein, flea peritrophin nucleic acid molecules and proteins encoded by such nucleic acid molecules are also referred to as peritrophin nucleic acid molecules and proteins, or PL nucleic acid molecules and PL proteins, respectively. Flea peritrophin nucleic acid molecules and proteins of the present invention can be isolated from a flea or prepared recombinantly or synthetically. Flea peritrophin nucleic acid molecules of the present invention can be RNA or DNA, or modified forms thereof, and can be double-stranded or single-stranded; examples of nucleic acid molecules include, but are not limited to, complementary DNA (cDNA) molecules, genomic DNA molecules, synthetic DNA molecules, DNA molecules which are specific tags for messenger RNA, and corresponding mRNA molecules. As such, a flea nucleic acid molecule of the present invention is not intended refer to an entire chromosome within which such a nucleic acid molecule is contained, however, a flea peritrophin cDNA of the present invention may include all regions such as regulatory regions that control production of flea peritrophin proteins encoded by such a cDNA (such as, but not limited to, transcription, translation or post-translation control regions) as well as the coding region itself, and any introns or non-translated coding regions. As used herein, the phrase “flea peritrophin protein” refers to a protein encoded by a flea peritrophin nucleic acid molecule.

Peritrophins, including flea PL1, PL2, PL3, PL4 and PL5 proteins of the present invention, are a family of putative chitin-binding proteins that comprise a structural component of the peritrophic matrix, an acellular membrane composed of proteins and sugars, most commonly chitin which forms a barrier between the contents of an ingested meal and the gut epithelia. Flea peritrophin proteins of the present invention are characterized as containing a 6 cysteine motif, including a highly conserved motif at cysteine 2 through cysteine 3 of “CNNYYNC”, where “N” represents any amino acid residue and “Y” represents an aromatic amino acid residue.

Flea peritrophin nucleic acid molecules of known length isolated from a flea, such as Ctenocephalides felis are denoted “nCfPL1_(#)”, for example nCfPL1₁₀₉₆, wherein “#” refers to the number of nucleotides in that molecule, and flea peritrophin proteins of known length are denoted “PCfPL1_(#)” (for example PCfPL1₂₇₂) wherein “#” refers to the number of amino acid residues in that molecule.

The present invention also provides for flea peritrophin DNA molecules that are specific tags for messenger RNA molecules. Such DNA molecules can correspond to an entire or partial sequence of a messenger RNA, and therefore, a DNA molecule corresponding to such a messenger RNA molecule (i.e. a cDNA molecule), can encode a full-length or partial-length protein. A nucleic acid molecule encoding a partial-length protein can be used directly as a probe or indirectly to generate primers to identify and/or isolate a cDNA nucleic acid molecule encoding a corresponding, or structurally related, full-length protein. Such a partial cDNA nucleic acid molecule can also be used in a similar manner to identify a genomic nucleic acid molecule, such as a nucleic acid molecule that contains the complete gene including regulatory regions, exons and introns. Methods for using partial flea peritrophin cDNA molecules and sequences to isolate full-length and corresponding cDNA molecules are described in the examples herein below.

The proteins and nucleic acid molecules of the present invention can be obtained from their natural source, or can be produced using, for example, recombinant nucleic acid technology or chemical synthesis. Also included in the present invention is the use of these proteins and nucleic acid molecules as well as antibodies and inhibitory compounds thereto as therapeutic compositions to protect animals from flea infestation as well as in other applications, such as those disclosed below.

One embodiment of the present invention is an isolated protein that includes a flea peritrophin protein. It is to be noted that the term “a” or “an” entity refers to one or more of that entity; for example, a protein, a nucleic acid molecule, an antibody and a therapeutic composition refers to “one or more” or “at least one” protein, nucleic acid molecule, antibody and therapeutic composition respectively. As such, the terms “a” (or “an”), “one or more” and “at least one” can be used interchangeably herein. It is also to be noted that the terms “comprising”, “including”, and “having” can be used interchangeably. According to the present invention, an isolated, or biologically pure, protein, is a protein that has been removed from its natural milieu. As such, “isolated” and “biologically pure” do not necessarily reflect the extent to which the protein has been purified. An isolated protein of the present invention can be obtained from its natural source, can be produced using recombinant DNA technology, or can be produced by chemical synthesis.

As used herein, isolated flea peritrophin proteins of the present invention can be full-length proteins or any homologue of such proteins. An isolated protein of the present invention, including a homologue, can be identified in a straight-forward manner by the protein's ability to elicit an immune response against a flea peritrophin protein or by the protein's ability to exhibit flea peritrophin activity. Examples of flea peritrophin homologue proteins include flea peritrophin proteins in which amino acids have been deleted (e.g., a truncated version of the protein, such as a peptide), inserted, inverted, substituted and/or derivatized (e.g., by glycosylation, phosphorylation, acetylation, myristoylation, prenylation, palmitoylation, amidation and/or addition of glycerophosphatidyl inositol) such that the homologue includes at least one epitope capable of eliciting an immune response against a flea peritrophin protein, and/or of binding to an antibody directed against a flea peritrophin protein. That is, when the homologue is administered to an animal as an immunogen, using techniques known to those skilled in the art, the animal will produce an immune response against at least one epitope of a natural flea peritrophin protein. The ability of a protein to effect an immune response can be measured using techniques known to those skilled in the art. As used herein, the term “epitope” refers to the smallest portion of a protein or other antigen capable of selectively binding to the antigen binding site of an antibody or a T cell receptor. It is well accepted by those skilled in the art that the minimal size of a protein epitope is about four to six amino acids. As is appreciated by those skilled in the art, an epitope can include amino acids that naturally are contiguous to each other as well as amino acids that, due to the tertiary structure of the natural protein, are in sufficiently close proximity to form an epitope. According to the present invention, an epitope includes a portion of a protein comprising at least 4 amino acids, at least 5 amino acids, at least 6 amino acids, at least 10 amino acids, at least 15 amino acids, at least 20 amino acids, at least 25 amino acids, at least 30 amino acids, at least 35 amino acids, at least 40 amino acids or at least 50 amino acids in length.

Flea peritrophin homologue proteins can be the result of natural allelic variation or natural mutation. Flea peritrophin protein homologues of the present invention can also be produced using techniques known in the art including, but not limited to, direct modifications to the protein or modifications to the gene encoding the protein using, for example, classic or recombinant DNA techniques to effect random or targeted mutagenesis.

Flea peritrophin proteins of the present invention are encoded by flea peritrophin nucleic acid molecules. As used herein, flea peritrophin nucleic acid molecules include nucleic acid sequences related to natural flea peritrophin genes, and, preferably, to C. felis flea peritrophin genes. As used herein, flea peritrophin genes include all regions such as regulatory regions that control production of flea peritrophin proteins encoded by such genes (such as, but not limited to, transcription, translation or post-translation control regions) as well as the coding region itself, and any introns or non-translated coding regions. As used herein, a nucleic acid molecule that “includes” or “comprises” a sequence may include that sequence in one contiguous array, or may include the sequence as fragmented exons such as is often found for a flea gene. As used herein, the term “coding region” refers to a continuous linear array of nucleotides that translates into a protein. A full-length coding region is that coding region that is translated into a full-length, i.e., a complete protein as would be initially translated in its natural millieu, prior to any post-translational modifications.

One embodiment of the present invention is a C. felis flea peritrophin gene that includes the nucleic acid sequence SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:44, SEQ ID NO:45, SEQ ID NO:46, SEQ ID NO:47, and/or SEQ ID NO:49. These nucleic acid sequences are further described herein. For example, nucleic acid sequence SEQ ID NO:1 represents the deduced sequence of the coding strand of a C. felis cDNA denoted herein as C. felis peritrophin nucleic acid molecule nCfPL1₁₀₉₆, the production of which is disclosed in the Examples. Nucleic acid molecule SEQ ID NO:1 comprises an apparently full-length coding region. The complement of SEQ ID NO:1 (represented herein by SEQ ID NO:3) refers to the nucleic acid sequence of the strand fully complementary to the strand having SEQ ID NO:1, which can easily be determined by those skilled in the art. Likewise, a nucleic acid sequence complement of any nucleic acid sequence of the present invention refers to the nucleic acid sequence of the nucleic acid strand that is fully complementary to (i.e., can form a complete double helix with) the strand for which the sequence is cited. It should be noted that since nucleic acid sequencing technology is not entirely error-free, SEQ ID NO:1 (as well as other nucleic acid and protein sequences presented herein) represents an apparent nucleic acid sequence of the nucleic acid molecule encoding a flea peritrophin protein of the present invention.

Translation of SEQ ID NO:1, the coding strand of nCfPL1₁₀₉₆, as well as translation of SEQ ID NO:4, the coding strand of nCfPL1₈₁₆, which represents the coding region of nCfPL1₁₀₉₆, yields a protein of 272 amino acids, denoted herein as PCfPL1₂₇₂, the amino acid sequence of which is presented in SEQ ID NO:2, assuming (a) an initiation codon extending from nucleotide 6 to 8 of SEQ ID NO:1, or from nucleotide 1 to nucleotide 3 of SEQ ID NO:4, respectively; and (b) a stop codon extending from nucleotide 822 to 824 of SEQ ID NO:1.

Translation of SEQ ID NO:11, the coding strand of nCfPL2₁₄₆₅, as well as translation of SEQ ID NO:14, the coding strand of nCfPL2₁₃₅₉, which represents the coding region of nCfPL2₁₄₆₅, yields a protein of 453 amino acids, denoted herein as PCfPL2₄₅₃, the amino acid sequence of which is presented in SEQ ID NO:12, assuming (a) an initiation codon extending from nucleotide 3 to5 of SEQ ID NO:11, or from nucleotide 1 to nucleotide 3 of SEQ ID NO:14, respectively; and (b) a stop codon extending from nucleotide 1362 to 1364 of SEQ ID NO:11.

Translation of SEQ ID NO:16, the coding strand of nCfPL3₃₈₇, as well as translation of SEQ ID NO:19, the coding strand of nCfPL3₂₄₃, which represents the coding region of nCfPL3₃₈₇, yields a protein of 81 amino acids, denoted herein as PCfPL3₈₁, the amino acid sequence of which is presented in SEQ ID NO:17, assuming (a) an initiation codon extending from nucleotide 20-22 of SEQ ID NO:16, or from nucleotide 1 to nucleotide 3 of SEQ ID NO:19, respectively; and (b) a stop codon extending from nucleotide 263 to 265 of SEQ ID NO:16.

Translation of SEQ ID NO:25, the coding strand of nCfPL4₁₀₄₈, as well as translation of SEQ ID NO:28, the coding strand of nCfPL4₈₅₅, which represents the coding region of nCfPL4₁₀₄₈, yields a protein of 285 amino acids, denoted herein as PCfP4₂₈₅, the amino acid sequence of which is presented in SEQ ID NO:26, assuming (a) an initiation codon extending from nucleotide 19-21 of SEQ ID NO:25, or from nucleotide 1 to nucleotide 3 of SEQ ID NO:28, respectively; and (b) a stop codon extending from nucleotide 874 to 876 of SEQ ID NO:25.

Translation of SEQ ID NO:42, the coding strand of nCfPL5₁₈₃₂, as well as translation of SEQ ID NO:45, the coding strand of nCfPL5₁₁₉₁, which represents the coding region of nCfPL5₁₈₃₂, yields a protein of 397 amino acids, denoted herein as PCfPL5₃₉₇, the amino acid sequence of which is presented in SEQ ID NO:43, assuming (a) an initiation codon extending from nucleotide 146-148 of SEQ ID NO:42, or from nucleotide 1 to nucleotide 3 of SEQ ID NO:45, respectively; and (b) a stop codon extending from nucleotide 1337 to 1339 of SEQ ID NO:42.

In one embodiment, a gene or other nucleic acid molecule of the present invention can be an allelic variant that includes a similar but not identical sequence to SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:44, SEQ ID NO:45, SEQ ID NO:46, SEQ ID NO:47, and/or SEQ ID NO:49. For example, an allelic variant of a C. felis peritrophin gene including SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:44, SEQ ID NO:45, SEQ ID NO:46, SEQ ID NO:47, and/or SEQ ID NO:49 is a gene that occurs at essentially the same locus (or loci) in the genome as the gene including SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ iD NO:27, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:44, SEQ ID NO:45, SEQ ID NO:46, SEQ ID NO:47, and/or SEQ ID NO:49, but which, due to natural variations caused by, for example, mutation or recombination, has a similar but not identical sequence. Because natural selection typically selects against alterations that affect function, allelic variants (i.e. alleles corresponding to, or of, cited nucleic acid sequences) usually encode proteins having similar activity to that of the protein encoded by the gene to which they are being compared. Allelic variants of genes or nucleic acid molecules can also comprise alterations in the 5′ or 3′ untranslated regions of the gene (e.g., in regulatory control regions), or can involve alternative splicing of a nascent transcript, thereby bringing alternative exons into juxtaposition. Allelic variants are well known to those skilled in the art and would be expected to occur naturally within a given flea species, since the genome is diploid, and sexual reproduction will result in the reassortment of alleles.

In one embodiment of the present invention, isolated flea peritrophin proteins are encoded by nucleic acid molecules that hybridize under stringent hybridization conditions to genes or other nucleic acid molecules encoding flea peritrophin proteins, respectively. The minimal size of flea peritrophin proteins of the present invention is a size sufficient to be encoded by a nucleic acid molecule capable of forming a stable hybrid (i.e., hybridizing under stringent hybridization conditions) with the complementary sequence of a nucleic acid molecule encoding the corresponding natural protein. The size of a nucleic acid molecule encoding such a protein is dependent on the nucleic acid composition and the percent homology between the flea peritrophin nucleic acid molecule and the complementary nucleic acid sequence. It can easily be understood that the extent of homology required to form a stable hybrid under stringent conditions can vary depending on whether the homologous sequences are interspersed throughout a given nucleic acid molecule or are clustered (i.e., localized) in distinct regions on a given nucleic acid molecule.

The minimal size of a nucleic acid molecule capable of forming a stable hybrid with a gene encoding a flea peritrophin protein is at least about 12 to about 15 nucleotides in length if the nucleic acid molecule is GC-rich and at least about 15 to about 17 bases in length if it is AT-rich. The minimal size of a nucleic acid molecule used to encode a flea peritrophin protein homologue of the present invention is from about 12 to about 18 nucleotides in length. Thus, the minimal size of flea peritrophin protein homologues of the present invention is from about 4 to about 6 amino acids in length. There is no limit, other than a practical limit, on the maximal size of a nucleic acid molecule encoding a flea peritrophin protein of the present invention because a nucleic acid molecule of the present invention can include a portion of a gene or cDNA or RNA, an entire gene or cDNA or RNA, or multiple genes or cDNA or RNA. The preferred size of a protein encoded by a nucleic acid molecule of the present invention depends on whether a full-length, fusion, multivalent, or functional portion of such a protein is desired.

Stringent hybridization conditions are determined based on defined physical properties of the flea peritrophin nucleic acid molecule to which the nucleic acid molecule is being hybridized, and can be defined mathematically. Stringent hybridization conditions are those experimental parameters that allow an individual skilled in the art to identify significant similarities between heterologous nucleic acid molecules. These conditions are well known to those skilled in the art. See, for example, Sambrook, et al., 1989, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Labs Press, and Meinkoth, et al., 1984, Anal. Biochem. 138, 267-284, each of which is incorporated by reference herein in its entirety. As explained in detail in the cited references, the determination of hybridization conditions involves the manipulation of a set of variables including the ionic strength (M, in moles/liter), the hybridization temperature (° C.), the concentration of nucleic acid helix destabilizing agents (such as formamide), the average length of the shortest hybrid duplex (n), and the percent G+C composition of the fragment to which an unknown nucleic acid molecule is being hybridized. For nucleic acid molecules of at least about 150 nucleotides, these variables are inserted into a standard mathematical formula to calculate the melting temperature, or T_(m), of a given nucleic acid molecule. As defined in the formula below, T_(m) is the temperature at which two complementary nucleic acid molecule strands will disassociate, assuming 100% complementarity between the two strands:

T _(m)=81.5° C.+16.6logM+0.41(%G+C)−500/n−0.61(%formamide).

For nucleic acid molecules smaller than about 50 nucleotides, hybrid stability is defined by the dissociation temperature (T_(d)), which is defined as the temperature at which 50% of the duplexes dissociate. For these smaller molecules, the stability at a standard ionic strength is defined by the following equation:

T _(d)=4(G+C)+2(A+T).

A temperature of 5° C. below T_(d) is used to detect hybridization between perfectly matched molecules.

Also well known to those skilled in the art is how base pair mismatch, i.e. differences between two nucleic acid molecules being compared, including non-complementarity of bases at a given location, and gaps due to insertion or deletion of one or more bases at a given location on either of the nucleic acid molecules being compared, will affect T_(m) or T_(d) for nucleic acid molecules of different sizes. For example, T_(m) decreases about 1° C. for each 1% of mismatched base pairs for hybrids greater than about 150 bp, and T_(d) decreases about 5° C. for each mismatched base pair for hybrids below about 50 bp. Conditions for hybrids between about 50 and about 150 base pairs can be determined empirically and without undue experimentation using standard laboratory procedures well known to those skilled in the art. These simple procedures allow one skilled in the art to set the hybridization conditions (by altering, for example, the salt concentration, the helix destabilizing compound concentration or the temperature) so that only nucleic acid hybrids with greater than a specified % base pair mismatch will hybridize. Because one skilled in the art can easily determine whether a given nucleic acid molecule to be tested is less than or greater than about 50 nucleotides, and can therefore choose the appropriate formula for determining hybridization conditions, he or she can determine whether the nucleic acid molecule will hybridize with a given gene under conditions designed to allow a desired amount of base pair mismatch.

Hybridization reactions are often carried out by attaching the nucleic acid molecule to be hybridized to a solid support such as a membrane, and then hybridizing with a labeled nucleic acid molecule, typically referred to as a probe, suspended in a hybridization solution. Examples of common hybridization reaction techniques include, but are not limited to, the well-known Southern and northern blotting procedures. Typically, the actual hybridization reaction is done under non-stringent conditions, i.e., at a lower temperature and/or a higher salt concentration, and then high stringency is achieved by washing the membrane in a solution with a higher temperature and/or lower salt concentration in order to achieve the desired stringency.

For example, if the skilled artisan wished to identify a nucleic acid molecule that hybridizes under conditions that would allow less than or equal to 30% pair mismatch with a flea peritrophin nucleic acid molecule of about 150 bp in length or greater, the following conditions could preferably be used. The average G+C content of flea DNA is about 37%, as calculated from known flea nucleic acid sequences. The unknown nucleic acid molecules would be attached to a support membrane, and the 150 bp probe would be labeled, e.g. with a radioactive tag. The hybridization reaction could be carried out in a solution comprising 2×SSC in the absence of nucleic acid helix destabilizing compounds, at a temperature of about 37° C. (low stringency conditions). Solutions of differing concentrations of SSC can be made by one of skill in the art by diluting a stock solution of 20×SSC (175.3 gram NaCl and about 88.2 gram sodium citrate in 1 liter of water, pH 7) to obtain the desired concentration of SSC. The skilled artisan would calculate the washing conditions required to allow up to 30% base pair mismatch. For example, in a wash solution comprising 1×SSC in the absence of nucleic acid helix destabilizing compounds, the T_(m) of perfect hybrids would be about 77° C.:

81.5° C.+16.6log (0.15M)+(0.41×73)−(500/150)−(0.61×0)=77.5° C.

Thus, to achieve hybridization with nucleic acid molecules having about 30% base pair mismatch, hybridization washes would be carried out at a temperature of less than or equal to 47.5° C. It is thus within the skill of one in the art to calculate additional hybridization temperatures based on the desired percentage base pair mismatch, formulae and G/C content disclosed herein. For example, it is appreciated by one skilled in the art that as the nucleic acid molecule to be tested for hybridization against nucleic acid molecules of the present invention having sequences specified herein becomes longer than 150 nucleotides, the T_(m) for a hybridization reaction allowing up to 30% base pair mismatch will not vary significantly from 47.5° C.

Furthermore, it is known in the art that there are commercially available computer programs for determining the degree of similarity between two nucleic acid or protein sequences. These computer programs include various known methods to determine the percentage identity and the number and length of gaps between hybrid nucleic acid molecules or proteins. Preferred methods to determine the percent identity among amino acid sequences and also among nucleic acid sequences include analysis using one or more of the commercially available computer programs designed to compare and analyze nucleic acid or amino acid sequences. These computer programs include, but are not limited to, the SeqLab® Wisconsin Package™ Version 10.0-UNIX sequence analysis software, available from Genetics Computer Group, Madison, Wis. (hereinafter “SeqLab”); and DNAsis® sequence analysis software, version 2.0, available from Hitachi Software, San Bruno, Calif. (hereinafter “DNAsis”). Such software programs represent a collection of algorithms paired with a graphical user interface for using the algorithms. The DNAsis and SeqLab software, for example, employ a particular algorithm, the Needleman-Wunsch algorithm to perform pair-wise comparisons between two sequences to yield a percentage identity score, see Needleman, S. B. and Wunch, C. D., 1970, J. Mol. Biol., 48, 443, which is incorporated herein by reference in its entirety. Such algorithms, including the Needleman-Wunsch algorithm, are commonly used by those skilled in the nucleic acid and amino acid sequencing art to compare sequences. A preferred method to determine percent identity among amino acid sequences and also among nucleic acid sequences includes using the Needleman-Wunsch algorithm, available in the SeqLab software, using the Pairwise Comparison/Gap function with the nwsgapdna.cmp scoring matrix, the gap creation penalty and the gap extension penalties set at default values, and the gap shift limits set at maximum (hereinafter referred to as “SeqLab default parameters”). An additional preferred method to determine percent identity among amino acid sequences and also among nucleic acid sequences includes using the Higgins-Sharp algorithm, available in the DNAsis software, with the gap penalty set at 5, the number of top diagonals set at 5, the fixed gap penalty set at 10, the k-tuple set at 2, the window size set at 5, and the floating gap penalty set at 10. A particularly preferred method to determine percent identity among amino acid sequences and also among nucleic acid sequences includes using the Needleman-Wunsch algorithm available in the SeqLab software, using the SeqLab default parameters.

One embodiment of the present invention includes a flea peritrophin protein. A preferred flea peritrophin protein includes a protein encoded by a nucleic acid molecule that hybridizes under conditions that preferably allow less than or equal to 30% base pair mismatch, preferably under conditions that allow less than or equal to 20% base pair mismatch, preferably under conditions that allow less than or equal to 10% base pair mismatch, preferably under conditions that allow less than or equal to 8% base pair mismatch, preferably under conditions that allow less than or equal to 5% base pair mismatch or preferably under conditions that allow less than or equal to 2% base pair mismatch with a nucleic acid molecule selected from the group consisting of SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:24, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:32, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:46, and/or SEQ ID NO:49.

Another embodiment of the present invention includes a flea peritrophin protein encoded by a nucleic acid molecule that hybridizes under conditions comprising, (a) hybridizing in a solution comprising 1×SSC in the absence of nucleic acid helix destabilizing compounds, at a temperature of 37° C. and (b) washing in a solution comprising 1×SSC in the absence of nucleic acid helix destabilizing compounds, at a temperature of 47° C., to an isolated nucleic acid molecule selected from the group consisting of SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:24, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:32, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:46, and/or SEQ ID NO:49.

Another preferred flea peritrophin protein of the present invention includes a protein that is encoded by a nucleic acid molecule that is preferably at least 70% identical, preferably at least 80% identical, preferably at least 90% identical, preferably at least 92% identical, preferably at least 95% identical or preferably at least 98% identical to a nucleic acid molecule having the nucleic acid sequence SEQ ID NO:1, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO:25, SEQ ID NO:28, SEQ ID NO:30, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:42, SEQ ID NO:45, and/or SEQ ID NO:47; also preferred are fragments (i.e. portions) of such proteins encoded by nucleic acid molecules that are at least 25 nucleotides. Percent identity as used herein is determined using the Needleman-Wunsch algorithm, available in the SeqLab software using default parameters.

Additional preferred flea peritrophin proteins of the present invention include proteins having the amino acid sequence SEQ ID NO:2, SEQ ID NO:12, SEQ ID NO:17, SEQ ID NO:26, SEQ ID NO:31, SEQ ID NO:40, SEQ ID NO:43, and/or SEQ ID NO:48, and proteins comprising homologues of a protein having the amino acid sequence SEQ ID NO:2, SEQ ID NO:12, SEQ ID NO:17, SEQ ID NO:26, SEQ ID NO:31, SEQ ID NO:40, SEQ ID NO:43, and/or SEQ ID NO:48, wherein such a homologue comprises at least one epitope that elicits an immune response against a protein having an amino acid sequence SEQ ID NO:2, SEQ ID NO:12, SEQ ID NO:17, SEQ ID NO:26, SEQ ID NO:31, SEQ ID NO:40, SEQ ID NO:43, and/or SEQ ID NO:48. Likewise, also preferred are proteins encoded by nucleic acid molecules comprising nucleic acid sequence SEQ ID NO:1, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO:25, SEQ ID NO:28, SEQ ID NO:30, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:42, SEQ ID NO:45, and/or SEQ ID NO:47, or by homologues thereof.

A preferred isolated flea peritrophin protein of the present invention is a protein encoded by at least one of the following nucleic acid molecules: nCfPL1₁₀₉₆, nCfPL1₈₁₆, nCfPL2₄₄₅, nCfPL2₁₂₇₉, nCfPL2₂₇₉, nCfPL2₁₄₆₅, nCfPL2₁₃₅₉, nCfPL3₃₈₇, nCfPL3₂₄₃, nCfPL4₉₆₀, nCfPL4₁₀₂₉, nCfPL4₁₀₄₈, nCfPL4₈₅₅, nCfPL4₈₀₂, nCfPL5₁₅₁₃, nCfPL5₁₈₃₂, nCfPL5₁₁₉₁, and/or nCfPL5₁₁₆₁, or allelic variants of any of these nucleic acid molecules. Also preferred is an isolated protein encoded by a nucleic acid molecule having nucleic acid sequence SEQ ID NO:1, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO:25, SEQ ID NO:28, SEQ ID NO:30, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:42, SEQ ID NO:45, and/or SEQ ID NO:47; or a protein encoded by an allelic variant of any of these listed nucleic acid molecules.

Preferred flea peritrophin proteins of the present invention include proteins having amino acid sequences that are at least 70%, preferably 80%, preferably 90%, preferably 92%, preferably 95%, preferably at least 98%, preferably at least 99%, or preferably 100% identical to amino acid sequence SEQ ID NO:2, SEQ ID NO:12, SEQ ID NO:17, SEQ ID NO:26, SEQ ID NO:31, SEQ ID NO:40, SEQ ID NO:43, and/or SEQ ID NO:48; and proteins encoded by allelic variants of nucleic acid molecules encoding flea peritrophin proteins having amino acid sequences SEQ ID NO:2, SEQ ID NO:12, SEQ ID NO:17, SEQ ID NO:26, SEQ ID NO:31, SEQ ID NO:40, SEQ ID NO:43, and/or SEQ ID NO:48. Also preferred are fragments thereof having at least 10 amino acid residues.

In one embodiment of the present invention, C. felis peritrophin proteins comprise amino acid sequence SEQ ID NO:2, SEQ ID NO:12, SEQ ID NO:17, SEQ ID NO:26, SEQ ID NO:31, SEQ ID NO:40, SEQ ID NO:43, and/or SEQ ID NO:48 (including, but not limited to, the proteins consisting of amino acid sequence SEQ ID NO:2, SEQ ID NO:12, SEQ ID NO:17, SEQ ID NO:26, SEQ ID NO:31, SEQ ID NO:40, SEQ ID NO:43, and/or SEQ ID NO:48, fusion proteins and multivalent proteins), and proteins encoded by allelic variants of nucleic acid molecules encoding proteins having amino acid sequence SEQ ID NO:2, SEQ ID NO:12, SEQ ID NO:17, SEQ ID NO:26, SEQ ID NO:31, SEQ ID NO:40, SEQ ID NO:43, and/or SEQ ID NO:48.

In one embodiment, a preferred flea peritrophin protein comprises an amino acid sequence of at least 6 amino acids, preferably at least 10 amino acids, preferably at least 15 amino acids, preferably at least 20 amino acids, preferably at least 25 amino acids, preferably at least 30 amino acids, preferably at least 35 amino acids, preferably at least 40 amino acids, preferably at least 50 amino acids, preferably at least 75 amino acids, preferably at least 100 amino acids, preferably at least 125 amino acids, preferably at least 150 amino acids, preferably at least 175 amino acids, preferably at least 200 amino acids, preferably at least 250 amino acids, preferably at least 300 amino acids, preferably at least 350 amino acids, preferably at least 400 amino acids, or preferably at least 450 amino acids. In another embodiment, preferred flea peritrophin proteins comprise full-length proteins, i.e., proteins encoded by full-length coding regions, or post-translationally modified proteins thereof, such as mature proteins from which initiating methionine and/or signal sequences or “pro” sequences have been removed.

Additional preferred flea peritrophin proteins of the present invention include proteins encoded by nucleic acid molecules comprising at least a portion of nCfPL1₁₀₉₆, nCfPL1₈₁₆, nCfPL2₄₄₅, nCfPL2₁₂₇₉, nCfPL2₂₇₉, nCfPL2₁₄₆₅, nCfPL2₁₃₅₉, nCfPL3₃₈₇, nCfPL3₂₄₃, nCfPL4₉₆₀, nCfPL4₁₀₂₉, nCfPL4₁₀₄₈, nCfPL4₈₅₅, nCfPL4₈₀₂, nCfPL5₁₅₁₃, nCfPL5₁₈₃₂, nCfPL5₁₁₉₁, and/or nCfPL5₁₁₆₁, as well as flea peritrophin proteins encoded by allelic variants of such nucleic acid molecules. A portion of such flea peritrophin nucleic acid molecule is preferably at least 25 nucleotides in length.

Also preferred are flea peritrophin proteins encoded by nucleic acid molecules having nucleic acid sequences comprising at least a portion of SEQ ID NO:1, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO:25, SEQ ID NO:28, SEQ ID NO:30, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:42, SEQ ID NO:45, and/or SEQ ID NO:47, as well as allelic variants of these nucleic acid molecules. A portion of such flea peritrophin nucleic acid molecule is preferably at least 25 nucleotides in length.

In another embodiment, a preferred flea peritrophin protein of the present invention is encoded by a nucleic acid molecule comprising at least 20 nucleotides, preferably at least 25 nucleotides, preferably at least 30 nucleotides, preferably at least 40 nucleotides, preferably at least 50 nucleotides, preferably at least 75 nucleotides, preferably at least 100 nucleotides, preferably at least 125 nucleotides, preferably at least 150 nucleotides, preferably at least 175 nucleotides, preferably at least 200 nucleotides, preferably at least 250 nucleotides, preferably at least 350 nucleotides, preferably at least 450 nucleotides, preferably at least 550 nucleotides, preferably at least 650 nucleotides, preferably at least 750 nucleotides, preferably at least 1000 nucleotides, preferably at least 1100 nucleotides, preferably at least 1200 nucleotides, preferably at least 1500 nucleotides, preferably at least 1750 nucleotides, or preferably at least 1850 nucleotides in length. Within this embodiment is a flea peritrophin protein encoded by at least a portion of nCfPL1₁₀₉₆, nCfPL1₈₁₆, nCfPL2₄₄₅, nCfPL2₁₂₇₉, nCfPL2₂₇₉, nCfPL2₁₄₆₅, nCfPL2₁₃₅₉, nCfPL3₃₈₇, nCfPL3₂₄₃, nCfPL4₉₆₀, nCfPL4₁₀₂₉, nCfPL4₁₀₄₈, nCfPL4₈₅₅, nCfPL4₈₀₂, nCfPL5₁₅₁₃, nCfPL5₁₈₃₂, nCfPL5₁₁₉₁, and/or nCfPL5₁₁₆₁, or by an a allelic variant of any of these nucleic acid molecules. Preferred flea peritrophin proteins of the present invention are encoded by nucleic acid molecules comprising apparently full-length flea peritrophin coding region, i.e., nucleic acid molecules encoding an apparently full-length flea peritrophin protein.

Preferred flea peritrophin proteins of the present invention can be used to develop inhibitors that, when administered to an animal in an effective manner, are capable of protecting that animal from flea infestation. In accordance with the present invention, the ability of an inhibitor of the present invention to protect an animal from flea infestation refers to the ability of that protein to, for example, treat, ameliorate and/or prevent infestation caused by fleas. In particular, the phrase “to protect an animal from flea infestation” refers to reducing the potential for flea population expansion on and around the animal (i.e., reducing the flea burden). Preferably, the flea population size is decreased, optimally to an extent that the animal is no longer bothered by fleas. A host animal, as used herein, is an animal from which fleas can feed by attaching to and feeding through the skin of the animal. Fleas, and other ectoparasites, can live on a host animal for an extended period of time or can attach temporarily to an animal in order to feed. At any given time, a certain percentage of a flea population can be on a host animal whereas the remainder can be in the environment of the animal. Such an environment can include not only adult fleas, but also flea eggs and/or flea larvae. The environment can be of any size such that fleas in the environment are able to jump onto and off of a host animal. For example, the environment of an animal can include plants, such as crops, from which fleas infest an animal. As such, it is desirable not only to reduce the flea burden on an animal per se, but also to reduce the flea burden in the environment of the animal.

Suitable fleas to target include any flea that is essentially incapable of causing disease in an animal administered an inhibitor of the present invention. As such, fleas to target include any flea that produces a protein that can be targeted by an inhibitory compound that inhibits a flea flea peritrophin protein function, thereby resulting in the decreased ability of the parasite to cause disease in an animal. Preferred fleas to target include fleas of the following genera: Ctenocephalides, Cyopsyllus, Diamanus (Oropsylla), Echidnophaga, Nosopsyllus, Pulex, Tunga, and Xenopsylla, with those of the species Ctenocephalides canis, Ctenocephalides felis, Diamanus montanus, Echidnophaga gallinacea, Nosopsyllus faciatus, Pulex irritans, Pulex simulans, Tunga penetrans and Xenopsylla cheopis being more preferred, with C. felis being even more preferred. Such fleas are also preferred for the isolation of proteins or nucleic acid molecules of the present invention.

One embodiment of a flea peritrophin protein of the present invention is a fusion protein that includes a flea peritrophin protein-containing domain attached to one or more fusion segments. Suitable fusion segments for use with the present invention include, but are not limited to, segments that can: enhance a protein's stability; act as an immunopotentiator; and/or assist in purification of a flea peritrophin protein (e.g., by affinity chromatography). A suitable fusion segment can be a domain of any size that has the desired function (e.g., imparts increased stability, imparts increased immunogenicity to a protein, and/or simplifies purification of a protein). Fusion segments can be joined to amino and/or carboxyl termini of the flea peritrophin-containing domain of the protein and can be susceptible to cleavage in order to enable straight-forward recovery of a flea peritrophin protein. Fusion proteins are preferably produced by culturing a recombinant cell transformed with a fusion nucleic acid molecule that encodes a protein including the fusion segment attached to either the carboxyl and/or amino terminal end of a flea peritrophin-containing domain. Preferred fusion segments include a metal binding domain (e.g., a poly-histidine segment); an immunoglobulin binding domain (e.g., Protein A; Protein G; T cell; B cell; Fc receptor or complement protein antibody-binding domains); a sugar binding domain (e.g., a maltose binding domain); and/or a “tag” domain (e.g., at least a portion of β-galactosidase, a strep tag peptide, a T7 tag peptide, a Flag™ peptide, or other domains that can be purified using compounds that bind to the domain, such as monoclonal antibodies). More preferred fusion segments include metal binding domains, such as a poly-histidine segment; a maltose binding domain; a strep tag peptide, such as that available from Biometra in Tampa, Fla.; and an S10 peptide.

The present invention also includes mimetopes of flea peritrophin proteins of the present invention. As used herein, a mimetope of a flea peritrophin protein of the present invention refers to any compound that is able to mimic the activity of such a flea peritrophin protein, often because the mimetope has a structure that mimics the particular flea peritrophin protein. Mimetopes can be, but are not limited to: peptides that have been modified to decrease their susceptibility to degradation such as all-D retro peptides; anti-idiotypic and/or catalytic antibodies, or fragments thereof; non-proteinaceous immunogenic portions of an isolated protein (e.g., carbohydrate structures); and synthetic or natural organic molecules, including nucleic acids. Such mimetopes can be designed using computer-generated structures of proteins of the present invention. Mimetopes can also be obtained by generating random samples of molecules, such as oligonucleotides, peptides or other organic molecules, and screening such samples by affinity chromatography techniques using the corresponding binding partner.

Another embodiment of the present invention is an isolated nucleic acid molecule comprising a flea peritrophin nucleic acid molecule, i.e. a nucleic acid molecule that can be isolated from a flea cDNA library. As used herein, flea peritrophin nucleic acid molecules has the same meaning as flea peritrophin nucleic acid molecule. The identifying characteristics of such nucleic acid molecules are heretofore described. A nucleic acid molecule of the present invention can include an isolated natural flea peritrophin gene or a homologue thereof, the latter of which is described in more detail below. A nucleic acid molecule of the present invention can include one or more regulatory regions, full-length or partial coding regions, or combinations thereof. The minimal size of a nucleic acid molecule of the present invention is a size sufficient to allow the formation of a stable hybrid (i.e., hybridization under stringent hybridization conditions) with the complementary sequence of another nucleic acid molecule. As such, the minimal size of a flea peritrophin nucleic acid molecule of the present invention is from 12 to 18 nucleotides in length.

In accordance with the present invention, an isolated nucleic acid molecule is a nucleic acid molecule that has been removed from its natural milieu (i.e., that has been subjected to human manipulation) and can include DNA, RNA, or derivatives of either DNA or RNA. As such, “isolated” does not reflect the extent to which the nucleic acid molecule has been purified. Isolated flea peritrophin nucleic acid molecules of the present invention, or homologues thereof, can be isolated from a natural source or produced using recombinant DNA technology (e.g., polymerase chain reaction (PCR) amplification or cloning) or chemical synthesis. Isolated flea peritrophin nucleic acid molecules, and homologues thereof, can include, for example, natural allelic variants and nucleic acid molecules modified by nucleotide insertions, deletions, substitutions, and/or inversions in a manner such that the modifications do not substantially interfere with the nucleic acid molecule's ability to encode a flea peritrophin protein of the present invention.

A flea peritrophin nucleic acid molecule homologue can be produced using a number of methods known to those skilled in the art, see, for example, Sambrook et al., ibid., which is incorporated by reference herein in its entirety. For example, nucleic acid molecules can be modified using a variety of techniques including, but not limited to, classic mutagenesis and recombinant DNA techniques such as site-directed mutagenesis, chemical treatment, restriction enzyme cleavage, ligation of nucleic acid fragments, PCR amplification, synthesis of oligonucleotide mixtures and ligation of mixture groups to “build” a mixture of nucleic acid molecules, and combinations thereof. Nucleic acid molecule homologues can be selected by hybridization with flea peritrophin nucleic acid molecules or by screening the function of a protein encoded by the nucleic acid molecule (e.g., ability to elicit an immune response against at least one epitope of a flea peritrophin protein, to selectively bind to an antibody that binds a flea peritrophin protein or to effect flea peritrophin activity).

An isolated flea peritrophin nucleic acid molecule of the present invention can include a nucleic acid sequence that encodes at least one flea peritrophin protein of the present invention respectively, examples of such proteins being disclosed herein. Although the phrase “nucleic acid molecule” primarily refers to the physical nucleic acid molecule and the phrase “nucleic acid sequence” primarily refers to the sequence of nucleotides on the nucleic acid molecule, the two phrases can be used interchangeably, especially with respect to a nucleic acid molecule, or a nucleic acid sequence, being capable of encoding a flea peritrophin protein.

A preferred nucleic acid molecule of the present invention, when administered to an animal, is capable of protecting that animal from flea infestation. As will be disclosed in more detail below, a nucleic acid molecule of the present invention can be, or encode, an antisense RNA, a molecule capable of triple helix formation, a ribozyme, or other nucleic acid-based drug compound. In additional embodiments, a nucleic acid molecule of the present invention can encode a protective protein (e.g., a flea peritrophin protein of the present invention), the nucleic acid molecule being delivered to the animal, for example, by direct injection (i.e, as a genetic vaccine) or in a vehicle such as a recombinant virus vaccine or a recombinant cell vaccine.

In one embodiment of the present invention, a preferred flea peritrophin nucleic acid molecule includes an isolated nucleic acid molecule that hybridizes under conditions that preferably allow less than or equal to 30% base pair mismatch, preferably under conditions that allow less than or equal to 20% base pair mismatch, preferably under conditions that allow less than or equal to 10% base pair mismatch preferably under conditions that allow less than or equal to 5% base pair mismatch or preferably under conditions that allow less than or equal to 2% base pair mismatch with a nucleic acid molecule selected from the group consisting of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ IDNO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:44, SEQ ID NO:45, SEQ ID NO:46, SEQ ID NO:47, and/or SEQ ID NO:49.

Another embodiment of the present invention includes a flea peritrophin nucleic acid molecule, wherein said nucleic acid molecule hybridizes under conditions comprising, (a) hybridizing in solution comprising 1×SSC in the absence of nucleic acid helix destabilizing compounds, at a temperature of 37° C. and (b) washing in a solution comprising 1×SSC in the absence of nucleic acid helix destabilizing compounds, at a temperature of 47° C., to an isolated nucleic acid molecule selected from the group consisting of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:44, SEQ ID NO:45, SEQ ID NO:46, SEQ ID NO:47, and/or SEQ ID NO:49. Additional preferred nucleic acid molecules of the present invention include oligonucleotides of an isolated nucleic acid molecule, wherein said nucleic acid molecule hybridizes under conditions comprising, (a) hybridizing in solution comprising 1×SSC in the absence of nucleic acid helix destabilizing compounds, at a temperature of 37° C. and (b) washing in a solution comprising 1×SSC in the absence of nucleic acid helix destabilizing compounds, at a temperature of 47° C., to an isolated nucleic acid molecule selected from the group consisting of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:44, SEQ ID NO:45, SEQ ID NO:46, SEQ ID NO:47, and/or SEQ ID NO:49, wherein said oligonucleotide comprises at least 25 nucleotides.

Additional preferred flea peritrophin nucleic acid molecules of the present invention include nucleic acid molecules comprising a nucleic acid sequence that is preferably at least 70%, preferably at least 80%, preferably at least 90%, preferably at least 92%, preferably at least 95%, or preferably at least 98% identical to a nucleic acid sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO: 10, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:44, SEQ ID NO:45, SEQ ID NO:46, SEQ ID NO:47, and/or SEQ ID NO:49. Also preferred are oligonucleotides of any of such nucleic acid molecules. Percent identity as used herein is determined using the Needleman-Wunsch algorithm, available in the SeqLab software using default parameters.

One embodiment of the present invention is a nucleic acid molecule comprising all or part of nucleic acid molecules nCfPL1₁₀₉₆, nCfPL1₈₁₆, nCfPL2₄₄₅, nCfPL2₁₂₇₉, nCfPL2₂₇₉, nCfPL2₁₄₆₅, nCfPL2₁₃₅₉, nCfPL3₃₈₇, nCfPL3₂₄₃, nCfPL4₉₆₀, nCfPL4₁₀₂₉, nCfPL4₁₀₄₈, nCfPL4₈₅₅, nCfPL4₈₀₂, nCfPL5₁₅₁₃, nCfPL5₁₈₃₂, nCfPL5₁₁₉₁, and/or nCfPL5₁₁₆₁, or allelic variants of these nucleic acid molecules. Another preferred nucleic acid molecule of the present invention includes at least a portion of nucleic acid sequence SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:44, SEQ ID NO:45, SEQ ID NO:46, SEQ ID NO:47, and/or SEQ ID NO:49, as well as allelic variants of nucleic acid molecules having these nucleic acid sequences and homologues of nucleic acid molecules having these nucleic acid sequences; preferably such a homologue encodes or is complementary to a nucleic acid molecule that encodes at least one epitope that elicits an immune response against a protein having an amino acid sequence SEQ ID NO:2, SEQ ID NO:12, SEQ ID NO:17, SEQ ID NO:26, SEQ ID NO:31, SEQ ID NO:40, SEQ ID NO:43, and/or SEQ ID NO:48. Such nucleic acid molecules can include nucleotides in addition to those included in the SEQ ID NOs, such as, but not limited to, a full-length gene, a full-length coding region, a nucleic acid molecule encoding a fusion protein, or a nucleic acid molecule encoding a multivalent protective compound.

In one embodiment, a flea peritrophin nucleic acid molecule of the present invention encodes a protein having an amino acid sequence that is at least 70%, preferably at least 80%, preferably at least 90%, preferably at least 95%, preferably at least 98%, preferably at least 99%, or preferably at least 100% identical to SEQ ID NO:2, SEQ ID NO:12, SEQ ID NO:17, SEQ ID NO:26, SEQ ID NO:31, SEQ ID NO:40, SEQ ID NO:43, and/or SEQ ID NO:48. The present invention also includes a flea peritrophin nucleic acid molecule encoding a protein having at least a portion of SEQ ID NO:2, SEQ ID NO:12, SEQ ID NO:17, SEQ ID NO:26, SEQ ID NO:31, SEQ ID NO:40, SEQ ID NO:43, and/or SEQ ID NO:48, as well as allelic variants of a nucleic acid molecule encoding a protein having these sequences, including nucleic acid molecules that have been modified to accommodate codon usage properties of the cells in which such nucleic acid molecules are to be expressed.

In another embodiment, a preferred flea peritrophin nucleic acid molecule of the present invention comprises a nucleic acid molecule comprising at least 20 nucleotides, preferably at least 25 nucleotides, preferably at least 30 nucleotides, preferably at least 40 nucleotides, preferably at least 50 nucleotides, preferably at least 75 nucleotides, preferably at least 100 nucleotides, preferably at least 125 nucleotides, preferably at least 150 nucleotides, preferably at least 175 nucleotides, preferably at least 200 nucleotides, preferably at least 250 nucleotides, preferably at least 350 nucleotides, preferably at least 450 nucleotides, preferably at least 550 nucleotides, preferably at least 650 nucleotides, preferably at least 750 nucleotides, preferably at least 1000 nucleotides, preferably at least 1100 nucleotides, preferably at least 1200 nucleotides, preferably at least 1500 nucleotides, preferably at least 1750 nucleotides, or preferably at least 1850 nucleotides in length.

In another embodiment, a preferred flea peritrophin nucleic acid molecule encodes a protein comprising at least 6 amino acids, preferably at least 10 amino acids, preferably at least 20 amino acids, preferably at least 30 amino acids, preferably at least 40 amino acids, preferably at least 50 amino acids, preferably at least 75 amino acids, preferably at least 100 amino acids, preferably at least 200 amino acids, preferably at least 300 amino acids, preferably at least 400 amino acids, or preferably at least 450 amino acids.

In another embodiment, a preferred flea peritrophin nucleic acid molecule of the present invention comprises an apparently full-length flea peritrophin coding region, i.e., the preferred nucleic acid molecule encodes an apparently full-length flea peritrophin protein, respectively, or a post-translationally modified protein thereof. In one embodiment, a preferred flea peritrophin nucleic acid molecule of the present invention encodes a mature protein.

In another embodiment, a preferred flea peritrophin nucleic acid molecule of the present invention comprises a nucleic acid molecule comprising SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:44, SEQ ID NO:45, SEQ ID NO:46, SEQ ID NO:47, and/or SEQ ID NO:49, or a fragment thereof.

A fragment of a flea peritrophin nucleic acid molecule of the present invention preferably comprises at least 15 nucleotides, preferably at least 18 nucleotides, preferably at least 21 nucleotides, preferably at least 25 nucleotides, preferably at least 30 nucleotides, preferably at least 35 nucleotides, preferably at least 40 nucleotides, preferably at least 50 nucleotides, preferably at least 75 nucleotides, preferably at least 100 nucleotides, preferably at least 125 nucleotides, preferably at least 150 nucleotides, preferably at least 175 nucleotides, preferably at least 200 nucleotides, preferably at least 250 nucleotides, preferably at least 350 nucleotides, preferably at least 450 nucleotides, preferably at least 550 nucleotides, preferably at least 650 nucleotides, preferably at least 750 nucleotides, preferably at least 1000 nucleotides, preferably at least 1100 nucleotides, preferably at least 1200 nucleotides, preferably at least 1500 nucleotides, preferably at least 1750 nucleotides, or preferably at least 1850 nucleotides identical in sequence to a corresponding contiguous sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:44, SEQ ID NO:45, SEQ ID NO:46, SEQ ID NO:47, and/or SEQ ID NO:49.

The phrase, a nucleic acid molecule comprising at least “x” contiguous, or consecutive nucleotides identical in sequence to at least “x” contiguous, or consecutive nucleotides of a nucleic acid molecule selected from the group consisting of SEQ ID NO:“y”, refers to an “x”-nucleotide in length nucleic acid molecule that is identical in sequence to an “x”-nucleotide portion of SEQ ID NO:“y”, as well as to nucleic acid molecules that are longer in length than “x”. The additional length may be in the form of nucleotides that extend from either the 5′ or the 3′ end(s) of the contiguous identical “x”-nucleotide portion. The 5′ and/or 3′ extensions can include one or more extensions that have no identity to a molecule of the present invention, as well as extensions that show similarity or identity to cited nucleic acids sequences or portions thereof.

Knowing the nucleic acid sequences of certain flea peritrophin nucleic acid molecules of the present invention allows one skilled in the art to, for example, (a) make copies of those nucleic acid molecules, (b) obtain nucleic acid molecules including at least a portion of such nucleic acid molecules (e.g., nucleic acid molecules including full-length genes, full-length coding regions, regulatory control sequences, truncated coding regions), and (c) obtain other flea peritrophin nucleic acid molecules. Such nucleic acid molecules can be obtained in a variety of ways including screening appropriate expression libraries with antibodies of the present invention; traditional cloning techniques using oligonucleotide probes of the present invention to screen appropriate libraries; and PCR amplification of appropriate libraries or DNA using oligonucleotide primers of the present invention. Preferred libraries to screen or from which to amplify nucleic acid molecules include cDNA libraries as well as genomic DNA libraries. Similarly, preferred DNA sources to screen or from which to amplify nucleic acid molecules include cDNA and genomic DNA. Techniques to clone and amplify genes are disclosed, for example, in Sambrook et al., ibid.

The present invention also includes nucleic acid molecules that are oligonucleotides capable of hybridizing, under stringent hybridization conditions, with complementary regions of other, preferably longer, nucleic acid molecules of the present invention such as those comprising C. felis peritrophin nucleic acid molecules or other flea peritrophin nucleic acid molecules. Oligonucleotides of the present invention can be RNA, DNA, or derivatives of either. The minimum size of such oligonucleotides is the size required for formation of a stable hybrid between an oligonucleotide and a complementary sequence on a nucleic acid molecule of the present invention. A preferred oligonucleotide of the present invention has a maximum size of preferably 100 to 200 nucleotides. The present invention includes oligonucleotides that can be used as, for example, probes to identify nucleic acid molecules, primers to produce nucleic acid molecules, or therapeutic reagents to inhibit flea peritrophin protein production or activity (e.g., as antisense-, triplex formation-, ribozyme- and/or RNA drug-based reagents). The present invention also includes the use of such oligonucleotides to protect animals from disease using one or more of such technologies. Appropriate oligonucleotide-containing therapeutic compositions can be administered to an animal using techniques known to those skilled in the art.

One embodiment of the present invention includes a recombinant vector, which includes at least one isolated nucleic acid molecule of the present invention, inserted into any vector capable of delivering the nucleic acid molecule into a host cell. Such a vector contains heterologous nucleic acid sequences, that is nucleic acid sequences that are not naturally found adjacent to nucleic acid molecules of the present invention and that preferably are derived from a species other than the species from which the nucleic acid molecule(s) are derived. The vector can be either RNA or DNA, either prokaryotic or eukaryotic, and typically is a virus or a plasmid. Recombinant vectors can be used in the cloning, sequencing, and/or otherwise manipulating of flea peritrophin nucleic acid molecules of the present invention.

One type of recombinant vector, referred to herein as a recombinant molecule, comprises a nucleic acid molecule of the present invention operatively linked to an expression vector. The phrase operatively linked refers to insertion of a nucleic acid molecule into an expression vector in a manner such that the molecule is able to be expressed when transformed into a host cell. As used herein, an expression vector is a DNA or RNA vector that is capable of transforming a host cell and of effecting expression of a specified nucleic acid molecule. Preferably, the expression vector is also capable of replicating within the host cell. Expression vectors can be either prokaryotic or eukaryotic, and are typically viruses or plasmids. Expression vectors of the present invention include any vectors that function (i.e., direct gene expression) in recombinant cells of the present invention, including in bacterial, fungal, parasite, insect, other animal, and plant cells. Preferred expression vectors of the present invention can direct gene expression in bacterial, yeast, insect and mammalian cells, and more preferably in the cell types disclosed herein.

In particular, expression vectors of the present invention contain regulatory sequences such as transcription control sequences, translation control sequences, origins of replication, and other regulatory sequences that are compatible with the recombinant cell and that control the expression of nucleic acid molecules of the present invention. In particular, recombinant molecules of the present invention include transcription control sequences. Transcription control sequences are sequences that control the initiation, elongation, and termination of transcription. Particularly important transcription control sequences are those which control transcription initiation, such as promoter, enhancer, operator and repressor sequences. Suitable transcription control sequences include any transcription control sequence that can function in at least one of the recombinant cells of the present invention. A variety of such transcription control sequences are known to those skilled in the art. Preferred transcription control sequences include those that function in bacterial, yeast, or insect and mammalian cells, such as, but not limited to, tac, lac, trp, trc, oxy-pro, omp/lpp, rrnB, bacteriophage lambda (such as lambda p_(L) and lambda p_(R) and fusions that include such promoters), bacteriophage T7, T7lac, bacteriophage T3, bacteriophage SP6, bacteriophage SP01, metallothionein, alpha-mating factor, Pichia alcohol oxidase, alphavirus subgenomic promoter, antibiotic resistance gene, baculovirus, Heliothis zea insect virus, vaccinia virus, herpesvirus, raccoon poxvirus, other poxvirus, adenovirus, cytomegalovirus (such as immediate early promoter), simian virus 40, retrovirus, actin, retroviral long terminal repeat, Rous sarcoma virus, heat shock, phosphate and nitrate transcription control sequences as well as other sequences capable of controlling gene expression in prokaryotic or eukaryotic cells. Additional suitable transcription control sequences include tissue-specific promoters and enhancers as well as lymphokine-inducible promoters (e.g., promoters inducible by interferons or interleukins). Transcription control sequences of the present invention can also include naturally occurring transcription control sequences naturally associated with fleas, such as C. felis transcription control sequences.

Suitable and preferred nucleic acid molecules to include in recombinant vectors of the present invention are as disclosed herein. Preferred nucleic acid molecules to include in recombinant vectors, and particularly in recombinant molecules, include nCfPL1₁₀₉₆, nCfPL1₈₁₆, nCfPL2₄₄₅, nCfPL2₁₂₇₉, nCfPL2₂₇₉, nCfPL2₁₄₆₅, nCfPL2₁₃₅₉, nCfPL3₃₈₇, nCfPL3₂₄₃, nCfPL4₉₆₀, nCfPL4₁₀₂₉, nCfPL4₁₀₄₈, nCfPL4₈₅₅, nCfPL4₈₀₂, nCfPL5₁₅₁₃, nCfPL5₁₈₃₂, nCfPL5₁₁₉₁, and/or nCfPL5₁₁₆₁.

Recombinant molecules of the present invention may also (a) contain secretory signals (i.e., signal segment nucleic acid sequences) to enable an expressed flea peritrophin protein of the present invention to be secreted from the cell that produces the protein and/or (b) contain fusion sequences which lead to the expression of nucleic acid molecules of the present invention as fusion proteins. Examples of suitable signal segments include any signal segment capable of directing the secretion of a protein of the present invention. Preferred signal segments include, but are not limited to, tissue plasminogen activator (t-PA), interferon, interleukin, growth hormone, histocompatibility and viral envelope glycoprotein signal segments. Suitable fusion segments encoded by fusion segment nucleic acids are disclosed herein. In addition, a nucleic acid molecule of the present invention can be joined to a fusion segment that directs the encoded protein to the proteosome, such as a ubiquitin fusion segment. Eukaryotic recombinant molecules may also include intervening and/or untranslated sequences surrounding and/or within the nucleic acid sequences of nucleic acid molecules of the present invention.

Another embodiment of the present invention includes a recombinant cell comprising a host cell transformed with one or more recombinant molecules of the present invention. Transformation of a nucleic acid molecule into a cell can be accomplished by any method by which a nucleic acid molecule can be inserted into the cell. Transformation techniques include, but are not limited to, transfection, electroporation, microinjection, lipofection, adsorption, and protoplast fusion. A recombinant cell may remain unicellular or may grow into a tissue, organ or a multicellular organism. It is to be noted that a cell line refers to any recombinant cell of the present invention that is not a transgenic animal. Transformed nucleic acid molecules of the present invention can remain extrachromosomal or can integrate into one or more sites within a chromosome of the transformed (i.e., recombinant) cell in such a manner that their ability to be expressed is retained. Preferred nucleic acid molecules with which to transform a cell include flea peritrophin nucleic acid molecules disclosed herein. Preferred nucleic acid molecules with which to transform a cell include nCfPL1₁₀₉₆, nCfPL1₈₁₆, nCfPL2₄₄₅, nCfPL2₁₂₇₉, nCfPL2₂₇₉, nCfPL2₁₄₆₅, nCfPL2₁₃₅₉, nCfPL3₃₈₇, nCfPL3₂₄₃, nCfPL4₉₆₀, nCfPL4₁₀₂₉, nCfPL4₁₀₄₈, nCfPL4₈₅₅, nCfPL4₈₀₂, nCfPL5₁₅₁₃, nCfPL5₁₈₃₂, nCfPL5₁₁₉₁, and/or nCfPL5₁₁₆₁.

Suitable host cells to transform include any cell that can be transformed with a nucleic acid molecule of the present invention. Host cells can be either untransformed cells or cells that are already transformed with at least one nucleic acid molecule (e.g., nucleic acid molecules encoding one or more proteins of the present invention and/or other proteins useful in the production of multivalent vaccines). Host cells of the present invention either can be endogenously (i.e., naturally) capable of producing flea peritrophin proteins of the present invention or can be capable of producing such proteins after being transformed with at least one nucleic acid molecule of the present invention. Host cells of the present invention can be any cell capable of producing at least one protein of the present invention, and include bacterial, fungal (including yeast), parasite (including helminth, protozoa and ectoparasite), other insect, other animal and plant cells. Preferred host cells include bacterial, mycobacterial, yeast, insect and mammalian cells. More preferred host cells include Salmonella, Escherichia, Bacillus, Caulobacter, Listeria, Saccharomyces, Pichia, Spodoptera, Mycobacteria, Trichoplusia, BHK (baby hamster kidney) cells, MDCK cells (Madin-Darby canine kidney cell line), CRFK cells (Crandell feline kidney cell line), CV-1 cells (African monkey kidney cell line used, for example, to culture raccoon poxvirus), COS (e.g., COS-7) cells, and Vero cells. Particularly preferred host cells are Escherichia coli, including E. coli K-12 derivatives; Salmonella typhi; Salmonella typhimurium, including attenuated strains such as UK-1_(χ)3987 and SR-11_(χ)4072; Caulobacter; Pichia; Spodoptera frugiperda; Trichoplusia ni; BHK cells; MDCK cells; CRFK cells; CV-1 cells; COS cells; Vero cells; and non-tumorigenic mouse myoblast G8 cells (e.g., ATCC CRL 1246). Additional appropriate mammalian cell hosts include other kidney cell lines, other fibroblast cell lines (e.g., human, murine or chicken embryo fibroblast cell lines), myeloma cell lines, Chinese hamster ovary cells, mouse NIH/3T3 cells, LMTK³¹ cells and/or HeLa cells. In one embodiment, the proteins may be expressed as heterologous proteins in myeloma cell lines employing immunoglobulin promoters.

A recombinant cell is preferably produced by transforming a host cell with one or more recombinant molecules, each comprising one or more nucleic acid molecules of the present invention operatively linked to an expression vector containing one or more transcription control sequences, examples of which are disclosed herein. The phrase operatively linked refers to insertion of a nucleic acid molecule into an expression vector in a manner such that the molecule is able to be expressed when transformed into a host cell.

A recombinant cell of the present invention includes any cell transformed with at least one of any nucleic acid molecule of the present invention. Suitable and preferred nucleic acid molecules as well as suitable and preferred recombinant molecules with which to transfer cells are disclosed herein.

Recombinant cells of the present invention can also be co-transformed with one or more recombinant molecules including flea peritrophin nucleic acid molecules encoding one or more proteins of the present invention and one or more other nucleic acid molecules encoding other protective compounds, as disclosed herein (e.g., to produce multivalent vaccines).

Recombinant DNA technologies can be used to improve expression of transformed nucleic acid molecules by manipulating, for example, the number of copies of the nucleic acid molecules within a host cell, the efficiency with which those nucleic acid molecules are transcribed, the efficiency with which the resultant transcripts are translated, and the efficiency of post-translational modifications. Recombinant techniques useful for increasing the expression of nucleic acid molecules of the present invention include, but are not limited to, operatively linking nucleic acid molecules to high-copy number plasmids, integration of the nucleic acid molecules into one or more host cell chromosomes, addition of vector stability sequences to plasmids, substitutions or modifications of transcription control signals (e.g., promoters, operators, enhancers), substitutions or modifications of translational control signals (e.g., ribosome binding sites, Shine-Dalgarno sequences), modification of nucleic acid molecules of the present invention to correspond to the codon usage of the host cell, deletion of sequences that destabilize transcripts, and use of control signals that temporally separate recombinant cell growth from recombinant enzyme production during fermentation. The activity of an expressed recombinant protein of the present invention may be improved by fragmenting, modifying, or derivatizing nucleic acid molecules encoding such a protein.

Isolated flea peritrophin proteins of the present invention can be produced in a variety of ways, including production and recovery of natural proteins, production and recovery of recombinant proteins, and chemical synthesis of the proteins. In one embodiment, an isolated protein of the present invention is produced by culturing a cell capable of expressing the protein under conditions effective to produce the protein, and recovering the protein. A preferred cell to culture is a recombinant cell of the present invention. Effective culture conditions include, but are not limited to, effective media, bioreactor, temperature, pH and oxygen conditions that permit protein production. An effective, medium refers to any medium in which a cell is cultured to produce a flea peritrophin protein of the present invention. Such medium typically comprises an aqueous medium having assimilable carbon, nitrogen and phosphate sources, and appropriate salts, minerals, metals and other nutrients, such as vitamins. Cells of the present invention can be cultured in conventional fermentation bioreactors, shake flasks, test tubes, microtiter dishes, and petri plates. Culturing can be carried out at a temperature, pH and oxygen content appropriate for a recombinant cell. Such culturing conditions are within the expertise of one of ordinary skill in the art.

Depending on the vector and host system used for production, resultant proteins of the present invention may either remain within the recombinant cell; be secreted into the fermentation medium; be secreted into a space between two cellular membranes, such as the periplasmic space in E. coli; or be retained on the outer surface of a cell or viral membrane.

The phrase “recovering the protein”, as well as similar phrases, refers to collecting the whole fermentation medium containing the protein and need not imply additional steps of separation or purification. Proteins of the present invention can be purified using a variety of standard protein purification techniques, such as, but not limited to, affinity chromatography, ion exchange chromatography, filtration, electrophoresis, hydrophobic interaction chromatography, gel filtration chromatography, reverse phase chromatography, concanavalin A chromatography, chromatofocusing and differential solubilization. Proteins of the present invention are preferably retrieved in “substantially pure” form. As used herein, “substantially pure” refers to a purity that allows for the effective use of the protein as a therapeutic composition or diagnostic. A therapeutic composition for animals, for example, should exhibit no substantial toxicity and preferably should be capable of stimulating the production of antibodies in a treated animal.

The present invention also includes isolated (i.e., removed from their natural milieu) antibodies that selectively bind to a flea peritrophin protein of the present invention or a mimetope thereof (e.g., anti-flea peritrophin antibodies). As used herein, the term “selectively binds to” a protein refers to the ability of antibodies of the present invention to preferentially bind to specified proteins and mimetopes thereof of the present invention. Binding can be measured using a variety of methods standard in the art including enzyme immunoassays (e.g., ELISA), immunoblot assays, etc.; see, for example, Sambrook et al., ibid., and Harlow, et al., 1988, Antibodies, a Laboratory Manual, Cold Spring Harbor Labs Press; Harlow et al., ibid., is incorporated by reference herein in its entirety. An anti-flea peritrophin antibody of the present invention preferably selectively binds to a flea peritrophin protein, respectively, in such a way as to inhibit the function of that protein.

Isolated antibodies of the present invention can include antibodies in serum, or antibodies that have been purified to varying degrees. Antibodies of the present invention can be polyclonal or monoclonal, or can be functional equivalents such as antibody fragments and genetically-engineered antibodies, including single chain antibodies or chimeric antibodies that can bind to one or more epitopes.

A preferred method to produce antibodies of the present invention includes (a) administering to an animal an effective amount of a protein, peptide or mimetope thereof of the present invention to produce the antibodies and (b) recovering the antibodies. In another method, antibodies of the present invention are produced recombinantly using techniques as heretofore disclosed to produce flea peritrophin proteins of the present invention. Antibodies raised against defined proteins or mimetopes can be advantageous because such antibodies are not substantially contaminated with antibodies against other substances that might otherwise cause interference in a diagnostic assay or side effects if used in a therapeutic composition.

Antibodies of the present invention have a variety of potential uses that are within the scope of the present invention. For example, such antibodies can be used (a) as therapeutic compounds to passively immunize an animal in order to protect the animal from fleas susceptible to treatment by such antibodies and/or (b) as tools to screen expression libraries and/or to recover desired proteins of the present invention from a mixture of proteins and other contaminants. Furthermore, antibodies of the present invention can be used to target cytotoxic agents to fleas in order to directly kill such fleas. Targeting can be accomplished by conjugating (i.e., stably joining) such antibodies to the cytotoxic agents using techniques known to those skilled in the art. Suitable cytotoxic agents are known to those skilled in the art.

One embodiment of the present invention is a therapeutic composition that, when administered to an animal susceptible to flea infestation, is capable of protecting that animal from flea infestation. Therapeutic compositions of the present invention include at least one of the following protective molecules: an isolated flea peritrophin protein; a mimetope of an isolated flea peritrophin protein; an isolated flea peritrophin nucleic acid molecule; and/or a compound derived from said isolated flea peritrophin protein that inhibits flea peritrophin protein activity. A therapeutic composition of the present invention can further comprise a component selected from the group of an excipient, a carrier, and/or an adjuvant; these components are described further herein. As used herein, a protective molecule or protective compound refers to a compound that, when administered to an animal in an effective manner, is able to treat, ameliorate, and/or prevent flea infestation. Preferred fleas to target are heretofore disclosed. One example of a protective molecule is a vaccine, such as, but not limited to, a naked nucleic acid vaccine, a recombinant virus vaccine, a recombinant cell vaccine, and a recombinant protein vaccine. Another example of a protective molecule is a compound that inhibits flea peritrophin protein activity, such as an isolated antibody that selectively binds to a flea peritrophin protein, a substrate analog of a flea peritrophin protein, anti-sense-, triplex formation-, ribozyme-, and/or RNA drug-based compounds, or other inorganic or organic molecules that inhibit flea peritrophin protein activity. Inhibiting flea peritrophin protein activity can refer to the ability of a compound to reduce the activity of flea peritrophin proteins. Inhibiting flea peritrophin protein activity can also refer to the ability of a compound to reduce the amount of flea peritrophin protein in a flea.

One embodiment of the present invention is a therapeutic composition comprising an excipient and a compound selected from the group consisting of: (a) an isolated nucleic acid molecule selected from the group consisting of a flea cDNA molecule and a flea RNA molecule, wherein said nucleic acid molecule is selected from the group consisting of (a) a nucleic acid molecule at least 25 nucleotides in length that hybridizes with a nucleic acid molecule having a nucleic acid sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, and SEQ ID NO:32, and (b) a nucleic acid molecule at least 35 nucleotides in length that hybridizes with a nucleic acid molecule having a nucleic acid sequence selected from the group consisting of SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:44, SEQ ID NO:45, SEQ ID NO:46, SEQ ID NO:47, and SEQ ID NO:49; wherein said hybridization of (a) and (b) is performed under conditions comprising (1) hybridizing in a solution comprising 1×SSC in the absence of nucleic acid helix destabilizing compounds, at a temperature of 37° C. and (2) washing in a solution comprising 1×SSC in the absence of helix destabilizing compounds, at a temperature of 47° C.; (b) an isolated protein selected from the group consisting of (1) a protein encoded by a nucleic acid molecule selected from the group consisting of (i) a nucleic acid molecule at least 25 nucleotides in length that hybridizes with a nucleic acid molecule having a nucleic acid sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, and SEQ ID NO:32, and (ii) a nucleic acid molecule at least 35 nucleotides in length that hybridizes with a nucleic acid molecule having a nucleic acid sequence selected from the group consisting of SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:44, SEQ ID NO:45, SEQ ID NO:46, SEQ ID NO:47, and SEQ ID NO:49; wherein said hybridization of (i) and (ii) is performed under conditions comprising (a) hybridizing in a solution comprising 1×SSC in the absence of nucleic acid helix destabilizing compounds, at a temperature of 37° C. and (b) washing in a solution comprising 1×SSC in the absence of helix destabilizing compounds, at a temperature of 47° C.; and (2) an isolated protein, comprising at least 10 amino acids identical in sequence to a 10 amino acid portion of an amino acid sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:12, SEQ ID NO:17, SEQ ID NO:26, SEQ ID NO:31, SEQ ID NO:40, SEQ ID NO:43, and SEQ ID NO:48; and (c) an isolated antibody that selectively binds to a protein as set forth in (b).

Another embodiment of the present invention includes a method to reduce flea infestation in an animal susceptible to flea infestation. Such a method includes the step of administering to the animal a therapeutic molecule comprising a protective compound selected from the group consisting of (a) an isolated flea peritrophin protein; (b) a mimetope of an isolated flea peritrophin protein; (c) an isolated flea peritrophin nucleic acid molecule; and (d) a compound derived from an isolated flea peritrophin protein that inhibits flea peritrophin protein activity.

Therapeutic compositions of the present invention can be administered to any animal susceptible to flea infestation, preferably to mammals, and more preferably to dogs, cats, humans, ferrets, horses, cattle, sheep, and other pets, economic food animals, work animals and/or zoo animals. Preferred animals to protect against flea infestation include dogs, cats, humans, and ferrets, with dogs and cats being particularly preferred.

As used herein, the term derived, or the term derived from, refers to a peptide, antibody, mimetope, nucleic acid molecule, or other compound that was obtained from or using a flea peritrophin protein or nucleic acid molecule of the present invention. Methods to obtain derivatives from a flea peritrophin molecule of the present invention are known in the art, and as such include, but are not limited to molecular modeling of flea peritrophin proteins to determine active sites, and predicting from these active sites smaller fragments and/or mimetopes that retain and/or mimic these active sites, thereby inhibiting flea peritrophin protein activity. Other inhibitors of flea peritrophin activity can also be obtained in a variety of ways, including but not limited to screening of peptide or small chemical compound libraries against flea peritrophin proteins of the present invention; and screening of polyclonal or monoclonal antibodies to find antibodies that specifically bind flea peritrophin proteins of the present invention.

A flea peritrophin protein inhibitor of the present invention (i.e. an inhibitor of a flea peritrophin protein) is identified by its ability to mimic, bind to, modify, or otherwise interact with, a flea peritrophin protein, thereby inhibiting the activity of a natural flea peritrophin protein. Suitable inhibitors of flea peritrophin protein activity are compounds that inhibit flea peritrophin protein activity in at least one of a variety of ways: (a) by binding to or otherwise interacting with or otherwise modifying flea peritrophin protein sites; (b) by binding to the flea peritrophin protein and thus reducing the availability of the flea peritrophin protein in solution; (c) by mimicking a flea peritrophin protein; and (d) by interacting with other regions of the flea peritrophin protein to inhibit flea peritrophin protein activity, for example, by allosteric interaction.

Flea peritrophin protein inhibitors can be used directly as compounds in compositions of the present invention to treat animals as long as such compounds are not harmful to host animals being treated. Preferred flea peritrophin protein inhibitors of the present invention include, but are not limited to, flea peritrophin protein substrate analogs, and other molecules that bind to a flea peritrophin protein (e.g., to an allosteric site) in such a manner that the activity of the flea peritrophin protein is inhibited. A flea peritrophin protein substrate analog refers to a compound that interacts with (e.g., binds to, associates with, modifies) the active site of a flea peritrophin protein. A preferred flea peritrophin protein substrate analog inhibits flea peritrophin protein activity. Flea peritrophin protein substrate analogs can be of any inorganic or organic composition. Flea peritrophin protein substrate analogs can be, but need not be, structurally similar to a flea peritrophin protein natural substrate as long as they can interact with the active site of that flea peritrophin protein. Flea peritrophin protein substrate analogs can be designed using computer-generated structures of flea peritrophin proteins of the present invention or computer structures of flea peritrophin protein's natural substrates. Preferred sites to model include one or more of the active sites of flea peritrophin proteins. Substrate analogs can also be obtained by generating random samples of molecules, such as oligonucleotides, peptides, peptidomimetic compounds, or other inorganic or organic molecules, and screening such samples for their ability to interfere with interaction between flea peritrophin proteins and their substrates, e.g. by affinity chromatography techniques. A preferred flea peritrophin protein substrate analog is a flea peritrophin protein mimetic compound, i.e., a compound that is structurally and/or functionally similar to a natural substrate of a flea peritrophin protein of the present invention, particularly to the region of the substrate that interacts with the flea peritrophin protein active site, but that inhibits flea peritrophin protein activity upon interacting with the flea peritrophin protein active site.

The present invention also includes a therapeutic composition comprising at least one protective molecule of the present invention in combination with at least one additional compound protective against one or more infectious agents.

In one embodiment, a therapeutic composition of the present invention can be used to protect an animal from flea infestation by administering such composition to a flea in order to prevent infestation. Such administration to the flea and/or animal could be oral, or by application to the animal's body surface (e.g. topical spot-on, or spraying onto the animal), or by application to the environment (e.g., spraying). Examples of such compositions include, but are not limited to, transgenic vectors capable of producing at least one therapeutic composition of the present invention. In another embodiment a flea can ingest therapeutic compositions, or products thereof, present on the surface of or in the blood of a host animal that has been administered a therapeutic composition of the present invention.

In accordance with the present invention, a host animal (i.e., an animal that is or is capable of being infested with fleas) is treated by administering to the animal a therapeutic composition of the present invention in such a manner that the composition itself (e.g., a flea peritrophin protein, a flea peritrophin nucleic acid molecule, a flea peritrophin protein inhibitor, a peritrophin protein synthesis suppressor (i.e., a compound that decreases the production or half-life of a peritrophin protein in fleas), a flea peritrophin protein mimetope, or a anti-flea peritrophin antibody) or a product generated by the animal in response to administration of the composition (e.g., antibodies produced in response to administration of a flea peritrophin protein or nucleic acid molecule, or conversion of an inactive inhibitor “prodrug” to an active flea peritrophin protein inhibitor) ultimately enters the flea. A host animal is preferably treated in such a way that the compound or product thereof is present on the body surface of the animal or enters the blood stream of the animal. Fleas are then exposed to the composition or product when they feed from the animal. For example, flea peritrophin protein inhibitors administered to an animal are administered in such a way that the inhibitors enter the blood stream of the animal, where they can be taken up by feeding fleas.

The present invention also includes the ability to reduce larval flea infestation in that when fleas feed from a host animal that has been administered a therapeutic composition of the present invention, at least a portion of compounds of the present invention, or products thereof, in the blood taken up by the fleas are excreted by the fleas in feces, which is subsequently ingested by flea larvae. In particular, it is of note that flea larvae obtain most, if not all, of their nutrition from flea feces.

In accordance with the present invention, reducing flea peritrophin protein activity in a flea can lead to a number of outcomes that reduce flea burden on treated animals and their surrounding environments. Such outcomes include, but are not limited to, (a) reducing the viability of fleas that feed from the treated animal, (b) reducing the fecundity of female fleas that feed from the treated animal, (c) reducing the reproductive capacity of male fleas that feed from the treated animal, (d) reducing the viability of eggs laid by female fleas that feed from the treated animal, (e) altering the blood feeding behavior of fleas that feed from the treated animal (e.g., fleas take up less volume per feeding or feed less frequently), (f) reducing the viability of flea larvae, for example due to the feeding of larvae from feces of fleas that feed from the treated animal, (g) altering the development of flea larvae (e.g., by decreasing feeding behavior, inhibiting growth, inhibiting (e.g., slowing or blocking) molting, and/or otherwise inhibiting maturation to adults), and/or (h) altering or decreasing the ability of fleas or flea larvae to digest a blood meal.

In order to protect an animal from flea infestation, a therapeutic composition of the present invention is administered to the animal in an effective manner such that the composition is capable of protecting that animal from flea infestation. Therapeutic compositions of the present invention can be administered to animals prior to infestation in order to prevent infestation (i.e., as a preventative vaccine) and/or can be administered to animals after infestation. For example, proteins, mimetopes thereof, and antibodies thereof can be used as immunotherapeutic agents.

Therapeutic compositions of the present invention can be formulated in an excipient that the animal to be treated can tolerate. Examples of such excipients include water, saline, Ringer's solution, dextrose solution, Hank's solution, and other aqueous physiologically balanced salt solutions. Nonaqueous vehicles, such as fixed oils, sesame oil, ethyl oleate, or triglycerides may also be used. Other useful formulations include suspensions containing viscosity enhancing agents, such as sodium carboxymethylcellulose, sorbitol, or dextran. Excipients can also contain minor amounts of additives, such as substances that enhance isotonicity and chemical stability. Examples of buffers include phosphate buffer, bicarbonate buffer and Tris buffer, while examples of preservatives include thimerosal, or o-cresol, formalin and benzyl alcohol. Standard formulations can either be liquid injectables or solids which can be taken up in a suitable liquid as a suspension or solution for injection. Thus, in a non-liquid formulation, the excipient can comprise dextrose, serum albumin, preservatives, etc., to which sterile water or saline can be added prior to administration.

In one embodiment of the present invention, a therapeutic composition can include an adjuvant. Adjuvants are agents that are capable of enhancing the immune response of an animal to a specific antigen. Suitable adjuvants include, but are not limited to, cytokines, chemokines, and compounds that induce the production of cytokines and chemokines (e.g., granulocyte macrophage colony stimulating factor (GM-CSF), Flt-3 ligand, granulocyte colony stimulating factor (G-CSF), macrophage colony stimulating factor (M-CSF), colony stimulating factor (CSF), erythropoietin (EPO), interleukin 2 (IL-2), interleukin-3 (IL-3), interleukin 4 (IL-4), interleukin 5 (IL-5), interleukin 6 (IL-6), interleukin 7 (IL-7), interleukin 8 (IL-8), interleukin 10 (IL-10), interleukin 12 (IL-12), interferon gamma, interferon gamma inducing factor I (IGIF), transforming growth factor beta, RANTES (regulated upon activation, normal T cell expressed and presumably secreted), macrophage inflammatory proteins (e.g., MIP-1 alpha and MIP-1 beta), and Leishmania elongation initiating factor (LEIF)); bacterial components (e.g., endotoxins, in particular superantigens, exotoxins and cell wall components); aluminum-based salts; calcium-based salts; silica; polynucleotides; toxoids; serum proteins, viral coat proteins; block copolymer adjuvants (e.g., Hunter's Titermax™ adjuvant (Vaxcel™, Inc. Norcross, Ga.), Ribi adjuvants (Ribi ImmunoChem Research, Inc., Hamilton, Mont.); and saponins and their derivatives (e.g., Quil A (Superfos Biosector A/S, Denmark). Protein adjuvants of the present invention can be delivered in the form of the protein themselves or of nucleic acid molecules encoding such proteins using the methods described herein.

In one embodiment of the present invention, a therapeutic composition can include a carrier. Carriers include compounds that increase the half-life of a therapeutic composition in the treated animal. Suitable carriers include, but are not limited to, polymeric controlled release vehicles, biodegradable implants, liposomes, bacteria, viruses, other cells, oils, esters, and glycols.

One embodiment of the present invention is a controlled release formulation that is capable of slowly releasing a composition of the present invention into an animal. As used herein, a controlled release formulation comprises a composition of the present invention in a controlled release vehicle. Suitable controlled release vehicles include, but are not limited to, biocompatible polymers, other polymeric matrices, capsules, microcapsules, microparticles, bolus preparations, osmotic pumps, diffusion devices, liposomes, lipospheres, and transdermal delivery systems. Other controlled release formulations of the present invention include liquids that, upon administration to an animal, form a solid or a gel in situ. Preferred controlled release formulations are biodegradable (i.e., bioerodible).

A preferred controlled release formulation of the present invention is capable of releasing a composition of the present invention into the blood of the treated animal at a constant rate sufficient to attain therapeutic dose levels of the composition. The therapeutic composition is preferably released over a period of time ranging from 1 to 12 months. A controlled release formulation of the present invention is capable of effecting a treatment preferably for at least 1 month, preferably for at least 3 months, preferably for at least 6 months, preferably for at least 9 months, and preferably for at least 12 months.

Acceptable protocols to administer therapeutic compositions in an effective manner include individual dose size, number of doses, frequency of dose administration, and mode of administration. Determination of such protocols can be accomplished by those skilled in the art. A suitable single dose is a dose that is capable of treating an animal when administered one or more times over a suitable time period. For example, a preferred single dose of an inhibitor is from 1 microgram (μg) to 10 milligrams (mg) of the therapeutic composition per kilogram body weight of the animal. Booster vaccinations can be administered from 2 weeks to several years after the original administration. Booster administrations preferably are administered when the immune response of the animal becomes insufficient to protect the animal from disease. A preferred administration schedule is one in which from 10 μg to 1 mg of the therapeutic composition per kg body weight of the animal is administered from one to two times over a time period of from 2 weeks to 12 months. Modes of administration can include, but are not limited to, subcutaneous, intradermal, intravenous, intranasal, oral, transdermal, intraocular, intranasal, conjunctival, and intramuscular routes. Methods of administration for other therapeutic compounds can be determined by one skilled in the art, and may include administration of a therapeutic composition one or more times, on a daily, weekly, monthly or yearly regimen; routes of administration can be determined by one skilled in the art, and may include any route. A preferred route of administration of an inhibitory compound when administering to fleas is a topical, or “spot-on” formulation administered to the body surface of the animal, so that a flea would encounter the inhibitory compound when attached to the animal; another preferred route of administration of an inhibitory compound is an oral formulation that, when fed to an animal, would enter the bloodstream of the animal, which would then be transferred to a flea while feeding from the animal.

A recombinant protein vaccine of the present invention comprises a recombinantly-produced flea peritrophin protein of the present invention that is administered to an animal according to a protocol that results in the animal producing a sufficient immune response to protect itself from a flea infestation. Such protocols can be determined by those skilled in the art.

According to one embodiment, a nucleic acid molecule of the present invention can be administered to an animal in a fashion to enable expression of that nucleic acid molecule into a protective protein or protective RNA (e.g., antisense RNA, ribozyme, triple helix forms or RNA drug) in the animal. Nucleic acid molecules can be delivered to an animal in a variety of methods including, but not limited to, (a) administering a naked (i.e., not packaged in a viral coat or cellular membrane) nucleic acid as a genetic vaccine (e.g., as naked DNA or RNA molecules, such as is taught, for example in Wolff et al., 1990, Science 247, 1465-1468) or (b) administering a nucleic acid molecule packaged as a recombinant virus vaccine or as a recombinant cell vaccine (i.e., the nucleic acid molecule is delivered by a viral or cellular vehicle).

A genetic (i.e., naked nucleic acid) vaccine of the present invention includes a nucleic acid molecule of the present invention and preferably includes a recombinant molecule of the present invention that preferably is replication, or otherwise amplification, competent. A genetic vaccine of the present invention can comprise one or more nucleic acid molecules of the present invention in the form of, for example, a dicistronic recombinant molecule. Preferred genetic vaccines include at least a portion of a viral genome, i.e., a viral vector. Preferred viral vectors include those based on alphaviruses, poxviruses, adenoviruses, herpesviruses, picornaviruses, and retroviruses, with those based on alphaviruses, such as sindbis or Semliki forest virus, species-specific herpesviruses and poxviruses being particularly preferred. Any suitable transcription control sequence can be used, including those disclosed as suitable for protein production. Particularly preferred transcription control sequences include cytomegalovirus immediate early (preferably in conjunction with Intron-A), Rous sarcoma virus long terminal repeat, and tissue-specific transcription control sequences, as well as transcription control sequences endogenous to viral vectors if viral vectors are used. The incorporation of a “strong” polyadenylation signal is also preferred.

Genetic vaccines of the present invention can be administered in a variety of ways, with intramuscular, subcutaneous, intradermal, transdermal, conjunctival, intraocular, intranasal and oral routes of administration being preferred. A preferred single dose of a genetic vaccine ranges from 1 nanogram (ng) to 600 μg, depending on the route of administration and/or method of delivery, as can be determined by those skilled in the art. Suitable delivery methods include, for example, by injection, as drops, aerosolized and/or topically. Genetic vaccines of the present invention can be contained in an aqueous excipient (e.g., phosphate buffered saline) alone or in a carrier (e.g., lipid-based vehicles).

A recombinant virus vaccine of the present invention includes a recombinant molecule of the present invention that is packaged in a viral coat and that can be expressed in an animal after administration. Preferably, the recombinant molecule is packaging- or replication-deficient and/or encodes an attenuated virus. A number of recombinant viruses can be used, including, but not limited to, those based on alphaviruses, poxviruses, adenoviruses, herpesviruses, picornaviruses, and retroviruses. Preferred recombinant virus vaccines are those based on alphaviruses (such as Sindbis virus), raccoon poxviruses, species-specific herpesviruses and species-specific poxviruses. An example of methods to produce and use alphavirus recombinant virus vaccines are disclosed in U.S. Pat. No. 5,766,602 to Xiong and Grieve, which is incorporated by reference herein in its entirety.

When administered to an animal, a recombinant virus vaccine of the present invention infects cells within the immunized animal and directs the production of a protective protein or RNA nucleic acid molecule that is capable of protecting the animal from flea infestation as disclosed herein. For example, a recombinant virus vaccine comprising a flea peritrophin nucleic acid molecule of the present invention is administered according to a protocol that results in the animal producing a sufficient immune response to protect itself from flea infestation. A preferred single dose of a recombinant virus vaccine of the present invention is from 1×10⁴ to 1×10⁸ virus plaque forming units (pfu) per kilogram body weight of the animal. Administration protocols are similar to those described herein for protein-based vaccines, with subcutaneous, intramuscular, intranasal, intraocular, conjunctival, and oral administration routes being preferred.

A recombinant cell vaccine of the present invention includes recombinant cells of the present invention that express at least one protein of the present invention. Preferred recombinant cells for this embodiment include Salmonella, E. coli, Listeria, Mycobacterium, S. frugiperda, yeast, (including Saccharomyces cerevisiae and Pichia pastoris), BHK, CV-1, myoblast G8, COS (e.g., COS-7), Vero, MDCK and CRFK recombinant cells. Recombinant cell vaccines of the present invention can be administered in a variety of ways but have the advantage that they can be administered orally, preferably at doses ranging from 10⁸ to 10¹² cells per kilogram body weight. Administration protocols are similar to those described herein for protein-based vaccines. Recombinant cell vaccines can comprise whole cells, cells stripped of cell walls or cell lysates.

The efficacy of a therapeutic composition of the present invention to protect an animal from flea infestation can be tested in a variety of ways including, but not limited to, detection of protective antibodies (using, for example, proteins or mimetopes of the present invention), detection of cellular immunity within the treated animal, or challenge of the treated animal with the fleas to determine whether the treated animal is resistant to infestation. Challenge studies can include direct administration of fleas to the treated animal. In one embodiment, therapeutic compositions can be tested in animal models such as mice. Such techniques are known to those skilled in the art.

As discussed herein, one therapeutic composition of the present invention includes an inhibitor of flea peritrophin protein activity, i.e., a compound capable of substantially interfering with the function of a flea peritrophin protein. An inhibitor of flea peritrophin protein activity, or function, can be identified using flea peritrophin proteins of the present invention. A preferred inhibitor of flea peritrophin protein function is a compound capable of substantially interfering with the function of a flea peritrophin protein and which does not substantially interfere with the function of host animal peritrophin proteins. As used herein, a compound that does not substantially inhibit or interfere with host animal peritrophin proteins is one that, when administered to a host animal, the host animal shows no significant adverse effects attributable to the inhibition of peritrophin and which, when administered to an animal in an effective manner, is capable of protecting that animal from flea infestation.

One embodiment of the present invention is a method to identify a compound capable of inhibiting flea peritrophin protein activity. Such a method includes the steps of (a) contacting (e.g., combining, mixing) an isolated flea peritrophin protein of the present invention, with a putative inhibitory compound under conditions in which, in the absence of the compound, the protein has flea peritrophin protein activity, and (b) determining if the putative inhibitory compound inhibits the activity. Such conditions under which a flea peritrophin protein has flea peritrophin protein activity include conditions in which a flea peritrophin protein has a correct three-dimensionally folded structure under physiologic conditions, i.e. physiologic pH, physiologic ionic concentrations, and physiologic temperatures. Putative inhibitory compounds to screen include antibodies (including fragments and mimetopes thereof), putative substrate analogs, and other, preferably small, organic or inorganic molecules.

A preferred method to identify a compound capable of inhibiting flea peritrophin protein activity includes contacting an isolated flea peritrophin protein of the present invention with a putative inhibitory compound under conditions in which, in the absence of the compound, the protein has flea peritrophin protein activity; and determining if the putative inhibitory compound inhibits the activity.

Another embodiment of the present invention is an assay kit to identify an inhibitor of a flea peritrophin protein of the present invention. This kit comprises an isolated flea peritrophin protein of the present invention, and a means for determining inhibition of an activity of flea peritrophin protein, where the means enables detection of inhibition. Detection of inhibition of flea peritrophin protein identifies a putative inhibitor to be an inhibitor of a flea peritrophin protein. Means for determining inhibition of a flea peritrophin protein include, for example, an assay system that detects binding of a putative inhibitor to a flea peritrophin molecule, and an assay system that detects interference by a putative inhibitor of the ability of flea peritrophin protein to hydrolyze a substrate. Means and methods are described herein and are known to those skilled in the art.

The following examples are provided for the purposes of illustration and are not intended to limit the scope of the present invention. The following examples include a number of recombinant DNA and protein chemistry techniques known to those skilled in the art; see, for example, Sambrook et al., ibid.

EXAMPLE 1

This Example describes the isolation of RNA from the hindgut and Malpighian tubules (HMT) of Ctenocephalides felis and the use of isolated RNA to construct subtracted and unsubtracted cDNA libraries.

Approximately 10,000 hindguts and Malpighian tubules were dissected from equal numbers of cat blood fed and unfed adult C. felis with a male to female ratio of 1 to 4, and total RNA was extracted using a guanidine isothiocyanate lysis buffer and the standard procedure described by Sambrook et al. Poly-A enriched mRNA was purified from total RNA above using a mRNA Purification Kit, available from Pharmacia Biotech, Piscataway, N.J., following the manufacturer's protocol. The same procedures were used to extract total RNA and isolate poly-A enriched mRNA from the dissected C. felis bodies following removal of HMT, referred to hereinafter as “non-HMT mRNA”.

Poly-A enriched mRNA was used to construct a cDNA library using subtractive hybridization and suppression PCR as follows. Subtractive hybridization and suppression PCR was conducted using a PCR-Select™ cDNA Subtraction Kit, available from Clontech Laboratories, Inc., Palo Alto, Calif. according to the manufacturer's instructions. Briefly, this kit uses subtractive hybridization and suppression PCR to specifically amplify cDNA sequences that are present in the tester cDNA and absent in the driver cDNA, thus enriching for tester-specific sequences. The efficiency of the subtraction process can be assessed by semi-quantitative PCR and by comparing the ethidium bromide staining patterns of the subtracted and unsubtracted samples on agarose gels as described in section V.D. of the manufacturer's protocol. For the semi-quantitative PCR, three genes with mRNAs known to be expressed outside of the HMT tissue were used to test for specific subtraction. These genes encoded putative actin, N-aminopeptidase, and serine protease proteins.

Subtractive hybridization and suppression PCR was conducted under the following conditions. Two micrograms (μg) of HMT mRNA was used as the template for synthesis of the tester material and 2 μg of non-HMT mRNA was used as template for synthesis of the driver material in this reaction. The number of cycles used in the selective amplification steps was optimized using the manufacturer's protocols. Optimization resulted in the use of 24 rather than the standard 27 cycles of primary PCR in combination with 15 cycles of secondary PCR rather than the standard 12 cycles.

The products from the suppressive PCR reaction were ligated into the pCR®2.1 vector, available from Invitrogen, Carlsbad, Calif., using an Original TA Cloning® Kit, available from Invitrogen. The ligation reaction was then used to transform INVαF′ One Shot™ competent cells, available from Invitrogen, which were plated on Luria broth (LB) agar with 50 micrograms per milliliter (μg/ml) ampicillin, available from Sigma-Aldrich Co., St. Louis, Mo., and 50 μg/ml 5-bromo-4-chloro-3-indoyl β-D-galactopyranoside (X-Gal), available from Fisher Biotech, Fair Lawn, N.J. Transformed colonies were amplified and the DNA isolated using the standard alkaline lysis procedure described by Sambrook et al., ibid.

Automated cycle sequencing of DNA samples was performed using an ABI PRISM™ Model 377, available from Perkins Elmer, with XL upgrade DNA Sequencer, available from PE Applied Biosystems, Foster City, Calif., after reactions were carried out using the PRISM™ Dye Terminator Cycle Sequencing Ready Reaction Kit or the PRISM™ dRhodamine Terminator Cycle Sequencing Ready Reaction Kit or the PRISM™ BigDye™ Terminator Cycle sequencing Ready Reaction Kit, available from PE Applied Biosystems, following the manufacturer's protocol, hereinafter “standard sequencing methods”. Sequence analysis was performed using SeqLab, using default parameters. Each sequence read was trimmed of vector sequence at either end and submitted for a search through the National Center for Biotechnology Information (NCBI), National Library of Medicine, National Institute of Health, Baltimore, Md., using the BLAST network. This database includes SwissProt+PIR+SPupdate+GenPept+GPUpdate+PDB databases. The search was conducted using the xBLAST function, which compares the translated sequences in all 6 reading frames to the protein sequences contained in the database.

An unsubtracted HMT cDNA library was constructed as follows. Approximately 10,000 HMT tissues were dissected from equal numbers of unfed and cat blood-fed adult C. felis with a male to female ratio of 1:4. Total RNA was extracted using a guanidine isothiocyanate lysis buffer and procedures described in Sambrook et al., followed by isolation using a mRNA purification kit, available from Pharmacia, according to the manufacturer's protocols. The library was constructed with 5 μg of isolated mRNA using a ZAP-cDNA® cDNA synthesis kit, and packaged using a ZAP-cDNA® Gigapack® gold cloning kit, both available from Stratagene, La Jolla, Calif. The resultant HMT library was amplified to a titer of 5×10⁹ plaque forming units per milliliter (pfu/ml). Single clone excisions were performed using the Ex-Assist™ helper phage, available from Stratagene, and used to create double stranded plasmid template for sequencing using the manufacturer's protocols with the following exceptions. Following incubation of the SOLR cells with the cleared phage lysate, the mixture was used to inoculate LB broth, and the mix was incubated overnight and then subjected to mini-prep plasmid preparation and sequencing as described for the subtracted HMT library above.

EXAMPLE 2

This example describes the production of a C. felis cDNA pool by Rapid Amplification of cDNA Ends (RACE cDNA pool).

Total RNA was extracted from adult fed and unfed fleas as follows. Approximately 1000 adult fed fleas and 1000 adult unfed fleas were frozen on dry ice and separately ground into powder using a mortar and pestle and total RNA was extracted from each powder as follows. Ten ml of solution D (4 M guanidine isothiocyanate, 25 mM Sodium Citrate pH 7.0, 1.5% Sarcosyl, 0.5 M 2-mercaptoethanol) were added to the powder and the suspension was mixed by shaking. One ml of 2M sodium acetate, pH 4.0 and 3 ml of pH 4.7 phenol/chloroform/isoamyl alcohol (125:24:1), available from Sigma, were added and the suspension was mixed on a vortex shaker then incubated on ice for 15 minutes. Following incubation, the mixture was centrifuged at 10,000×g for 20 minutes and the supernatant was removed and extracted twice with pH 4.7 phenol/chloroform/isoamyl alcohol. Next, an equal volume of isopropanol was added to the supernatant and incubated at −20° C. for 2 hours followed by centrifugation at 10,000×g for 20 minutes. Following centrifugation, the supernatant was removed and discarded and the pellet was washed in 70% ethanol and allowed to dry at room temperature. The pellet was resuspended in 10 mM Tris 1 mM EDTA pH 8.0. Spectrophotometer analysis indicated that the yield of total RNA from unfed fleas was 1140 μg and the yield from fed fleas was 1500 μg.

Six-hundred pg from each of the fed and unfed adult flea total RNA extractions were combined and mRNA was then extracted using a mRNA Purification Kit, available from Amersham Pharmacia Biotech, Piscataway, N.J., using the manufacture's protocol. Approximately 15-25 μg of mRNA were isolated based on spectrophotometer analysis and ethidium bromide staining. One μg of purified mRNA was used as template to construct a RACE cDNA pool using a SMART™ RACE cDNA Amplification Kit, available from Clontech Laboratories, Inc., Palo Alto, Calif., according to the manufacture's instructions.

EXAMPLE 3

This example describes the cloning, sequencing, recombinant protein expression and purification of a C. felis peritrophin-like nucleic acid molecule, referred to herein as PL1. This example also describes the expression of PL1 mRNA in a variety of flea tissues.

A. Isolation of PL1 Nucleic Acid Molecules

A TA clone from the HMT EST library described in Example 1 was sequenced using standard sequencing methods and shown to have homology to a chitinase-like gene from Bombyx mori (silkworm). This clone was digested with EcoRI to excise an insert 429 nucleotides in length, referred to as peritrophin-like molecule 1 (PL1) nucleic acid molecule nCfPL1₄₂₉. The insert was isolated by gel purification using a Gel Purification kit, available from Qiagen, Valencia, Calif. Approximately 50 ng of purified nCfPL1₄₂₉ was used to construct a ³²P α-dATP labeled DNA probe using a Megaprime DNA labeling kit, available from Amersham, using the manufacturer's protocols.

The ³²P α-dATP labeled probe was used in a plaque lift hybridization procedure to isolate a clone from the HMT lambda-ZAP unsubtracted cDNA library described in Example 1 as follows. Filters were hybridized with 1×10⁶ counts per minute (cpm) per ml of the probe in 5×SSPE, (see Sambrook et al., ibid.), 1.2% sodium dodecyl sulfate (SDS), 0.1 mg/ml salmon sperm DNA and 5×Denhardt's reagent, (see Sambrook et al., ibid.), at 55° C. for 14 hours. The filters were washed as follows: (a) 10 minutes with 5×SSPE and 1% SDS, (b) 10 minutes with 2×SSPE and 1% SDS, (c) 10 minutes with 1×SSPE and 0.5% SDS, and (d) 10 minutes with 0.5×SSPE and 1% SDS. All washes were conducted at 55° C. Plaques that hybridized strongly to the probe were isolated and subjected to in vivo excision. In vivo excision was performed using the Stratagene Ex-Assist™ helper phage system and protocols, to convert a positive plaque to pBluescript™ plasmid DNA. Sequencing was conducted using standard sequencing methods following preparation of DNA with a Qiagen Qiaprep™ spin mini prep kit using the manufacturer's instructions and restriction enzyme digestion with 1 μl of 20 U/μl each of EcoRI and XhoI, available from New England Biolabs, Beverly, Mass. Plaques that hybridized strongly to the probe were isolated and subjected to in vivo excision. In vivo excision was performed using the Stratagene Ex-Assist™ helper phage system and protocols, to convert a positive plaque to pBluescript™ plasmid DNA, and sequencing was conducted following preparation of DNA with a Qiagen Qiaprep™ spin mini prep kit using the manufacturer's instructions and restriction enzyme digestion with 1 μl of 20 U/μl each of EcoRI and XhoI, available from New England Biolabs. A clone was isolated from a primary plaque, containing a nucleic acid molecule of 1096 base pairs, referred to herein as nCfPL1₁₀₉₆, the coding strand of which has a nucleotide sequence denoted herein as SEQ ID NO:1. The complement of SEQ ID NO:1 is represented herein as SEQ ID NO:3. Sequencing of nCfPL1₄₂₉ indicated that nCfPL1₄₂₉ shares 100% identity with nucleotides 148 through 576 of SEQ ID NO:1.

Translation of SEQ ID NO:1 suggests that nucleic acid molecule nCfPL1₁₀₉₆ encodes a full-length chitin-binding protein of 272 amino acids, referred to herein as PCfCfPL1₂₇₂, having an amino acid sequence represented by SEQ ID NO:2, assuming the initiation codon spans from nucleotide 6 through nucleotide 8 of SEQ ID NO:1 and the termination codon spans from nucleotide 822 through nucleotide 824 of SEQ ID NO:1. The coding region encoding PCfPL1₂₇₂, is represented by nucleic acid molecule nCfPL1₈₁₆, having a coding strand with the nucleic acid sequence represented by SEQ ID NO:4 and a complementary strand with nucleic acid sequence represented by SEQ ID NO:5. The amino acid sequence of PCfPL1₂₇₂ predicts that PCfPL1₂₇₂ has an estimated molecular weight of 30.6 kDa and an estimated pI of 7.3.

Comparison of amino acid sequence SEQ ID NO:2 with amino acid sequences reported in GenBank indicates that SEQ ID NO:2 showed the most homology, i.e., 26% identity, with a Lucilia cuprina peritrophin-44 protein, GenBank Accession No. 407976. Comparison of SEQ ID NO:4 with nucleic acid sequences reported in GenBank indicates that SEQ ID NO:4 showed the most homology, i.e., 40%, with a Lucilia cuprina peritrophin-44 nucleic acid molecule, GenBank Accession number L25106. Percent identity calculations were performed using the SeqLab software with default parameters.

B. Expression of a PL1 Nucleic Acid Molecule

A nucleic acid molecule comprising nucleotides 59 through 827 of SEQ ID NO:1, encoding a predicted mature flea PL1, was PCR amplified from the pBluescript™ clone described above as the template, using sense primer PL1-FE, having nucleotide sequence 5′ CGG GAT CCT GCT GAC AGG AAT TCG CCC AC 3′, having a BamHI site indicated in bold, designated herein as SEQ ID NO:50, and anti-sense primer PL1-RE, having nucleotide sequence 5′ CAT GGT ACC CCT GGT TTA AGC CTT ACT TAG C 3′, having a KpnI site indicated in bold, designated herein as SEQ ID NO:51. PCR reactions were performed using standard PCR reaction and thermocycling conditions described herein. The PCR product was digested with BamHI and KpnI and ligated into the vector pTrcHisB, available from Invitrogen, that had been digested with BamHI and KpnI and treated with alkaline phosphatase. The resulting recombinant molecule, referred to herein as pTrc-nCfPL1₇₆₉, was transformed into E. coli strain BL21, available from Novagen, to form recombinant cell E. coli:pTrc-nCfPL1₇₆₉. The recombinant cell was grown under standard conditions and then incubated in the presence of 0.5 μM IPTG to induce expression of recombinant protein, predicted to be a protein of approximately 32 kDa. Expression of protein was confirmed using Coomassie-blue-stained Tris-glycine gel and by Western blot using a T7 tag antibody which showed expression of an 32-kDa protein. The protein product was purified by liquid chromatography using a HiTrap™ chelating column charged with NiCl₂, available from Pharmacia, and was shown to contain the His tag of the vector when subjected to automated protein sequencing by Edman degradation.

C. Northern Blot Analysis

A Northern Blot analysis was conducted as follows to determine whether PL1 is expressed exclusively in HMT tissues. HMT tissues were dissected from 1000 adult cat blood-fed C. felis having a male to female ratio of 1:4. Total RNA was separately extracted from HMT tissues and the HMT-less carcasses that resulted from these dissections as follows. The tissues were frozen at −80° C., ground into a powder with a mortar and pestle, and the powders were equally divided into four 2-ml eppendorf tubes each containing 1 ml of lysis buffer. The lysis buffer contained 4 M guanidinium thiocyanate, 25 mM sodium citrate, pH 7.0, 3% sarcosyl, 0.5M 2-mercaptoethanol, 0.1% antifoam, and 1 mM aurintricarboxylic acid, all available from Sigma Chemical Corporation, St. Louis, Mo. After mixing, the tubes were spun at 14,000 rpm for 2 minutes and the supernatants were transferred to separate 2 ml eppendorf tubes containing 250 μl of phenol, available from Aldrich, Milwaukee, Wis. After mixing, the tubes were spun at 14,000 rpm for 5 minutes and the supernatants were transferred to new 2-ml tubes. This process was repeated 3 times until no proteinaceous matter was visible at the phenol/lysis buffer interface, then 250 μl of chloroform was added to each tube and the contents mixed and spun at 14,000 rpm for 5 minutes followed by transferring the supernatant to a new tube. A volume of isopropanol equal to the volume of the supernatant was added to each tube and the tubes placed on ice for 5 minutes. The tubes were then spun at 14,000 rpm at room temperature for 15 minutes, the supernatants were removed and discarded and the remaining RNA pellets were washed with 70% ethanol and dried. The RNA pellets were resuspended in 100 μl of TE (10 mM Tris, 1 mM ethylenediaminetetraacetic acid (EDTA)). The quantity of RNA in each tube was then determined using a spectrophotometer.

Approximately 10 μg of each RNA was added to separate tubes containing 18.75 μl of loading buffer, which consists of 50% formamide, 16% formaldehyde, 17% water, 7% glycerol, 1×MOPS buffer (a 1:20 dilution of 0.4 M 93-[N-morpholino]propanesulfonic acid (MOPS), 0.1 M sodium acetate, and 20 mM EDTA), 10 μl ethidium bromide, and 10 μl bromophenol blue dye, all available from Sigma. The tubes were heated to 95° C. for 2 minutes then placed on ice. The RNA samples were separated by gel electrophoresis on a 1.5% agarose gel with 3.2% formaldehyde and 1×MOPS buffer; the gel was then soaked in water for 30 minutes prior to transfer to remove excess formaldehyde. The gel was then transferred using standard techniques, described by Sambrook et al., ibid, with 10×SSPE as the transfer buffer onto Nytran® nylon membrane, available from Schleicher and Schuell Inc., Keene, N.H. The membrane was UV cross-linked using the Stratalinker®, available from Stratagene, then prehybridized at 42° C. in 50% formamide, 5×SSPE, 1.2% SDS, 5×Denhardt's reagent, 2.5 mM EDTA, and 100 μg/ml salmon sperm DNA. A probe comprising the PL1 nucleic acid molecule, nCfPL1₁₀₉₆ was labeled with α-³²P-ATP using a DNA labeling kit, available from Amersham and added to the buffer at a concentration of approximately 1×10⁶ cpm/ml, and allowed to hybridize for 18 hours at 42° C. The blot was then washed as follows: 10 minutes at 42° C. in 4×SSPE and 1% SDS; 10 minutes at 42° C. in 2×SSPE and 1% SDS; 10 minutes 42° C. with 0.5×SSPE and 0.5×SDS; and 10 minutes at 42° C. with 0.25×SSPE and 0.25% SDS. The blot was then exposed to film for 1 hour, and the film was developed using standard procedures. Analysis of the developed film revealed that PL1 mRNA was present in HMT tissues but was not present in non-HMT tissues.

Northern Blot analysis was also conducted to determine whether PL1 mRNA is expressed only in certain stages of the flea life cycle and whether PL1 mRNA expression is influenced by feeding. Total RNA was extracted as described above from 1000 fleas at each of the following flea life stages; eggs; first instar larvae; third instar larvae; wandering larvae and pupae as well as from 1000 adult fleas under the following feeding conditions: unfed; fed on cat blood for 15 minutes; fed on cat blood for 2 hours; fed on cat blood for 8 hours; and fed on cat blood for 24 hours. Each RNA sample was separated by gel electrophoresis, transferred to nylon membrane and hybridized with α-³²P-ATP labeled nCfPL1₄₂₉ probe as described above. Analysis of the developed film revealed that PL1 mRNA was detected in all adult fleas tested regardless of feeding conditions but was not detected in any of the non-adult life stages.

EXAMPLE 4

This Example describes the further isolation and characterization of a Peritrophin-like cDNA nucleic acid molecules, referred to herein as PL2.

A cDNA designated clone 2232-23 was isolated from the unsubtracted HMT library as described in Example 1, denoted herein as SEQ ID NO:6. Analysis of clone 2232-23 indicated that the cDNA, denoted nCfPL2₄₄₅ is 445 nucleotides in length. Translation of the coding strand of nCfPL2₄₄₅ suggests that nucleic acid molecule nCfPL2₄₄₅ encodes a partial-length Peritrophin-like protein of 113 amino acids, referred to herein as PCfPL2₁₁₃, assuming a stop coding spanning nucleotides 342 through 344 of nCfPL2₄₄₅.

Additional coding sequence corresponding to the 5′ end of nCfPL2₄₄₅ was isolated by PCR performed using a RACE cDNA pool prepared as described in Example 2 as template. A first PCR reaction was performed using reverse primer PL2-R1, which is complementary to nucleotides 167 through 187 of the nCfPL2₄₄₅ cDNA, having a nucleic acid sequence 5′ GTC TGG AAG CTC AGG AAG AGG 3′, denoted herein as SEQ ID NO:52, in conjunction with forward Adapter Primer 1, having a nucleic acid sequence 5′ CCA TCC TAA TAC GAC TCA CTA TAG GGC 3′, denoted herein as SEQ ID NO:53, under the following thermocycling conditions: (1) 94° C. for 30 seconds, (2) 5 cycles of 94° C. for 10 seconds and 72° C. for 4 minutes, (3) 5 cycles of 94° C. for 10 seconds and 70° C. for 4 minutes, and (4) 25 cycles of 94° C. for 10 seconds then 68° C. for 4 minutes. The product of this reaction was diluted 1:50 and used as template for a second PCR reaction as follows. Forward adapter primer 2, having nucleic acid sequence 5′ ACT CAC TAT AGG GCT CGA GCG GC 3′, denoted herein as SEQ ID NO:54, was used with reverse primer PL2-R2, which is complementary to nucleotides 29-52 of the nCfPL2₄₄₅ cDNA, having a nucleic acid sequence 5′ GTA ATA TGC GTG ACA ATC GTG TGG 3′, denoted herein as SEQ ID NO:55, using the thermocycling conditions described for the first PCR reaction. The resulting product was gel purified to reveal a distinct band corresponding to a nucleic acid molecule of approximately 900 bp in length. The fragment was then ligated into the pCR II TA Cloning vector, available from Qiagen, and sequenced using an ABI PRISM 377 automatic DNA Sequencer. Sequencing revealed that nucleotides 791-835 of the fragment had 100% identity with nucleotides 1-45 of the nCfPL2₄₄₅ cDNA. The 900 nucleotide and 445 nucleotide sequences were aligned to form a contiguous sequence, denoted nCfPL2₁₂₇₉, which is 1279 nucleotides in length, having a coding strand with nucleic acid sequence SEQ ID NO:7 and a complementary sequence having SEQ ID NO:8. Translation of SEQ ID NO:7 suggests that nucleic acid molecule nCfPL2₁₂₇₉ encodes a non full-length Peritrophin-like protein of 391 amino acids.

In order to isolate additional sequence 5′ to SEQ ID NO:7, nested PCR reactions were performed using the RACE cDNA pool as template. For the first PCR, forward adapter primer AP1 (SEQ ID NO:53) was used with reverse primer PL2-R1 (SEQ ID NO:52) under standard PCR reaction conditions and the following thermocycling conditions: (1) 94° C. for 1 minute, (2) 5 cycles of 94° C. for 20 seconds and 70° C. for 1 minute, (3) 5 cycles of 94° C. for 20 seconds and 68° C. for 1 minute, (4) 10 cycles of 94° C. for 20 seconds and 66° C. for 1 minute. The products of this reaction were diluted 1:50 in water and used as template for the second, nested PCR. The second PCR reaction used forward adapter primer AP2 in conjunction with reverse primer PL2-R5, which is complementary to nucleotides 70-93 of SEQ ID NO:7, having a nucleotide sequence 5′ CGG TGC AAG TTA TAG AAC CTT CCG 3′, denoted herein as SEQ ID NO:56 under standard PCR reaction conditions using the following thermocycling conditions: (1) 94° C. for 1 minute, (2) 5 cycles of 94° C. for 20 seconds and 70° C. for 1 minute, (3) 5 cycles of 94° C. for 20 seconds and 68° C. for 1 minute, (4) 40 cycles of 94° C. for 20 seconds and 66° C. for 1 minute. The products of this reaction were separated by agarose gel electrophoresis and a band approximately 279 nucleotides in length was excised from the gel and purified. The fragment, referred to as nCfPL2₂₇₉, having a coding nucleic acid sequence designated SEQ ID NO:9 and a complementary sequence designated SEQ ID NO:10, was then ligated into the pCROII TA Cloning vector, available from Qiagen, and sequenced as described above. Sequencing revealed that nucleotides 228-279 of nCfPL2₂₇₉ were identical to nucleotides 42-93 of SEQ ID NO:7, however, nucleotides 186-228 of nCfPL2₂₇₉ had no significant similarity to SEQ ID NO:7. This discrepancy may be the result of alternative RNA splicing or may be an artifact of the cDNA pool. To determine the reason for this discrepancy, additional fragments corresponding to this region were isolated by PCR from flea cDNA libraries from adult midguts, hindgut and Malpighian tubules and mixed instar larvae using techniques described herein. Sequence analysis of fragments obtained from these libraries revealed that these fragments were identical in sequence to the sequence of nCfPL2₂₇₉, therefore, the region of SEQ ID NO:7 which did not align to nCfPL2₂₇₉ was deemed to be an artifact and was not used in subsequent alignments.

The PL2 sequences described above were aligned to form a contiguous sequence, denoted nCfPL2₁₄₆₅, which is 1465 nucleotides in length, having a coding strand with nucleic acid sequence SEQ ID NO:11 and a complementary sequence having SEQ ID NO:13. Translation of SEQ ID NO:11 suggests that nucleic acid molecule nCfPL2₁₄₆₅ encodes a full-length Peritrophin-like protein of 453 amino acids, referred to herein as PCfPL2₄₅₃, having an amino acid sequence represented by SEQ ID NO:12, assuming an initiation codon spanning from nucleotide 3 through nucleotide 5 of SEQ ID NO:11 and a termination codon spanning from nucleotide 1362 through nucleotide 1364 of SEQ ID NO:11. The coding region encoding PCfPL2₄₅₃, is represented by nucleic acid molecule nCfPL2₁₃₅₉, having a coding strand with the nucleic acid sequence represented by SEQ ID NO:14 and a complementary strand with nucleic acid sequence represented by SEQ ID NO:15. The amino acid sequence of SEQ ID NO:12, predicts that PCfPL2₄₅₃ has an estimated molecular weight of 49 kDa and an estimated isoelectric point (pI) of 4.7.

Comparison of amino acid sequence SEQ ID NO:12 with amino acid sequences reported in GenBank indicates that SEQ ID NO:12 showed the most homology, i.e., 28% identity, with a Drosophila melanogaster locus AE003474 protein (Accession # AAF47629). Comparison of SEQ ID NO:14 with nucleic acid sequences reported in GenBank indicates that SEQ ID NO:14 showed the most homology, i.e., 50% identity, with a Penaeus semisulcatus (a crustacean) peritrophin-like protein 1 cDNA (Accession # AF095580). Percent identity calculations were performed using the SeqLab software with default parameters.

EXAMPLE 5

This Example describes the further characterization and expression of a flea Peritrophin-like sequence cDNA, referred to herein as PL3.

A. Isolation of PL3 Nucleic Acid Molecules

A cDNA designated clone 2240-17 was isolated from the unsubtracted HMT library as described in Example 1. Analysis of clone 2240-17 indicated that the cDNA, denoted nCfPL3₃₈₇, is 387 nucleotides in length, having a coding strand with nucleic acid sequence SEQ ID NO:16 and a complementary sequence having SEQ ID NO:18. Translation of SEQ ID NO:16 suggests that nucleic acid molecule nCfPL3₃₈₇ encodes a full-length Peritrophin-like protein of 81 amino acids, referred to herein as PCfPL3₈₁, having an amino acid sequence represented by SEQ ID NO:17, assuming the initiation codon spans from nucleotide 20 through nucleotide 22 of SEQ ID NO:16 and the termination codon spans from nucleotide 263 through nucleotide 265 of SEQ ID NO:16. The coding region encoding PCfPL3₈₁, is represented by nucleic acid molecule nCfPL3₂₄₃, having a coding strand with the nucleic acid sequence represented by SEQ ID NO:19 and a complementary strand with nucleic acid sequence represented by SEQ ID NO:20. The amino acid sequence of SEQ ID NO:17, predicts that PCfPL3₈₁ has an estimated molecular weight of 9.1 kDa and an estimated isoelectric point (pI) of 3.64.

Comparison of amino acid sequence SEQ ID NO:17 with amino acid sequences reported in GenBank indicates that SEQ ID NO:17 showed the most homology, i.e., 34.2% identity, with a Anopheles gambiae peritrophin 1 protein (Accession # AAC39127). Comparison of SEQ ID NO:19 with nucleic acid sequences reported in GenBank indicates that SEQ ID NO:19 showed the most homology, i.e., 37% identity, with a Anopheles gambiae chloride intracellular channel 2 (Accession # AF030431). Percent identity calculations were performed using the SeqLab software with default parameters.

B. Expression of a PL3 Protein

In order to express a PL3 protein, the entire coding region was amplified by PCR and then ligated into the E. coli expression vector pTrcHisB, available from Invitrogen, as follows. Forward primer PL3FE, which corresponds to nucleotides 70-93 of SEQ ID NO:16, having the sequence 5′ CGG GAT CCC GAA TAT GCT GAC GTA GAT GTG TG 3′, denoted SEQ ID NO:57, and having a BamHI restriction endonuclease site indicated in bold, was used in conjunction with reverse primer PL3RE, which is complementary to nucleotides 245-269 of SEQ ID NO:16, having the sequence 5′ GGA ATT CTG TTT TAT TCT GGT TGG TAA CAT TC 3′, denoted herein as SEQ ID NO:58 and having an EcoRI restriction endonuclease site indicated in bold, in a PCR reaction using SEQ ID NO:16 as the template under standard PCR reaction conditions and the following thermocycling conditions: (1) 94° C. for 30 seconds, (2) 25 cycles of 94° C. for 10 seconds, 55° C. for 10 seconds and 72° C. for 3 minutes. The reaction product was separated on a 1.5% agarose gel, and a band corresponding to an approximately 200 nucleotide molecule, as visualized by agarose gel electrophoresis and ethidium bromide staining, was cut from the gel and purified using a QIAquick Gel Extraction Kit, available from Qiagen.

The product of the PCR reaction was the digested with BamHI and EcoRI restriction endonucleases, available from New England BioLabs, Inc. for 18 hours at 37° C., purified using the QIAquick Nucleotide Removal Kit, available from Qiagen, and ligated into the vector pTrcHisB which had been similarly digested, treated with shrimp alkaline phosphatase, available from New England BioLabs, Inc., for 30 minutes at 37° C., and purified. Following standard transformation procedures into E. coli BL-21 competent cells, a bacterial clone containing the plasmid pTrcPL3₂₀₀ was isolated. DNA sequence analysis of the clone confirmed that nucleotides 70 through 269 of SEQ ID NO:16 had been successfully ligated into the pTrcHisB expression vector in frame with the N-terminal T7 Tag epitope encoded by the vector. The recombinant protein encoded thereby is predicted to be 97 amino acids in length (including the T7 Tag) and have a molecular mass of 10.9 kDa, including the T7 Tag, and have a pI of 4.08.

The 97 amino acid recombinant PL3 protein described above was expressed as follows. Five mls of Luria broth were innoculated with a glycerol stock of E. coli BL-21 competent cells, available from Novagen, Madison, Wis., that had been transformed with the pTrcPL3₂₀₀ plasmid prepared as described above and allowed to grow overnight at 37° C. under selection with 100 μg/ml ampicillin. A 1-ml aliquot of this culture was then used to inoculate 10 mls of fresh Luria broth containing 100 μg/ml ampicillin and the culture was allowed to grow to an approximate OD reading of 0.5. A 1 ml aliquot of the culture was removed, the cells were pelleted by centrifugation and the supernatant discarded. The cells were resuspended in a solution of 100 μl PBS and 100 μl of 2×SDS-PAGE loading buffer (100 mM Tris pH 6.8, 4% SDS, 20% glycerol, 0.02% bromophenol blue, and 10% 2-mercaptoethanol). Following removal of the 1 ml aliquot described above, IPTG was added to the remaining 9 ml culture to a final concentration of 5 mM of IPTG, the culture was incubated at 37° C. for an additional 60 minutes, 1 ml was removed and the OD measured at approximately 0.6. The cells in this 1 ml sample were then pelleted by centrifugation and resuspended in a solution of 120 μl of PBS and 120 μl of SDS-PAGE loading buffer. Equal volumes of the IPTG-induced and uninduced lysates were loaded onto a 14% Tris-Glycine SDS-PAGE gel, available from Novex, San Diego, Calif. Following electrophoresis, the proteins were transferred from the SDS-PAGE gel to a nitrocellulose membrane and a Western blot analysis was performed using the T7 tag antibody, available from Novagen, which revealed an approximately 18 kDa protein was induced by IPTG. The fact that the recombinant nCfPL3₂₀₀ protein ran at a higher molecular weight than predicted is consistent with previous published results for other peritrophin proteins, and is thought to be due in part to the characteristically low pI of these proteins; See Tellam et al., 1999, Insect Biochemistry and Molecular Biology, 29:87-101. Sequence analysis of this protein indicates that it contained the N-terminal T7 Tag encoded by the vector.

Four flasks, each containing 1 liter of Luria broth with 100 μg/ml ampicillin were inoculated with a starter culture of 5 ml of E. coli BL-21 cells transformed with the pTrcnCfPL3₂₀₀ plasmid as described above. The cultures were allowed to grow at 37° C. until the optical density reached approximately 0.500, at which time a 1 ml aliquot was removed from each flask as the pre-induction sample. IPTG was added to each 1 liter flask to a final concentration of 0.5 mM and the cultures allowed to grow at 37° C. for 135 additional minutes, at which time a 1 ml aliquot was removed from each flask as the post-induction sample. The 1 ml aliquots were centrifuged, the supernatants were discarded and the pellets were resuspended in 100 μl 2×SDS-PAGE loading buffer per each 0.5 optical density units measured. The pre-induction and post induction samples were then tested for recombinant PL3 protein expression using standard Western blot techniques and the T7 Tag antibody. A protein running at approximately 18 kDa was detected in the post-induced but not in the pre-induced samples.

The cells from the remaining 4 liters of culture were centrifuged, the supernatants were discarded and the cell pellets were combined and resuspended in 120 mls of buffer A (50 mM Tris, PH 8.0, 20 mM NaCl, 1 mM phenylmethylsulfonyl fluoride (PMSF)). The sample was then passed through a microfluidizer five times then rocked at 4° C. for 20 minutes. The sample was then centrifuged for 30 minutes and the supernatant collected. Western blot analysis of the supernatant showed that the recombinant nCfPL3₂₀₀ protein was soluble in the first buffer A extraction. The buffer A supernatant containing the recombinant nCfPL3₂₀₀ protein was then further purified by a nickel column, a Q2 anion exchange chromatography column, and cation exchange chromatography, using techniques well known to those of skill in the art.

EXAMPLE 6

This Example describes the characterization, expression, and Northern Blot analysis of a Peritrophin-like sequence cDNA, referred to herein as PL4.

A. Isolation of PL4 Nucleic Acid Molecules

A cDNA designated clone 2244-71 was isolated from the unsubtracted HMT library as described in Example 1. Analysis of clone 2244-71 indicated that the cDNA, denoted nCfPL4₉₆₀, is 960 nucleotides in length, having a coding strand with nucleic acid sequence SEQ ID NO:21 and a complementary sequence having SEQ ID NO:22. Translation of SEQ ID NO:21 suggests that nucleic acid molecule nCfPL4₉₆₀ encodes a partial-length Peritrophin-like protein of 285 amino acids. Additional sequence 5′ to nCfPL4₉₆₀ was isolated by PCR using the RACE cDNA pool described in Example 2 as the template, as follows. Adapter Primer 1, i.e. SEQ ID NO:53, was used as the forward primer in conjunction with reverse primer PL4-R1, which is complementary to nucleotides 229-251 of SEQ ID NO:21, having a nucleic acid sequence 5′ GAT ATC CAC TTT GAT CAG CGC AC 3′, denoted herein as SEQ ID NO:59 in a PCR reaction under standard PCR reaction conditions and the following thermocycling conditions: (1) 94° C. for 30 seconds, (2) 5 cycles of 94° C. for 10 seconds and 72° C. for 4 minutes, (3) 5 cycles of 94° C. for 10 seconds and 70° C. for 4 minutes, (4) 25 cycles of 94° C. for 10 seconds then 68° C. for 4 minutes. The products of this reaction were diluted 1:50 and used as template in a second PCR reaction using Adapter Primer 2, i.e. SEQ ID NO:54, as the forward primer and reverse primer PL4-R2, which is complementary to nucleotides 58-78 of SEQ ID NO:21, having a nucleic acid sequence 5′ GGT ACT ACT CCT GGT GCG GGC 3′, denoted herein as SEQ ID NO:60, using the thermocycling conditions described for the first PCR reaction. The products of this reaction were gel purified as previously described and the fragment was ligated into the pCR II TA Cloning vector, available from Qiagen, and sequenced to reveal a fragment of approximately 150 nucleotides in length. Sequence analysis revealed that nucleotides 68-146 of the fragment had 100% identity with nucleotides 1-79 of nCfPL4₉₆₀. The two sequences were aligned to form a contiguous sequence of 1029 nucleotides in length, referred to as nCfPL4₁₀₂₉, having a coding strand with SEQ ID NO:23 and a complementary strand having SEQ ID NO:24. However, the contiguous sequence did not appear to encode a starting methionine in the predicted protein sequence; thus, a second attempt to isolate the remaining coding sequences at the 5′ end was performed as follows. A first PCR reaction was performed with Adapter Primer 1 (SEQ ID NO:53) as the forward primer and PL4-R2 (SEQ ID NO:60) as the reverse primer using the RACE cDNA pool as the template under the thermocycling conditions described above. The products of this reaction were diluted 1:50 and used as the template in a second PCR reaction which used Adapter Primer 2 (SEQ ID NO:54) as the forward primer and reverse primer PL4-R4, which is complementary to nucleotides 58-80 of SEQ ID NO:23, having the nucleic acid sequence 5′ CCG TCG ACA TTA AAC TCA CCA TC 3′, denoted SEQ ID NO:61, under the thermocycling conditions described for the first PCR reaction. The products of this reaction were gel purified as previously described and the fragment was ligated into the pCR II TA Cloning vector, available from Qiagen, and sequenced to reveal a fragment of approximately 100 nucleotides in length. Sequence analysis revealed that nucleotides 21-101 of the fragment had 100% identity with nucleotides 1-81 of SEQ ID NO:21. The two sequences were aligned to form a contiguous sequence that is 1048 nucleotides in length, referred to herein as nCfPL4₁₀₄₈, having a coding strand with SEQ ID NO:25 and a complementary strand with SEQ ID NO:27. Translation of SEQ ID NO:25 suggests that nucleic acid molecule nCfPL4₁₀₄₈ encodes a full-length Peritrophin-like protein of 285 amino acids, referred to herein as PCfPL4₂₈₅, having an amino acid sequence represented by SEQ ID NO:26, assuming the initiation codon spans from nucleotide 19 through nucleotide 21 of SEQ ID NO:25 and the termination codon spans from nucleotide 874 through nucleotide 876 of SEQ ID NO:25. The coding region encoding PCfPL4₂₈₅ is represented by nucleic acid molecule nCfPL4₈₅₅, having a coding strand with the nucleic acid sequence represented by SEQ ID NO:28 and a complementary strand with nucleic acid sequence represented by SEQ ID NO:29. The amino acid sequence of SEQ ID NO:26 predicts that PCfPL4₂₈₅ has an estimated molecular weight of 31.4 kDa and an estimated isoelectric point (pI) of 6.99.

Comparison of amino acid sequence SEQ ID NO:26 with amino acid sequences reported in GenBank indicates that SEQ ID NO:26 showed the most homology, i.e., 31.5% identity, with a Drosophila melanogaster Gasp precourser (Accession # AAD09748). Comparison of SEQ ID NO:28 with nucleic acid sequences reported in GenBank indicates that SEQ ID NO:28 showed the most homology, i.e., 39.4% identity, with a Drosophila melanogaster Gasp precourser (Accession #AF070734). Percent identity calculations were performed using the SeqLab software with default parameters.

B. Northern Blot Analysis

A Northern Blot analysis was conducted as described in Example 3 to determine whether PL4 mRNA is expressed only in certain life stages of the flea life cycle and whether PL4 mRNA is expressed only in HMT tissue. Total RNA was extracted from eggs, first instar larvae, third instar larvae, and wandering larvae, pupae, unfed adults, and adults fed on cat blood for 0.25, 2, 8, or 24 hours, respectively. In addition, total RNA was extracted from hindguts and Malpighian tubules extracted from 24-hour cat blood-fed adult fleas, and from the remaining body parts following the removal of hindguts and Malpighian tubules. Each RNA sample was separated by gel electrophoresis, transferred to nylon membranes and hybridized with α-³²P-ATP labeled nCfPL4₉₆₀ using the conditions described in Example 3.

The results of the Northern blot assay were complex. Although stringent conditions were used, several bands with distinct expression patterns were seen. An approximately 1600 nucleotide message was detected in the egg, first instar, third instar and wandering larval stages only. An approximately 1500 nucleotide message was detected in all lifestages and adult fed timepoints, but with the strongest signals in the egg, first instar larval, and unfed adult stages. A third message, of approximately 1200 nucleotides, was detected in the egg, first instar larval, pupal, and adult lifestages, including all unfed and fed adult timepoints. All three of the messages detected were seen only in the HMT tissues, and were not detected in the carcass tissues.

The detection of three mRNAs instead of one may be the result of the expression of three highly homologous transcripts. It has been reported in the literature that peritrophin gene families have been found that consist of a number of highly related genes, See Schorderet et al., 1998, Insect Biochemistry and Molecular Biology 28, 99-111. It is possible that these transcripts represent the products of such a family or that the messages are the RNA products of alternative splicing from a single gene locus.

The coding region of SEQ ID NO:25, was PCR amplified from the RACE cDNA pool described above as the template, using sense primer PL4FE, having nucleotide sequence 5′ CGG GAT CCT TAT GAT GGT GAG TTT AAT GTC G 3′, which corresponds to nucleotides 75-96 of SEQ ID NO:25, having a BamHI site indicated in bold, designated herein as SEQ ID NO:62, and anti-sense primer PL4RE, having nucleotide sequence 5′ GGG GTA CCT TAA TAT AAT TTA GGT TTC CTC TCG C 3′, which is complementary to nucleotides 851-876 of SEQ ID NO:25, having a KpnI site indicated in bold, designated herein as SEQ ID NO:63. PCR reactions were performed using the following amplification cycles: (a) one cycle at 94° C. for thirty seconds; (b) thirty cycles at 94° C. for twenty seconds, 68° C. for thirty seconds, and 72° C. for three minutes; and (c) one cycle at 72° C. for five minutes, in reactions containing 2.5 mM MgCl₂, 0.2 mM dNTPs, 1 μM of each primer, 0.5 μl of 5 U/μl Taq polymerase, 1 μl of 1 μg/μl template, and 3 μl of 10×Taq buffer. The products of this reaction were separated on a 1.5% agarose gel, and the appropriate band cut from the gel and purified using the QIAquick Gel Extraction Kit, available from Qiagen, Valencia, Calif. The resulting nucleic acid molecule, referred to herein as nCfPL4₈₀₂, is approximately 802 nucleotides in length, having a coding strand designated SEQ ID NO:30 and a complementary strand designated SEQ ID NO:32. Translation of SEQ ID NO:30 indicates that SEQ ID NO:30 encodes an approximately 266 amino acid protein, designated SEQ ID NO:31, assuming a first codon at nucleotides 2 through 4 and a stop codon at nucleotides 800 through 802 of SEQ ID NO:30.

The purified PCR product was digested with BamHI and KpnI, purified using a QIAquick Nucleotide Removal Kit, available from Qiagen, and ligated into the vector pTrcHisB, available from Invitrogen, that had been similarly purified, digested with BamHI and KpnI and treated with alkaline phosphatase. DNA sequence analysis of the clone confirmed that nucleotides 75 through 876 of SEQ ID NO:25 had been successfully ligated into the pTrcHisB expression vector in frame with the N-terminal T7 Tag epitope encoded by the vector. The resulting recombinant molecule, referred to herein as pTrc-nCfPL4₈₉₄ (which includes the T7 tag), was transformed into E. coli strain BL21, available from Novagen Inc., Madison, Wis., to form recombinant cell E. coli:pTrc-nCfPL4₈₉₄. Recombinant molecule pTrc-nCfPL4₈₉₄ is predicted to encode a protein including the T7 Tag of 298 amino acids in length, having a molecular mass of 32.8 kDa, and a pI of 5.97.

Recombinant cell E. coli:pTrc-nCfPL4₈₉₄ was grown as described in Example 5 and then incubated in the presence of 0.5 mM isopropylthio-β-galactoside (IPTG) to induce expression of recombinant protein PCfPL4₂₉₈. Expression was confirmed by Western blot using a T7 tag antibody, available from Novagen, which showed expression of an 48-kDa protein. Sequence analysis of PCfPL4₂₉₈ indicates that it contained the N-terminal T7 Tag encoded by the vector. The fact that PCfPL4₂₉₈ ran at a higher molecular weight than predicted is consistent with previous published results for other peritrophin proteins, and is thought to be due in part to the characteristically low pI of these proteins, See Tellam et al. ibid.

Recombinant protein PCfPL4₂₉₈ was produced and purified as follows. Four flasks containing 1 liter each of Luria broth with 100 ug/ml ampicillin were innoculated with E. coli BL21 cells transformed with the pTrc PCfPL4₂₉₈ plasmid as described above. The cultures were allowed to grow at 37° until the optical density (OD₆₀₀) reached approximately 0.500 then a one ml aliquot was removed as the pre-induction sample. Next, IPTG was added to a final concentration of 0.5 mM and the cultures allowed to grow at 37° C. for 135 minutes. A one-ml aliquot was then removed as the post-induction sample. Both one-ml aliquots were centrifuged to pellet the cells. The cells were resuspended in 100 μl 2×SDS-PAGE loading buffer for each 0.5 optical density unit measured. The pre-induction and post induction samples were then tested for expression of PCfPL4₂₉₈ using by Western blot with the T7 Tag antibody described previously. A protein running at approximately 48 kDa was detected in the post-induced but not in the pre-induced samples.

The cells from the remaining 4 liters of culture were pelleted by centrifugation and resuspended in 120 ml of Buffer A. The sample was passed through a microfluidizer five times, rocked at 4° for 20 minutes, then centrifuged for 30 minutes and the supernatant collected. Western blot analysis of the supernatant showed that the recombinant protein PCfPL4₂₉₈ was soluble in Buffer A. Protein contained in the Buffer A supernatant was then purified by nickel ion exchange chromatography and hydroxyapatite chromatography.

EXAMPLE 7

This Example describes the characterization and expression of a flea Peritrophin-like sequence cDNA, referred to herein as PL5.

cDNA nucleic acid molecules 2109-28, 2234-75, 2241-70, 2164-22, 2183-05, and 2162-56, designated SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37 and SEQ ID NO:38, respectively, were isolated from the unsubtracted HMT library as described in Example 1. Sequence analysis revealed that these clones contain overlapping sequence to form a 1513-nucleotide cDNA consensus sequence denoted nCfPL5₁₅₁₃, having a coding strand designated SEQ ID NO:39 and a complementary strand designated SEQ ID NO:41. Translation of the coding strand of nCfPL5₁₅₁₃ suggests that nucleic acid molecule nCfPL5₁₅₁₃ encodes a partial-length Peritrophin-like protein of 339 amino acids, referred to herein as PCfPL5₃₃₉ with an amino acid sequence designated SEQ ID NO:40, assuming a stop codon at nucleotides 1018-1020 of nCfPL5₁₅₁₃. Protein PCfPL5₃₃₉ has a predicted weight of 36.5 kDa and a predicted pI of 6.98.

Additional coding sequence 5′ to nCfPL5₁₅₁₃ was isolated by PCR performed using a RACE cDNA pool prepared as described in Example 2 as template. A first PCR reaction was performed using reverse primer PL5-R1, which is complementary to nucleotides 513-533 of the nCfPL5₁₅₁₃ cDNA, having nucleic acid sequence 5′ GCG CAT GTA AAA CGA CCC ACG 3′, denoted herein as SEQ ID NO:64, in conjunction with the universal primer mix from the SMART RACE cDNA amplification kit described in Example 2, which contains two primers, the first having nucleic acid sequence 5′ CTA ATA CGA CTC ACT ATA GGG CAA GCA GTG GTA ACA ACG CAG AGT 3′, and the second having nucleotide sequence 5′ CTA ATA CGA CTC ACT ATA GGG C 3′, denoted herein as SEQ ID NO:65 and SEQ ID NO:66 respectively, under the following thermocycling conditions: (1) 94° C. for 30 seconds, (2) 35 cycles of 94° C. for 30 seconds, 68 C. for 30 seconds and 72° C. for 3 minutes, (3) 72° C. for 7 minutes. The resulting product was gel purified to reveal a distinct band corresponding to nucleic acid molecule of approximately 850 nucleotides in length, referred to herein as nCfPL5₈₅₀. Fragment nCfPL5₈₅₀ was purified using a QLAquick Gel Extraction Kit, ligated into the pCR II TA Cloning vector, available from Invitrogen, and sequenced using an ABI PRISM 377 automatic DNA Sequencer, available from Perkin Elmer. Sequencing revealed that nucleotides 320-852 of nCfPL5₈₅₀ had 100% identity with nucleotides 1-533 of the nCfPL5₁₅₁₃ cDNA. The two sequences were aligned to form a contiguous sequence, denoted nCfPL5₁₈₃₂, which is 1832 nucleotides in length, having a coding strand with nucleic acid sequence SEQ ID NO:42 and a complementary sequence having SEQ ID NO:44.

Translation of SEQ ID NO:42 suggests that nucleic acid molecule nCfPL5₁₈₃₂ encodes a full-length Peritrophin-like protein of 397 amino acids, referred to herein as PCfCfPL5₃₉₇, having an amino acid sequence represented by SEQ ID NO:43, assuming a start codon spanning nucleotides 146 through 148 and a stop codon spanning nucleotides 1337 through 1339 of SEQ ID NO:42. The coding region encoding PCfCfPL5₃₉₇, is represented by nucleic acid molecule nCfPL5₁₁₉₁, having a coding strand with the nucleic acid sequence represented by SEQ ID NO:45 and a complementary strand with nucleic acid sequence represented by SEQ ID NO:46. The amino acid sequence of PCfCfPL5₃₉₇ predicts that PCfCfPL5₃₉₇ has an estimated molecular weight of 43.2 kDa and an estimated pI of 7.4.

Comparison of amino acid sequence SEQ ID NO:43 with amino acid sequences reported in GenBank indicates that SEQ ID NO:43 showed the most homology, i.e., 28% identity with the protein encoded by a Drosophila melanogaster cDNA Accession # CAA19845.2. Comparison of SEQ ID NO:45 with nucleic acid sequences reported in GenBank indicates that SEQ ID NO:45 showed the most homology, i.e., 40% with a Trichoplusia ni insect intestinal mucin IIM22, GenBank Accession #AF000606. Percent identity calculations were performed using the SeqLab software with default parameters.

A nucleic acid molecule comprising nucleotides 194 through 1354 of SEQ ID NO:42, encoding a predicted mature PL5 protein, was PCR amplified from the HMT RACE pool cDNA described above as follows. Sense primer PL5-FE, having nucleotide sequence 5′ CGG GTA CCT TGG AGT CTC AAG AAC TAA TTC 3′, which corresponds to nucleotides 194-215 of SEQ ID NO:42 and having a KpnI site indicated in bold, designated herein as SEQ ID NO:67, was used in conjunction with anti-sense primer PL5-RE, having nucleotide sequence 5′ AGG AAT TCC ATA TAA CAC ACT CAC TAG GTA CAT GTA G 3′, which is complementary to nucleotides 194-215 of SEQ ID NO:42 and having an EcoRI site indicated in bold, designated herein as SEQ ID NO:68 under the following thermocycling conditions: (1) one cycle of 94° for 30 seconds, (2) 30 cycles of 94° for 30 seconds, 68° for 30 seconds, and 72° for 3 minutes, (3) one cycle of 72° for 7 minutes. The products of this reaction were separated on a 1.5% agarose gel, and the appropriate band cut from the gel and purified using the QIAquick Gel Extraction Kit, available from Qiagen. The resulting nucleic acid molecule, referred to herein as nCfPL5₁₁₆₁, has a coding strand designated SEQ ID NO:47 and a complementary strand designated SEQ ID NO:49. Translation of SEQ ID NO:47 indicates that SEQ ID NO:47 encodes an approximately 381 amino acid protein, the amino acid sequence of which is designated SEQ ID NO:48, assuming a first codon at nucleotides 1 through 3 and a stop codon at nucleotides 1144 through 1146 of SEQ ID NO:47. The purified product was digested with KpnI and EcoRI and ligated into the vector pTrcHisB, available from Invitrogen, that had been digested with KpnI and EcoRI and treated with alkaline phosphatase. An individual clone was isolated and the DNA extracted and sequenced. DNA sequence analysis of the clone confirmed that nucleotides 194-1354 of SEQ ID NO:42, had been successfully ligated into the pTrcHisB expression vector in frame with the N-terminal T7 Tag epitope encoded by the vector. The recombinant protein, including the T7 Tag, is predicted to be 423 amino acids in length, have a molecular mass of 45.9 kDa and have a pI of 6.36.

While various embodiments of the present invention have been described in detail, it is apparent that modifications and adaptations of those embodiments will occur to those skilled in the art. It is to be expressly understood, however, that such modifications and adaptations are within the scope of the present invention, as set forth in the following claims:

68 1 1096 DNA Ctenocephalides felis CDS (6)..(821) 1 tcaca atg aag ttc tta gga gct tta ttg gtt gca gtg ttt gcc ttg ggt 50 Met Lys Phe Leu Gly Ala Leu Leu Val Ala Val Phe Ala Leu Gly 1 5 10 15 gct gtg gct gct gac agg aat tcg ccc aca tat gtc cgc ggt ttc cca 98 Ala Val Ala Ala Asp Arg Asn Ser Pro Thr Tyr Val Arg Gly Phe Pro 20 25 30 gtg gga aga tcc aga gca cga aca aca ttt ggc aat gaa gaa ata aag 146 Val Gly Arg Ser Arg Ala Arg Thr Thr Phe Gly Asn Glu Glu Ile Lys 35 40 45 tgt act aat aag cag ttg gga aca ttt tgt cac gat tgt tct act ttg 194 Cys Thr Asn Lys Gln Leu Gly Thr Phe Cys His Asp Cys Ser Thr Leu 50 55 60 aag ttg tgc gct gga caa gaa acc cca att aca aca atc aat tgc aga 242 Lys Leu Cys Ala Gly Gln Glu Thr Pro Ile Thr Thr Ile Asn Cys Arg 65 70 75 gac tca aat tcc gat gct cca ttt tgt gta gat gat atg tgc tca tca 290 Asp Ser Asn Ser Asp Ala Pro Phe Cys Val Asp Asp Met Cys Ser Ser 80 85 90 95 aaa cct ggg gaa aac tgt aag acg gca gaa act aca tgc gcc gtt gta 338 Lys Pro Gly Glu Asn Cys Lys Thr Ala Glu Thr Thr Cys Ala Val Val 100 105 110 gga tat cag cca gat ccg aaa gac tgc aca aga tac tta ttc tgc aaa 386 Gly Tyr Gln Pro Asp Pro Lys Asp Cys Thr Arg Tyr Leu Phe Cys Lys 115 120 125 gat ggt aaa ggt cag gtt ttc gaa tgc cca cct aac tat gta tat gat 434 Asp Gly Lys Gly Gln Val Phe Glu Cys Pro Pro Asn Tyr Val Tyr Asp 130 135 140 cat tct aaa aat atg tgt aaa aag aaa tcg tca gaa gct gat tgc acc 482 His Ser Lys Asn Met Cys Lys Lys Lys Ser Ser Glu Ala Asp Cys Thr 145 150 155 gtc atg aaa tgc aca aat ccc aat tct ttt ata acc tat gca ccg gac 530 Val Met Lys Cys Thr Asn Pro Asn Ser Phe Ile Thr Tyr Ala Pro Asp 160 165 170 175 cca tca att tat gct tgg tgc aat gac aaa ttg caa ccg atc gta ctg 578 Pro Ser Ile Tyr Ala Trp Cys Asn Asp Lys Leu Gln Pro Ile Val Leu 180 185 190 aaa tgt gaa gac gac gtc aac gaa tgg ttt gac cca aaa tct ttc tcg 626 Lys Cys Glu Asp Asp Val Asn Glu Trp Phe Asp Pro Lys Ser Phe Ser 195 200 205 tgc aga act gca tgc aaa agt gaa aac gtt ttt tcc gat cga aga gat 674 Cys Arg Thr Ala Cys Lys Ser Glu Asn Val Phe Ser Asp Arg Arg Asp 210 215 220 tgt aaa aaa tat tat caa tgt ttc ttg gtt aac aac aaa tgg caa ata 722 Cys Lys Lys Tyr Tyr Gln Cys Phe Leu Val Asn Asn Lys Trp Gln Ile 225 230 235 aaa cat tat gat tgt cca aat ggc ttg cac ttt gat aaa acg gag ttg 770 Lys His Tyr Asp Cys Pro Asn Gly Leu His Phe Asp Lys Thr Glu Leu 240 245 250 255 cga tgc ata ccc acg cca ccc ggc gaa gaa tgc aaa agt gag att gct 818 Arg Cys Ile Pro Thr Pro Pro Gly Glu Glu Cys Lys Ser Glu Ile Ala 260 265 270 aag taaggcttaa accaggaaaa caatcttgaa tagactaatt aggattcaaa 871 Lys ttatcataaa gtagtcaatt aatataataa atacacaaat gatctgtgca attaaatata 931 aaaaatatgt ttaaaaatta aaatgtataa aattgtattt tatgtaagga gcacaaacaa 991 aatgtcctta actatagtaa tttctgatta tttaaaatat ataaatatag aagctttatg 1051 aaattacatg tatcttttta ataaaaataa atcgtttggg ccgtt 1096 2 272 PRT Ctenocephalides felis 2 Met Lys Phe Leu Gly Ala Leu Leu Val Ala Val Phe Ala Leu Gly Ala 1 5 10 15 Val Ala Ala Asp Arg Asn Ser Pro Thr Tyr Val Arg Gly Phe Pro Val 20 25 30 Gly Arg Ser Arg Ala Arg Thr Thr Phe Gly Asn Glu Glu Ile Lys Cys 35 40 45 Thr Asn Lys Gln Leu Gly Thr Phe Cys His Asp Cys Ser Thr Leu Lys 50 55 60 Leu Cys Ala Gly Gln Glu Thr Pro Ile Thr Thr Ile Asn Cys Arg Asp 65 70 75 80 Ser Asn Ser Asp Ala Pro Phe Cys Val Asp Asp Met Cys Ser Ser Lys 85 90 95 Pro Gly Glu Asn Cys Lys Thr Ala Glu Thr Thr Cys Ala Val Val Gly 100 105 110 Tyr Gln Pro Asp Pro Lys Asp Cys Thr Arg Tyr Leu Phe Cys Lys Asp 115 120 125 Gly Lys Gly Gln Val Phe Glu Cys Pro Pro Asn Tyr Val Tyr Asp His 130 135 140 Ser Lys Asn Met Cys Lys Lys Lys Ser Ser Glu Ala Asp Cys Thr Val 145 150 155 160 Met Lys Cys Thr Asn Pro Asn Ser Phe Ile Thr Tyr Ala Pro Asp Pro 165 170 175 Ser Ile Tyr Ala Trp Cys Asn Asp Lys Leu Gln Pro Ile Val Leu Lys 180 185 190 Cys Glu Asp Asp Val Asn Glu Trp Phe Asp Pro Lys Ser Phe Ser Cys 195 200 205 Arg Thr Ala Cys Lys Ser Glu Asn Val Phe Ser Asp Arg Arg Asp Cys 210 215 220 Lys Lys Tyr Tyr Gln Cys Phe Leu Val Asn Asn Lys Trp Gln Ile Lys 225 230 235 240 His Tyr Asp Cys Pro Asn Gly Leu His Phe Asp Lys Thr Glu Leu Arg 245 250 255 Cys Ile Pro Thr Pro Pro Gly Glu Glu Cys Lys Ser Glu Ile Ala Lys 260 265 270 3 1096 DNA Ctenocephalides felis 3 aacggcccaa acgatttatt tttattaaaa agatacatgt aatttcataa agcttctata 60 tttatatatt ttaaataatc agaaattact atagttaagg acattttgtt tgtgctcctt 120 acataaaata caattttata cattttaatt tttaaacata ttttttatat ttaattgcac 180 agatcatttg tgtatttatt atattaattg actactttat gataatttga atcctaatta 240 gtctattcaa gattgttttc ctggtttaag ccttacttag caatctcact tttgcattct 300 tcgccgggtg gcgtgggtat gcatcgcaac tccgttttat caaagtgcaa gccatttgga 360 caatcataat gttttatttg ccatttgttg ttaaccaaga aacattgata atatttttta 420 caatctcttc gatcggaaaa aacgttttca cttttgcatg cagttctgca cgagaaagat 480 tttgggtcaa accattcgtt gacgtcgtct tcacatttca gtacgatcgg ttgcaatttg 540 tcattgcacc aagcataaat tgatgggtcc ggtgcatagg ttataaaaga attgggattt 600 gtgcatttca tgacggtgca atcagcttct gacgatttct ttttacacat atttttagaa 660 tgatcatata catagttagg tgggcattcg aaaacctgac ctttaccatc tttgcagaat 720 aagtatcttg tgcagtcttt cggatctggc tgatatccta caacggcgca tgtagtttct 780 gccgtcttac agttttcccc aggttttgat gagcacatat catctacaca aaatggagca 840 tcggaatttg agtctctgca attgattgtt gtaattgggg tttcttgtcc agcgcacaac 900 ttcaaagtag aacaatcgtg acaaaatgtt cccaactgct tattagtaca ctttatttct 960 tcattgccaa atgttgttcg tgctctggat cttcccactg ggaaaccgcg gacatatgtg 1020 ggcgaattcc tgtcagcagc cacagcaccc aaggcaaaca ctgcaaccaa taaagctcct 1080 aagaacttca ttgtga 1096 4 816 DNA Ctenocephalides felis 4 atgaagttct taggagcttt attggttgca gtgtttgcct tgggtgctgt ggctgctgac 60 aggaattcgc ccacatatgt ccgcggtttc ccagtgggaa gatccagagc acgaacaaca 120 tttggcaatg aagaaataaa gtgtactaat aagcagttgg gaacattttg tcacgattgt 180 tctactttga agttgtgcgc tggacaagaa accccaatta caacaatcaa ttgcagagac 240 tcaaattccg atgctccatt ttgtgtagat gatatgtgct catcaaaacc tggggaaaac 300 tgtaagacgg cagaaactac atgcgccgtt gtaggatatc agccagatcc gaaagactgc 360 acaagatact tattctgcaa agatggtaaa ggtcaggttt tcgaatgccc acctaactat 420 gtatatgatc attctaaaaa tatgtgtaaa aagaaatcgt cagaagctga ttgcaccgtc 480 atgaaatgca caaatcccaa ttcttttata acctatgcac cggacccatc aatttatgct 540 tggtgcaatg acaaattgca accgatcgta ctgaaatgtg aagacgacgt caacgaatgg 600 tttgacccaa aatctttctc gtgcagaact gcatgcaaaa gtgaaaacgt tttttccgat 660 cgaagagatt gtaaaaaata ttatcaatgt ttcttggtta acaacaaatg gcaaataaaa 720 cattatgatt gtccaaatgg cttgcacttt gataaaacgg agttgcgatg catacccacg 780 ccacccggcg aagaatgcaa aagtgagatt gctaag 816 5 816 DNA Ctenocephalides felis 5 cttagcaatc tcacttttgc attcttcgcc gggtggcgtg ggtatgcatc gcaactccgt 60 tttatcaaag tgcaagccat ttggacaatc ataatgtttt atttgccatt tgttgttaac 120 caagaaacat tgataatatt ttttacaatc tcttcgatcg gaaaaaacgt tttcactttt 180 gcatgcagtt ctgcacgaga aagattttgg gtcaaaccat tcgttgacgt cgtcttcaca 240 tttcagtacg atcggttgca atttgtcatt gcaccaagca taaattgatg ggtccggtgc 300 ataggttata aaagaattgg gatttgtgca tttcatgacg gtgcaatcag cttctgacga 360 tttcttttta cacatatttt tagaatgatc atatacatag ttaggtgggc attcgaaaac 420 ctgaccttta ccatctttgc agaataagta tcttgtgcag tctttcggat ctggctgata 480 tcctacaacg gcgcatgtag tttctgccgt cttacagttt tccccaggtt ttgatgagca 540 catatcatct acacaaaatg gagcatcgga atttgagtct ctgcaattga ttgttgtaat 600 tggggtttct tgtccagcgc acaacttcaa agtagaacaa tcgtgacaaa atgttcccaa 660 ctgcttatta gtacacttta tttcttcatt gccaaatgtt gttcgtgctc tggatcttcc 720 cactgggaaa ccgcggacat atgtgggcga attcctgtca gcagccacag cacccaaggc 780 aaacactgca accaataaag ctcctaagaa cttcat 816 6 445 DNA Ctenocephalides felis 6 gcaaacaaca aggtccgctg atgcaagatc cacacgattg tcacgcatat tacacatgtc 60 tagaaattgg atcattaccg aaacatttta attgtaataa aggtgcttat ttcaatacag 120 tcaaattaaa atgcgtgaaa ggaaattgcg aaaatagcac agaaattcct cttcctgagc 180 ttccagacat ttgcgatgaa gtaggacctt tggtgcaaga tccaaacgat tgccgcaagt 240 attattcatg cgtcacgatt ggaaaagaac ctgaacattt tacgtgcaat aaaggggcgt 300 attttgatcg agaaagatta cggtgtgtca gaggatcttg ttaacaaata ttgttatata 360 acaaagttca atctttaatt attatttaga agaatttgaa aatgtatatt taatgttttt 420 taataaaata gtttattggc aattt 445 7 1279 DNA Ctenocephalides felis 7 gattagctgc ccactatagg gctaaagcgg ccgccgggtg gtgttctgca gatccaagcc 60 catgtttctc ggaaggttct ataacttgca ccggtccagg agtttttcct gatccgtatg 120 attgtcagcg ttatcatgaa tgtaaaactg caaatgaatc atctaagcct gtcgagtgtg 180 ggggttacaa ggcttataat gttatagaaa ataattgtag cctgaacatg aatcatcaat 240 cgtgtaaacg cttacaattt cattgtgata ctataggaga tgaaaatgct tggccgagca 300 atagaaatat atattatagg tgcaccgaaa aaaccttgtg gttcaatagc aacaaaatat 360 tatatccttt attatatcgg tgtgatgaga gtgagatata tgatgcagtg cagagagttt 420 gcgtaagaga tgaaaccacc acgacgcctg ccacaacgcc aaccgaatct tccacgtcta 480 gtgaaacaac cacgacgtct gccacaacat caaccgaatc ttccacgtct agtgaaacaa 540 ccacgacgtc tgccacaaca ccaaccgaat cttccacgtc tagtgaaaca accacgacgt 600 ctgccacaac accaaccgaa tcttccacgt ctagtgaaac aaccacgacg tctgccacaa 660 caccaaccga atcttccacg tctagtgaaa caaccacgac gtctgccaca acaccaaccg 720 aatcttccac gtctggtgaa acaaccacga cgtctgccac aacaccaacc gaaccttcca 780 caaagcctac ttctacggaa actcccgcaa caaaaccacc gcaagaaata ccatgcaaac 840 aacaaggtcc gctgatgcaa gatccacacg attgtcacgc atattacaca tgtctagaaa 900 ttggatcatt accgaaacat tttaattgta ataaaggtgc ttatttcaat acagtcaaat 960 taaaatgcgt gaaaggaaat tgcgaaaata gcacagaaat tcctcttcct gagcttccag 1020 acatttgcga tgaagtagga cctttggtgc aagatccaaa cgattgccgc aagtattatt 1080 catgcgtcac gattggaaaa gaacctgaac attttacgtg caataaaggg gcgtattttg 1140 atcgagaaag attacggtgt gtcagaggat cttgttaaca aatattgtta tataacaaag 1200 ttcaatcttt aattattatt tagaagaatt tgaaaatgta tatttaatgt tttttaataa 1260 aatagtttat tggcaattt 1279 8 1279 DNA Ctenocephalides felis 8 aaattgccaa taaactattt tattaaaaaa cattaaatat acattttcaa attcttctaa 60 ataataatta aagattgaac tttgttatat aacaatattt gttaacaaga tcctctgaca 120 caccgtaatc tttctcgatc aaaatacgcc cctttattgc acgtaaaatg ttcaggttct 180 tttccaatcg tgacgcatga ataatacttg cggcaatcgt ttggatcttg caccaaaggt 240 cctacttcat cgcaaatgtc tggaagctca ggaagaggaa tttctgtgct attttcgcaa 300 tttcctttca cgcattttaa tttgactgta ttgaaataag cacctttatt acaattaaaa 360 tgtttcggta atgatccaat ttctagacat gtgtaatatg cgtgacaatc gtgtggatct 420 tgcatcagcg gaccttgttg tttgcatggt atttcttgcg gtggttttgt tgcgggagtt 480 tccgtagaag taggctttgt ggaaggttcg gttggtgttg tggcagacgt cgtggttgtt 540 tcaccagacg tggaagattc ggttggtgtt gtggcagacg tcgtggttgt ttcactagac 600 gtggaagatt cggttggtgt tgtggcagac gtcgtggttg tttcactaga cgtggaagat 660 tcggttggtg ttgtggcaga cgtcgtggtt gtttcactag acgtggaaga ttcggttggt 720 gttgtggcag acgtcgtggt tgtttcacta gacgtggaag attcggttga tgttgtggca 780 gacgtcgtgg ttgtttcact agacgtggaa gattcggttg gcgttgtggc aggcgtcgtg 840 gtggtttcat ctcttacgca aactctctgc actgcatcat atatctcact ctcatcacac 900 cgatataata aaggatataa tattttgttg ctattgaacc acaaggtttt ttcggtgcac 960 ctataatata tatttctatt gctcggccaa gcattttcat ctcctatagt atcacaatga 1020 aattgtaagc gtttacacga ttgatgattc atgttcaggc tacaattatt ttctataaca 1080 ttataagcct tgtaaccccc acactcgaca ggcttagatg attcatttgc agttttacat 1140 tcatgataac gctgacaatc atacggatca ggaaaaactc ctggaccggt gcaagttata 1200 gaaccttccg agaaacatgg gcttggatct gcagaacacc acccggcggc cgctttagcc 1260 ctatagtggg cagctaatc 1279 9 279 DNA Ctenocephalides felis 9 caatgatcaa gtcagttata attgtggcca acttttggct attcgtagcc ttgcaatcga 60 ttgataatcg gcagactaac aactgctctg agattggtgt tgatggtttt ttttgtctca 120 actgcagcgt cacagctttt tgtggtagag gacctacggg tgaattcaac accgtgtcta 180 caagtccatg tagttctggt gaagtttgca gtacctgggc gggtagatgt tctgcagatc 240 caagcccatg tttctcggaa ggttctataa cttgcaccg 279 10 279 DNA Ctenocephalides felis 10 cggtgcaagt tatagaacct tccgagaaac atgggcttgg atctgcagaa catctacccg 60 cccaggtact gcaaacttca ccagaactac atggacttgt agacacggtg ttgaattcac 120 ccgtaggtcc tctaccacaa aaagctgtga cgctgcagtt gagacaaaaa aaaccatcaa 180 caccaatctc agagcagttg ttagtctgcc gattatcaat cgattgcaag gctacgaata 240 gccaaaagtt ggccacaatt ataactgact tgatcattg 279 11 1465 DNA Ctenocephalides felis CDS (3)..(1361) 11 ca atg atc aag tca gtt ata att gtg gcc aac ttt tgg cta ttc gta 47 Met Ile Lys Ser Val Ile Ile Val Ala Asn Phe Trp Leu Phe Val 1 5 10 15 gcc ttg caa tcg att gat aat cgg cag act aac aac tgc tct gag att 95 Ala Leu Gln Ser Ile Asp Asn Arg Gln Thr Asn Asn Cys Ser Glu Ile 20 25 30 ggt gtt gat ggt ttt ttt tgt ctc aac tgc agc gtc aca gct ttt tgt 143 Gly Val Asp Gly Phe Phe Cys Leu Asn Cys Ser Val Thr Ala Phe Cys 35 40 45 ggt aga gga cct acg ggt gaa ttc aac acc gtg tct aca agt cca tgt 191 Gly Arg Gly Pro Thr Gly Glu Phe Asn Thr Val Ser Thr Ser Pro Cys 50 55 60 agt tct ggt gaa gtt tgc agt acc tgg gcg ggt aga tgt tct gca gat 239 Ser Ser Gly Glu Val Cys Ser Thr Trp Ala Gly Arg Cys Ser Ala Asp 65 70 75 cca agc cca tgt ttc tcg gaa ggt tct ata act tgc acc ggt cca gga 287 Pro Ser Pro Cys Phe Ser Glu Gly Ser Ile Thr Cys Thr Gly Pro Gly 80 85 90 95 gtt ttt cct gat ccg tat gat tgt cag cgt tat cat gaa tgt aaa act 335 Val Phe Pro Asp Pro Tyr Asp Cys Gln Arg Tyr His Glu Cys Lys Thr 100 105 110 gca aat gaa tca tct aag cct gtc gag tgt ggg ggt tac aag gct tat 383 Ala Asn Glu Ser Ser Lys Pro Val Glu Cys Gly Gly Tyr Lys Ala Tyr 115 120 125 aat gtt ata gaa aat aat tgt agc ctg aac atg aat cat caa tcg tgt 431 Asn Val Ile Glu Asn Asn Cys Ser Leu Asn Met Asn His Gln Ser Cys 130 135 140 aaa cgc tta caa ttt cat tgt gat act ata gga gat gaa aat gct tgg 479 Lys Arg Leu Gln Phe His Cys Asp Thr Ile Gly Asp Glu Asn Ala Trp 145 150 155 ccg agc aat aga aat ata tat tat agg tgc acc gaa aaa acc ttg tgg 527 Pro Ser Asn Arg Asn Ile Tyr Tyr Arg Cys Thr Glu Lys Thr Leu Trp 160 165 170 175 ttc aat agc aac aaa ata tta tat cct tta tta tat cgg tgt gat gag 575 Phe Asn Ser Asn Lys Ile Leu Tyr Pro Leu Leu Tyr Arg Cys Asp Glu 180 185 190 agt gag ata tat gat gca gtg cag aga gtt tgc gta aga gat gaa acc 623 Ser Glu Ile Tyr Asp Ala Val Gln Arg Val Cys Val Arg Asp Glu Thr 195 200 205 acc acg acg cct gcc aca acg cca acc gaa tct tcc acg tct agt gaa 671 Thr Thr Thr Pro Ala Thr Thr Pro Thr Glu Ser Ser Thr Ser Ser Glu 210 215 220 aca acc acg acg tct gcc aca aca tca acc gaa tct tcc acg tct agt 719 Thr Thr Thr Thr Ser Ala Thr Thr Ser Thr Glu Ser Ser Thr Ser Ser 225 230 235 gaa aca acc acg acg tct gcc aca aca cca acc gaa tct tcc acg tct 767 Glu Thr Thr Thr Thr Ser Ala Thr Thr Pro Thr Glu Ser Ser Thr Ser 240 245 250 255 agt gaa aca acc acg acg tct gcc aca aca cca acc gaa tct tcc acg 815 Ser Glu Thr Thr Thr Thr Ser Ala Thr Thr Pro Thr Glu Ser Ser Thr 260 265 270 tct agt gaa aca acc acg acg tct gcc aca aca cca acc gaa tct tcc 863 Ser Ser Glu Thr Thr Thr Thr Ser Ala Thr Thr Pro Thr Glu Ser Ser 275 280 285 acg tct agt gaa aca acc acg acg tct gcc aca aca cca acc gaa tct 911 Thr Ser Ser Glu Thr Thr Thr Thr Ser Ala Thr Thr Pro Thr Glu Ser 290 295 300 tcc acg tct ggt gaa aca acc acg acg tct gcc aca aca cca acc gaa 959 Ser Thr Ser Gly Glu Thr Thr Thr Thr Ser Ala Thr Thr Pro Thr Glu 305 310 315 cct tcc aca aag cct act tct acg gaa act ccc gca aca aaa cca ccg 1007 Pro Ser Thr Lys Pro Thr Ser Thr Glu Thr Pro Ala Thr Lys Pro Pro 320 325 330 335 caa gaa ata cca tgc aaa caa caa ggt ccg ctg atg caa gat cca cac 1055 Gln Glu Ile Pro Cys Lys Gln Gln Gly Pro Leu Met Gln Asp Pro His 340 345 350 gat tgt cac gca tat tac aca tgt cta gaa att gga tca tta ccg aaa 1103 Asp Cys His Ala Tyr Tyr Thr Cys Leu Glu Ile Gly Ser Leu Pro Lys 355 360 365 cat ttt aat tgt aat aaa ggt gct tat ttc aat aca gtc aaa tta aaa 1151 His Phe Asn Cys Asn Lys Gly Ala Tyr Phe Asn Thr Val Lys Leu Lys 370 375 380 tgc gtg aaa gga aat tgc gaa aat agc aca gaa att cct ctt cct gag 1199 Cys Val Lys Gly Asn Cys Glu Asn Ser Thr Glu Ile Pro Leu Pro Glu 385 390 395 ctt cca gac att tgc gat gaa gta gga cct ttg gtg caa gat cca aac 1247 Leu Pro Asp Ile Cys Asp Glu Val Gly Pro Leu Val Gln Asp Pro Asn 400 405 410 415 gat tgc cgc aag tat tat tca tgc gtc acg att gga aaa gaa cct gaa 1295 Asp Cys Arg Lys Tyr Tyr Ser Cys Val Thr Ile Gly Lys Glu Pro Glu 420 425 430 cat ttt acg tgc aat aaa ggg gcg tat ttt gat cga gaa aga tta cgg 1343 His Phe Thr Cys Asn Lys Gly Ala Tyr Phe Asp Arg Glu Arg Leu Arg 435 440 445 tgt gtc aga gga tct tgt taacaaatat tgttatataa caaagttcaa 1391 Cys Val Arg Gly Ser Cys 450 tctttaatta ttatttagaa gaatttgaaa atgtatattt aatgtttttt aataaaatag 1451 tttattggca attt 1465 12 453 PRT Ctenocephalides felis 12 Met Ile Lys Ser Val Ile Ile Val Ala Asn Phe Trp Leu Phe Val Ala 1 5 10 15 Leu Gln Ser Ile Asp Asn Arg Gln Thr Asn Asn Cys Ser Glu Ile Gly 20 25 30 Val Asp Gly Phe Phe Cys Leu Asn Cys Ser Val Thr Ala Phe Cys Gly 35 40 45 Arg Gly Pro Thr Gly Glu Phe Asn Thr Val Ser Thr Ser Pro Cys Ser 50 55 60 Ser Gly Glu Val Cys Ser Thr Trp Ala Gly Arg Cys Ser Ala Asp Pro 65 70 75 80 Ser Pro Cys Phe Ser Glu Gly Ser Ile Thr Cys Thr Gly Pro Gly Val 85 90 95 Phe Pro Asp Pro Tyr Asp Cys Gln Arg Tyr His Glu Cys Lys Thr Ala 100 105 110 Asn Glu Ser Ser Lys Pro Val Glu Cys Gly Gly Tyr Lys Ala Tyr Asn 115 120 125 Val Ile Glu Asn Asn Cys Ser Leu Asn Met Asn His Gln Ser Cys Lys 130 135 140 Arg Leu Gln Phe His Cys Asp Thr Ile Gly Asp Glu Asn Ala Trp Pro 145 150 155 160 Ser Asn Arg Asn Ile Tyr Tyr Arg Cys Thr Glu Lys Thr Leu Trp Phe 165 170 175 Asn Ser Asn Lys Ile Leu Tyr Pro Leu Leu Tyr Arg Cys Asp Glu Ser 180 185 190 Glu Ile Tyr Asp Ala Val Gln Arg Val Cys Val Arg Asp Glu Thr Thr 195 200 205 Thr Thr Pro Ala Thr Thr Pro Thr Glu Ser Ser Thr Ser Ser Glu Thr 210 215 220 Thr Thr Thr Ser Ala Thr Thr Ser Thr Glu Ser Ser Thr Ser Ser Glu 225 230 235 240 Thr Thr Thr Thr Ser Ala Thr Thr Pro Thr Glu Ser Ser Thr Ser Ser 245 250 255 Glu Thr Thr Thr Thr Ser Ala Thr Thr Pro Thr Glu Ser Ser Thr Ser 260 265 270 Ser Glu Thr Thr Thr Thr Ser Ala Thr Thr Pro Thr Glu Ser Ser Thr 275 280 285 Ser Ser Glu Thr Thr Thr Thr Ser Ala Thr Thr Pro Thr Glu Ser Ser 290 295 300 Thr Ser Gly Glu Thr Thr Thr Thr Ser Ala Thr Thr Pro Thr Glu Pro 305 310 315 320 Ser Thr Lys Pro Thr Ser Thr Glu Thr Pro Ala Thr Lys Pro Pro Gln 325 330 335 Glu Ile Pro Cys Lys Gln Gln Gly Pro Leu Met Gln Asp Pro His Asp 340 345 350 Cys His Ala Tyr Tyr Thr Cys Leu Glu Ile Gly Ser Leu Pro Lys His 355 360 365 Phe Asn Cys Asn Lys Gly Ala Tyr Phe Asn Thr Val Lys Leu Lys Cys 370 375 380 Val Lys Gly Asn Cys Glu Asn Ser Thr Glu Ile Pro Leu Pro Glu Leu 385 390 395 400 Pro Asp Ile Cys Asp Glu Val Gly Pro Leu Val Gln Asp Pro Asn Asp 405 410 415 Cys Arg Lys Tyr Tyr Ser Cys Val Thr Ile Gly Lys Glu Pro Glu His 420 425 430 Phe Thr Cys Asn Lys Gly Ala Tyr Phe Asp Arg Glu Arg Leu Arg Cys 435 440 445 Val Arg Gly Ser Cys 450 13 1465 DNA Ctenocephalides felis 13 aaattgccaa taaactattt tattaaaaaa cattaaatat acattttcaa attcttctaa 60 ataataatta aagattgaac tttgttatat aacaatattt gttaacaaga tcctctgaca 120 caccgtaatc tttctcgatc aaaatacgcc cctttattgc acgtaaaatg ttcaggttct 180 tttccaatcg tgacgcatga ataatacttg cggcaatcgt ttggatcttg caccaaaggt 240 cctacttcat cgcaaatgtc tggaagctca ggaagaggaa tttctgtgct attttcgcaa 300 tttcctttca cgcattttaa tttgactgta ttgaaataag cacctttatt acaattaaaa 360 tgtttcggta atgatccaat ttctagacat gtgtaatatg cgtgacaatc gtgtggatct 420 tgcatcagcg gaccttgttg tttgcatggt atttcttgcg gtggttttgt tgcgggagtt 480 tccgtagaag taggctttgt ggaaggttcg gttggtgttg tggcagacgt cgtggttgtt 540 tcaccagacg tggaagattc ggttggtgtt gtggcagacg tcgtggttgt ttcactagac 600 gtggaagatt cggttggtgt tgtggcagac gtcgtggttg tttcactaga cgtggaagat 660 tcggttggtg ttgtggcaga cgtcgtggtt gtttcactag acgtggaaga ttcggttggt 720 gttgtggcag acgtcgtggt tgtttcacta gacgtggaag attcggttga tgttgtggca 780 gacgtcgtgg ttgtttcact agacgtggaa gattcggttg gcgttgtggc aggcgtcgtg 840 gtggtttcat ctcttacgca aactctctgc actgcatcat atatctcact ctcatcacac 900 cgatataata aaggatataa tattttgttg ctattgaacc acaaggtttt ttcggtgcac 960 ctataatata tatttctatt gctcggccaa gcattttcat ctcctatagt atcacaatga 1020 aattgtaagc gtttacacga ttgatgattc atgttcaggc tacaattatt ttctataaca 1080 ttataagcct tgtaaccccc acactcgaca ggcttagatg attcatttgc agttttacat 1140 tcatgataac gctgacaatc atacggatca ggaaaaactc ctggaccggt gcaagttata 1200 gaaccttccg agaaacatgg gcttggatct gcagaacatc tacccgccca ggtactgcaa 1260 acttcaccag aactacatgg acttgtagac acggtgttga attcacccgt aggtcctcta 1320 ccacaaaaag ctgtgacgct gcagttgaga caaaaaaaac catcaacacc aatctcagag 1380 cagttgttag tctgccgatt atcaatcgat tgcaaggcta cgaatagcca aaagttggcc 1440 acaattataa ctgacttgat cattg 1465 14 1359 DNA Ctenocephalides felis 14 atgatcaagt cagttataat tgtggccaac ttttggctat tcgtagcctt gcaatcgatt 60 gataatcggc agactaacaa ctgctctgag attggtgttg atggtttttt ttgtctcaac 120 tgcagcgtca cagctttttg tggtagagga cctacgggtg aattcaacac cgtgtctaca 180 agtccatgta gttctggtga agtttgcagt acctgggcgg gtagatgttc tgcagatcca 240 agcccatgtt tctcggaagg ttctataact tgcaccggtc caggagtttt tcctgatccg 300 tatgattgtc agcgttatca tgaatgtaaa actgcaaatg aatcatctaa gcctgtcgag 360 tgtgggggtt acaaggctta taatgttata gaaaataatt gtagcctgaa catgaatcat 420 caatcgtgta aacgcttaca atttcattgt gatactatag gagatgaaaa tgcttggccg 480 agcaatagaa atatatatta taggtgcacc gaaaaaacct tgtggttcaa tagcaacaaa 540 atattatatc ctttattata tcggtgtgat gagagtgaga tatatgatgc agtgcagaga 600 gtttgcgtaa gagatgaaac caccacgacg cctgccacaa cgccaaccga atcttccacg 660 tctagtgaaa caaccacgac gtctgccaca acatcaaccg aatcttccac gtctagtgaa 720 acaaccacga cgtctgccac aacaccaacc gaatcttcca cgtctagtga aacaaccacg 780 acgtctgcca caacaccaac cgaatcttcc acgtctagtg aaacaaccac gacgtctgcc 840 acaacaccaa ccgaatcttc cacgtctagt gaaacaacca cgacgtctgc cacaacacca 900 accgaatctt ccacgtctgg tgaaacaacc acgacgtctg ccacaacacc aaccgaacct 960 tccacaaagc ctacttctac ggaaactccc gcaacaaaac caccgcaaga aataccatgc 1020 aaacaacaag gtccgctgat gcaagatcca cacgattgtc acgcatatta cacatgtcta 1080 gaaattggat cattaccgaa acattttaat tgtaataaag gtgcttattt caatacagtc 1140 aaattaaaat gcgtgaaagg aaattgcgaa aatagcacag aaattcctct tcctgagctt 1200 ccagacattt gcgatgaagt aggacctttg gtgcaagatc caaacgattg ccgcaagtat 1260 tattcatgcg tcacgattgg aaaagaacct gaacatttta cgtgcaataa aggggcgtat 1320 tttgatcgag aaagattacg gtgtgtcaga ggatcttgt 1359 15 1359 DNA Ctenocephalides felis 15 acaagatcct ctgacacacc gtaatctttc tcgatcaaaa tacgcccctt tattgcacgt 60 aaaatgttca ggttcttttc caatcgtgac gcatgaataa tacttgcggc aatcgtttgg 120 atcttgcacc aaaggtccta cttcatcgca aatgtctgga agctcaggaa gaggaatttc 180 tgtgctattt tcgcaatttc ctttcacgca ttttaatttg actgtattga aataagcacc 240 tttattacaa ttaaaatgtt tcggtaatga tccaatttct agacatgtgt aatatgcgtg 300 acaatcgtgt ggatcttgca tcagcggacc ttgttgtttg catggtattt cttgcggtgg 360 ttttgttgcg ggagtttccg tagaagtagg ctttgtggaa ggttcggttg gtgttgtggc 420 agacgtcgtg gttgtttcac cagacgtgga agattcggtt ggtgttgtgg cagacgtcgt 480 ggttgtttca ctagacgtgg aagattcggt tggtgttgtg gcagacgtcg tggttgtttc 540 actagacgtg gaagattcgg ttggtgttgt ggcagacgtc gtggttgttt cactagacgt 600 ggaagattcg gttggtgttg tggcagacgt cgtggttgtt tcactagacg tggaagattc 660 ggttgatgtt gtggcagacg tcgtggttgt ttcactagac gtggaagatt cggttggcgt 720 tgtggcaggc gtcgtggtgg tttcatctct tacgcaaact ctctgcactg catcatatat 780 ctcactctca tcacaccgat ataataaagg atataatatt ttgttgctat tgaaccacaa 840 ggttttttcg gtgcacctat aatatatatt tctattgctc ggccaagcat tttcatctcc 900 tatagtatca caatgaaatt gtaagcgttt acacgattga tgattcatgt tcaggctaca 960 attattttct ataacattat aagccttgta acccccacac tcgacaggct tagatgattc 1020 atttgcagtt ttacattcat gataacgctg acaatcatac ggatcaggaa aaactcctgg 1080 accggtgcaa gttatagaac cttccgagaa acatgggctt ggatctgcag aacatctacc 1140 cgcccaggta ctgcaaactt caccagaact acatggactt gtagacacgg tgttgaattc 1200 acccgtaggt cctctaccac aaaaagctgt gacgctgcag ttgagacaaa aaaaaccatc 1260 aacaccaatc tcagagcagt tgttagtctg ccgattatca atcgattgca aggctacgaa 1320 tagccaaaag ttggccacaa ttataactga cttgatcat 1359 16 387 DNA Ctenocephalides felis CDS (20)..(262) 16 gttaatttaa aataacaaa atg aaa gga aca tta tta ata tta tca tgt ctt 52 Met Lys Gly Thr Leu Leu Ile Leu Ser Cys Leu 1 5 10 gtg atc atg ata agt gcc gaa tat gct gac gta gat gtg tgc caa gat 100 Val Ile Met Ile Ser Ala Glu Tyr Ala Asp Val Asp Val Cys Gln Asp 15 20 25 ttg gac gat gga act ttt ctt gct gat tca aac aat tgc caa aat ttc 148 Leu Asp Asp Gly Thr Phe Leu Ala Asp Ser Asn Asn Cys Gln Asn Phe 30 35 40 ttc att tgt gat gga ggc cga gct tgg aaa atg tat tgt cca gga tca 196 Phe Ile Cys Asp Gly Gly Arg Ala Trp Lys Met Tyr Cys Pro Gly Ser 45 50 55 ctt tta tgg aat gat cac gaa gga aca tgt gat tac gca caa aat gta 244 Leu Leu Trp Asn Asp His Glu Gly Thr Cys Asp Tyr Ala Gln Asn Val 60 65 70 75 gaa tgt tac caa cca gaa taaaacattt taatatctga cagcgatttt 292 Glu Cys Tyr Gln Pro Glu 80 ctgaaactat atttcatact actgttataa taaatttatc ttcattgctc tcctcctata 352 aatttattcc gttttaataa aatcaatata aagac 387 17 81 PRT Ctenocephalides felis 17 Met Lys Gly Thr Leu Leu Ile Leu Ser Cys Leu Val Ile Met Ile Ser 1 5 10 15 Ala Glu Tyr Ala Asp Val Asp Val Cys Gln Asp Leu Asp Asp Gly Thr 20 25 30 Phe Leu Ala Asp Ser Asn Asn Cys Gln Asn Phe Phe Ile Cys Asp Gly 35 40 45 Gly Arg Ala Trp Lys Met Tyr Cys Pro Gly Ser Leu Leu Trp Asn Asp 50 55 60 His Glu Gly Thr Cys Asp Tyr Ala Gln Asn Val Glu Cys Tyr Gln Pro 65 70 75 80 Glu 18 387 DNA Ctenocephalides felis 18 gtctttatat tgattttatt aaaacggaat aaatttatag gaggagagca atgaagataa 60 atttattata acagtagtat gaaatatagt ttcagaaaat cgctgtcaga tattaaaatg 120 ttttattctg gttggtaaca ttctacattt tgtgcgtaat cacatgttcc ttcgtgatca 180 ttccataaaa gtgatcctgg acaatacatt ttccaagctc ggcctccatc acaaatgaag 240 aaattttggc aattgtttga atcagcaaga aaagttccat cgtccaaatc ttggcacaca 300 tctacgtcag catattcggc acttatcatg atcacaagac atgataatat taataatgtt 360 cctttcattt tgttatttta aattaac 387 19 243 DNA Ctenocephalides felis 19 atgaaaggaa cattattaat attatcatgt cttgtgatca tgataagtgc cgaatatgct 60 gacgtagatg tgtgccaaga tttggacgat ggaacttttc ttgctgattc aaacaattgc 120 caaaatttct tcatttgtga tggaggccga gcttggaaaa tgtattgtcc aggatcactt 180 ttatggaatg atcacgaagg aacatgtgat tacgcacaaa atgtagaatg ttaccaacca 240 gaa 243 20 243 DNA Ctenocephalides felis 20 ttctggttgg taacattcta cattttgtgc gtaatcacat gttccttcgt gatcattcca 60 taaaagtgat cctggacaat acattttcca agctcggcct ccatcacaaa tgaagaaatt 120 ttggcaattg tttgaatcag caagaaaagt tccatcgtcc aaatcttggc acacatctac 180 gtcagcatat tcggcactta tcatgatcac aagacatgat aatattaata atgttccttt 240 cat 243 21 960 DNA Ctenocephalides felis 21 ttaatgtcga cggaacgcct ttaacagtaa ataaagaagt atttgcatca ttggatgagc 60 ccgcaccagg agtagtacct actcctgaac ctacacctgt accgaaaccc gagcaaaaat 120 gtaaaaaagt aaaatttagt tgcgtgaatt cgtgcagttc acccgaaatg cagtattgtc 180 cggaaatagg agcagatccg gttaaggaat cctgtagccc agatcaagtg tgcgctgatc 240 aaagtggata tctacagtgc accactaaag aaagtacagt ctgcaaagta caaggtttca 300 aatgtccgtc accatcgaga ttttatccaa atataaatga ttgtcaaagc tattattatt 360 gtgacgaaaa tagtatagga acccaatatt attgccccgc aaattttgca tatgatccgt 420 tacgtcataa ttgcggacct atggctctgg gcacaaaatg ctatacagtt acatgtcctg 480 cacagcctaa ggtgcttccg tacattggtg ataaatcatt gtacgtcgta tgtatggccg 540 gaagaggaac cgtattgcaa tgcgaagaac ccgccgagtt ttccccaagg agcgaaacct 600 gtgtcgggca atgccgagca cgtggaaaat ttgctttcaa gaacgacgca acatgccgga 660 agttcttcac gtgtttacgt cctaaaggag agccagttcc tgatcaatgt ccgattggaa 720 cagtatttaa ccaagctact caaagctgca acacaggaac ttgcgagagg aaacctaaat 780 tatattaata tattgatgaa gtattcaaca aaagaaacta tacaaaatat gtactttgtt 840 ttactttatg tgttatataa aaaaatatta tggttgaaca caggctcgca aatatgataa 900 ggcatttaag aattttacaa tttagatttt tttaaatcca tgaatatatt tgttctaatc 960 22 960 DNA Ctenocephalides felis 22 gattagaaca aatatattca tggatttaaa aaaatctaaa ttgtaaaatt cttaaatgcc 60 ttatcatatt tgcgagcctg tgttcaacca taatattttt ttatataaca cataaagtaa 120 aacaaagtac atattttgta tagtttcttt tgttgaatac ttcatcaata tattaatata 180 atttaggttt cctctcgcaa gttcctgtgt tgcagctttg agtagcttgg ttaaatactg 240 ttccaatcgg acattgatca ggaactggct ctcctttagg acgtaaacac gtgaagaact 300 tccggcatgt tgcgtcgttc ttgaaagcaa attttccacg tgctcggcat tgcccgacac 360 aggtttcgct ccttggggaa aactcggcgg gttcttcgca ttgcaatacg gttcctcttc 420 cggccataca tacgacgtac aatgatttat caccaatgta cggaagcacc ttaggctgtg 480 caggacatgt aactgtatag cattttgtgc ccagagccat aggtccgcaa ttatgacgta 540 acggatcata tgcaaaattt gcggggcaat aatattgggt tcctatacta ttttcgtcac 600 aataataata gctttgacaa tcatttatat ttggataaaa tctcgatggt gacggacatt 660 tgaaaccttg tactttgcag actgtacttt ctttagtggt gcactgtaga tatccacttt 720 gatcagcgca cacttgatct gggctacagg attccttaac cggatctgct cctatttccg 780 gacaatactg catttcgggt gaactgcacg aattcacgca actaaatttt acttttttac 840 atttttgctc gggtttcggt acaggtgtag gttcaggagt aggtactact cctggtgcgg 900 gctcatccaa tgatgcaaat acttctttat ttactgttaa aggcgttccg tcgacattaa 960 23 1029 DNA Ctenocephalides felis 23 tgttttatat cacattggtt tttattagtt ttgtggcgtt atctgtcgtt accgcttatg 60 atggtgagtt taatgtcgac ggaacgcctt taacagtaaa taaagaagta tttgcatcat 120 tggatgagcc cgcaccagga gtagtaccta ctcctgaacc tacacctgta ccgaaacccg 180 agcaaaaatg taaaaaagta aaatttagtt gcgtgaattc gtgcagttca cccgaaatgc 240 agtattgtcc ggaaatagga gcagatccgg ttaaggaatc ctgtagccca gatcaagtgt 300 gcgctgatca aagtggatat ctacagtgca ccactaaaga aagtacagtc tgcaaagtac 360 aaggtttcaa atgtccgtca ccatcgagat tttatccaaa tataaatgat tgtcaaagct 420 attattattg tgacgaaaat agtataggaa cccaatatta ttgccccgca aattttgcat 480 atgatccgtt acgtcataat tgcggaccta tggctctggg cacaaaatgc tatacagtta 540 catgtcctgc acagcctaag gtgcttccgt acattggtga taaatcattg tacgtcgtat 600 gtatggccgg aagaggaacc gtattgcaat gcgaagaacc cgccgagttt tccccaagga 660 gcgaaacctg tgtcgggcaa tgccgagcac gtggaaaatt tgctttcaag aacgacgcaa 720 catgccggaa gttcttcacg tgtttacgtc ctaaaggaga gccagttcct gatcaatgtc 780 cgattggaac agtatttaac caagctactc aaagctgcaa cacaggaact tgcgagagga 840 aacctaaatt atattaatat attgatgaag tattcaacaa aagaaactat acaaaatatg 900 tactttgttt tactttatgt gttatataaa aaaatattat ggttgaacac aggctcgcaa 960 atatgataag gcatttaaga attttacaat ttagattttt ttaaatccat gaatatattt 1020 gttctaatc 1029 24 1029 DNA Ctenocephalides felis 24 gattagaaca aatatattca tggatttaaa aaaatctaaa ttgtaaaatt cttaaatgcc 60 ttatcatatt tgcgagcctg tgttcaacca taatattttt ttatataaca cataaagtaa 120 aacaaagtac atattttgta tagtttcttt tgttgaatac ttcatcaata tattaatata 180 atttaggttt cctctcgcaa gttcctgtgt tgcagctttg agtagcttgg ttaaatactg 240 ttccaatcgg acattgatca ggaactggct ctcctttagg acgtaaacac gtgaagaact 300 tccggcatgt tgcgtcgttc ttgaaagcaa attttccacg tgctcggcat tgcccgacac 360 aggtttcgct ccttggggaa aactcggcgg gttcttcgca ttgcaatacg gttcctcttc 420 cggccataca tacgacgtac aatgatttat caccaatgta cggaagcacc ttaggctgtg 480 caggacatgt aactgtatag cattttgtgc ccagagccat aggtccgcaa ttatgacgta 540 acggatcata tgcaaaattt gcggggcaat aatattgggt tcctatacta ttttcgtcac 600 aataataata gctttgacaa tcatttatat ttggataaaa tctcgatggt gacggacatt 660 tgaaaccttg tactttgcag actgtacttt ctttagtggt gcactgtaga tatccacttt 720 gatcagcgca cacttgatct gggctacagg attccttaac cggatctgct cctatttccg 780 gacaatactg catttcgggt gaactgcacg aattcacgca actaaatttt acttttttac 840 atttttgctc gggtttcggt acaggtgtag gttcaggagt aggtactact cctggtgcgg 900 gctcatccaa tgatgcaaat acttctttat ttactgttaa aggcgttccg tcgacattaa 960 actcaccatc ataagcggta acgacagata acgccacaaa actaataaaa accaatgtga 1020 tataaaaca 1029 25 1048 DNA Ctenocephalides felis CDS (19)..(873) 25 gtaacatatt tattaaga atg ttt tat atc aca ttg gtt ttt att agt ttt 51 Met Phe Tyr Ile Thr Leu Val Phe Ile Ser Phe 1 5 10 gtg gcg tta tct gtc gtt acc gct tat gat ggt gag ttt aat gtc gac 99 Val Ala Leu Ser Val Val Thr Ala Tyr Asp Gly Glu Phe Asn Val Asp 15 20 25 gga acg cct tta aca gta aat aaa gaa gta ttt gca tca ttg gat gag 147 Gly Thr Pro Leu Thr Val Asn Lys Glu Val Phe Ala Ser Leu Asp Glu 30 35 40 ccc gca cca gga gta gta cct act cct gaa cct aca cct gta ccg aaa 195 Pro Ala Pro Gly Val Val Pro Thr Pro Glu Pro Thr Pro Val Pro Lys 45 50 55 ccc gag caa aaa tgt aaa aaa gta aaa ttt agt tgc gtg aat tcg tgc 243 Pro Glu Gln Lys Cys Lys Lys Val Lys Phe Ser Cys Val Asn Ser Cys 60 65 70 75 agt tca ccc gaa atg cag tat tgt ccg gaa ata gga gca gat ccg gtt 291 Ser Ser Pro Glu Met Gln Tyr Cys Pro Glu Ile Gly Ala Asp Pro Val 80 85 90 aag gaa tcc tgt agc cca gat caa gtg tgc gct gat caa agt gga tat 339 Lys Glu Ser Cys Ser Pro Asp Gln Val Cys Ala Asp Gln Ser Gly Tyr 95 100 105 cta cag tgc acc act aaa gaa agt aca gtc tgc aaa gta caa ggt ttc 387 Leu Gln Cys Thr Thr Lys Glu Ser Thr Val Cys Lys Val Gln Gly Phe 110 115 120 aaa tgt ccg tca cca tcg aga ttt tat cca aat ata aat gat tgt caa 435 Lys Cys Pro Ser Pro Ser Arg Phe Tyr Pro Asn Ile Asn Asp Cys Gln 125 130 135 agc tat tat tat tgt gac gaa aat agt ata gga acc caa tat tat tgc 483 Ser Tyr Tyr Tyr Cys Asp Glu Asn Ser Ile Gly Thr Gln Tyr Tyr Cys 140 145 150 155 ccc gca aat ttt gca tat gat ccg tta cgt cat aat tgc gga cct atg 531 Pro Ala Asn Phe Ala Tyr Asp Pro Leu Arg His Asn Cys Gly Pro Met 160 165 170 gct ctg ggc aca aaa tgc tat aca gtt aca tgt cct gca cag cct aag 579 Ala Leu Gly Thr Lys Cys Tyr Thr Val Thr Cys Pro Ala Gln Pro Lys 175 180 185 gtg ctt ccg tac att ggt gat aaa tca ttg tac gtc gta tgt atg gcc 627 Val Leu Pro Tyr Ile Gly Asp Lys Ser Leu Tyr Val Val Cys Met Ala 190 195 200 gga aga gga acc gta ttg caa tgc gaa gaa ccc gcc gag ttt tcc cca 675 Gly Arg Gly Thr Val Leu Gln Cys Glu Glu Pro Ala Glu Phe Ser Pro 205 210 215 agg agc gaa acc tgt gtc ggg caa tgc cga gca cgt gga aaa ttt gct 723 Arg Ser Glu Thr Cys Val Gly Gln Cys Arg Ala Arg Gly Lys Phe Ala 220 225 230 235 ttc aag aac gac gca aca tgc cgg aag ttc ttc acg tgt tta cgt cct 771 Phe Lys Asn Asp Ala Thr Cys Arg Lys Phe Phe Thr Cys Leu Arg Pro 240 245 250 aaa gga gag cca gtt cct gat caa tgt ccg att gga aca gta ttt aac 819 Lys Gly Glu Pro Val Pro Asp Gln Cys Pro Ile Gly Thr Val Phe Asn 255 260 265 caa gct act caa agc tgc aac aca gga act tgc gag agg aaa cct aaa 867 Gln Ala Thr Gln Ser Cys Asn Thr Gly Thr Cys Glu Arg Lys Pro Lys 270 275 280 tta tat taatatattg atgaagtatt caacaaaaga aactatacaa aatatgtact 923 Leu Tyr 285 ttgttttact ttatgtgtta tataaaaaaa tattatggtt gaacacaggc tcgcaaatat 983 gataaggcat ttaagaattt tacaatttag atttttttaa atccatgaat atatttgttc 1043 taatc 1048 26 285 PRT Ctenocephalides felis 26 Met Phe Tyr Ile Thr Leu Val Phe Ile Ser Phe Val Ala Leu Ser Val 1 5 10 15 Val Thr Ala Tyr Asp Gly Glu Phe Asn Val Asp Gly Thr Pro Leu Thr 20 25 30 Val Asn Lys Glu Val Phe Ala Ser Leu Asp Glu Pro Ala Pro Gly Val 35 40 45 Val Pro Thr Pro Glu Pro Thr Pro Val Pro Lys Pro Glu Gln Lys Cys 50 55 60 Lys Lys Val Lys Phe Ser Cys Val Asn Ser Cys Ser Ser Pro Glu Met 65 70 75 80 Gln Tyr Cys Pro Glu Ile Gly Ala Asp Pro Val Lys Glu Ser Cys Ser 85 90 95 Pro Asp Gln Val Cys Ala Asp Gln Ser Gly Tyr Leu Gln Cys Thr Thr 100 105 110 Lys Glu Ser Thr Val Cys Lys Val Gln Gly Phe Lys Cys Pro Ser Pro 115 120 125 Ser Arg Phe Tyr Pro Asn Ile Asn Asp Cys Gln Ser Tyr Tyr Tyr Cys 130 135 140 Asp Glu Asn Ser Ile Gly Thr Gln Tyr Tyr Cys Pro Ala Asn Phe Ala 145 150 155 160 Tyr Asp Pro Leu Arg His Asn Cys Gly Pro Met Ala Leu Gly Thr Lys 165 170 175 Cys Tyr Thr Val Thr Cys Pro Ala Gln Pro Lys Val Leu Pro Tyr Ile 180 185 190 Gly Asp Lys Ser Leu Tyr Val Val Cys Met Ala Gly Arg Gly Thr Val 195 200 205 Leu Gln Cys Glu Glu Pro Ala Glu Phe Ser Pro Arg Ser Glu Thr Cys 210 215 220 Val Gly Gln Cys Arg Ala Arg Gly Lys Phe Ala Phe Lys Asn Asp Ala 225 230 235 240 Thr Cys Arg Lys Phe Phe Thr Cys Leu Arg Pro Lys Gly Glu Pro Val 245 250 255 Pro Asp Gln Cys Pro Ile Gly Thr Val Phe Asn Gln Ala Thr Gln Ser 260 265 270 Cys Asn Thr Gly Thr Cys Glu Arg Lys Pro Lys Leu Tyr 275 280 285 27 1048 DNA Ctenocephalides felis 27 gattagaaca aatatattca tggatttaaa aaaatctaaa ttgtaaaatt cttaaatgcc 60 ttatcatatt tgcgagcctg tgttcaacca taatattttt ttatataaca cataaagtaa 120 aacaaagtac atattttgta tagtttcttt tgttgaatac ttcatcaata tattaatata 180 atttaggttt cctctcgcaa gttcctgtgt tgcagctttg agtagcttgg ttaaatactg 240 ttccaatcgg acattgatca ggaactggct ctcctttagg acgtaaacac gtgaagaact 300 tccggcatgt tgcgtcgttc ttgaaagcaa attttccacg tgctcggcat tgcccgacac 360 aggtttcgct ccttggggaa aactcggcgg gttcttcgca ttgcaatacg gttcctcttc 420 cggccataca tacgacgtac aatgatttat caccaatgta cggaagcacc ttaggctgtg 480 caggacatgt aactgtatag cattttgtgc ccagagccat aggtccgcaa ttatgacgta 540 acggatcata tgcaaaattt gcggggcaat aatattgggt tcctatacta ttttcgtcac 600 aataataata gctttgacaa tcatttatat ttggataaaa tctcgatggt gacggacatt 660 tgaaaccttg tactttgcag actgtacttt ctttagtggt gcactgtaga tatccacttt 720 gatcagcgca cacttgatct gggctacagg attccttaac cggatctgct cctatttccg 780 gacaatactg catttcgggt gaactgcacg aattcacgca actaaatttt acttttttac 840 atttttgctc gggtttcggt acaggtgtag gttcaggagt aggtactact cctggtgcgg 900 gctcatccaa tgatgcaaat acttctttat ttactgttaa aggcgttccg tcgacattaa 960 actcaccatc ataagcggta acgacagata acgccacaaa actaataaaa accaatgtga 1020 tataaaacat tcttaataaa tatgttac 1048 28 855 DNA Ctenocephalides felis 28 atgttttata tcacattggt ttttattagt tttgtggcgt tatctgtcgt taccgcttat 60 gatggtgagt ttaatgtcga cggaacgcct ttaacagtaa ataaagaagt atttgcatca 120 ttggatgagc ccgcaccagg agtagtacct actcctgaac ctacacctgt accgaaaccc 180 gagcaaaaat gtaaaaaagt aaaatttagt tgcgtgaatt cgtgcagttc acccgaaatg 240 cagtattgtc cggaaatagg agcagatccg gttaaggaat cctgtagccc agatcaagtg 300 tgcgctgatc aaagtggata tctacagtgc accactaaag aaagtacagt ctgcaaagta 360 caaggtttca aatgtccgtc accatcgaga ttttatccaa atataaatga ttgtcaaagc 420 tattattatt gtgacgaaaa tagtatagga acccaatatt attgccccgc aaattttgca 480 tatgatccgt tacgtcataa ttgcggacct atggctctgg gcacaaaatg ctatacagtt 540 acatgtcctg cacagcctaa ggtgcttccg tacattggtg ataaatcatt gtacgtcgta 600 tgtatggccg gaagaggaac cgtattgcaa tgcgaagaac ccgccgagtt ttccccaagg 660 agcgaaacct gtgtcgggca atgccgagca cgtggaaaat ttgctttcaa gaacgacgca 720 acatgccgga agttcttcac gtgtttacgt cctaaaggag agccagttcc tgatcaatgt 780 ccgattggaa cagtatttaa ccaagctact caaagctgca acacaggaac ttgcgagagg 840 aaacctaaat tatat 855 29 855 DNA Ctenocephalides felis 29 atataattta ggtttcctct cgcaagttcc tgtgttgcag ctttgagtag cttggttaaa 60 tactgttcca atcggacatt gatcaggaac tggctctcct ttaggacgta aacacgtgaa 120 gaacttccgg catgttgcgt cgttcttgaa agcaaatttt ccacgtgctc ggcattgccc 180 gacacaggtt tcgctccttg gggaaaactc ggcgggttct tcgcattgca atacggttcc 240 tcttccggcc atacatacga cgtacaatga tttatcacca atgtacggaa gcaccttagg 300 ctgtgcagga catgtaactg tatagcattt tgtgcccaga gccataggtc cgcaattatg 360 acgtaacgga tcatatgcaa aatttgcggg gcaataatat tgggttccta tactattttc 420 gtcacaataa taatagcttt gacaatcatt tatatttgga taaaatctcg atggtgacgg 480 acatttgaaa ccttgtactt tgcagactgt actttcttta gtggtgcact gtagatatcc 540 actttgatca gcgcacactt gatctgggct acaggattcc ttaaccggat ctgctcctat 600 ttccggacaa tactgcattt cgggtgaact gcacgaattc acgcaactaa attttacttt 660 tttacatttt tgctcgggtt tcggtacagg tgtaggttca ggagtaggta ctactcctgg 720 tgcgggctca tccaatgatg caaatacttc tttatttact gttaaaggcg ttccgtcgac 780 attaaactca ccatcataag cggtaacgac agataacgcc acaaaactaa taaaaaccaa 840 tgtgatataa aacat 855 30 802 DNA Ctenocephalides felis CDS (2)..(799) 30 t tat gat ggt gag ttt aat gtc gac gga acg cct tta aca gta aat aaa 49 Tyr Asp Gly Glu Phe Asn Val Asp Gly Thr Pro Leu Thr Val Asn Lys 1 5 10 15 gaa gta ttt gca tca ttg gat gag ccc gca cca gga gta gta cct act 97 Glu Val Phe Ala Ser Leu Asp Glu Pro Ala Pro Gly Val Val Pro Thr 20 25 30 cct gaa cct aca cct gta ccg aaa ccc gag caa aaa tgt aaa aaa gta 145 Pro Glu Pro Thr Pro Val Pro Lys Pro Glu Gln Lys Cys Lys Lys Val 35 40 45 aaa ttt agt tgc gtg aat tcg tgc agt tca ccc gaa atg cag tat tgt 193 Lys Phe Ser Cys Val Asn Ser Cys Ser Ser Pro Glu Met Gln Tyr Cys 50 55 60 ccg gaa ata gga gca gat ccg gtt aag gaa tcc tgt agc cca gat caa 241 Pro Glu Ile Gly Ala Asp Pro Val Lys Glu Ser Cys Ser Pro Asp Gln 65 70 75 80 gtg tgc gct gat caa agt gga tat cta cag tgc acc act aaa gaa agt 289 Val Cys Ala Asp Gln Ser Gly Tyr Leu Gln Cys Thr Thr Lys Glu Ser 85 90 95 aca gtc tgc aaa gta caa ggt ttc aaa tgt ccg tca cca tcg aga ttt 337 Thr Val Cys Lys Val Gln Gly Phe Lys Cys Pro Ser Pro Ser Arg Phe 100 105 110 tat cca aat ata aat gat tgt caa agc tat tat tat tgt gac gaa aat 385 Tyr Pro Asn Ile Asn Asp Cys Gln Ser Tyr Tyr Tyr Cys Asp Glu Asn 115 120 125 agt ata gga acc caa tat tat tgc ccc gca aat ttt gca tat gat ccg 433 Ser Ile Gly Thr Gln Tyr Tyr Cys Pro Ala Asn Phe Ala Tyr Asp Pro 130 135 140 tta cgt cat aat tgc gga cct atg gct ctg ggc aca aaa tgc tat aca 481 Leu Arg His Asn Cys Gly Pro Met Ala Leu Gly Thr Lys Cys Tyr Thr 145 150 155 160 gtt aca tgt cct gca cag cct aag gtg ctt ccg tac att ggt gat aaa 529 Val Thr Cys Pro Ala Gln Pro Lys Val Leu Pro Tyr Ile Gly Asp Lys 165 170 175 tca ttg tac gtc gta tgt atg gcc gga aga gga acc gta ttg caa tgc 577 Ser Leu Tyr Val Val Cys Met Ala Gly Arg Gly Thr Val Leu Gln Cys 180 185 190 gaa gaa ccc gcc gag ttt tcc cca agg agc gaa acc tgt gtc ggg caa 625 Glu Glu Pro Ala Glu Phe Ser Pro Arg Ser Glu Thr Cys Val Gly Gln 195 200 205 tgc cga gca cgt gga aaa ttt gct ttc aag aac gac gca aca tgc cgg 673 Cys Arg Ala Arg Gly Lys Phe Ala Phe Lys Asn Asp Ala Thr Cys Arg 210 215 220 aag ttc ttc acg tgt tta cgt cct aaa gga gag cca gtt cct gat caa 721 Lys Phe Phe Thr Cys Leu Arg Pro Lys Gly Glu Pro Val Pro Asp Gln 225 230 235 240 tgt ccg att gga aca gta ttt aac caa gct act caa agc tgc aac aca 769 Cys Pro Ile Gly Thr Val Phe Asn Gln Ala Thr Gln Ser Cys Asn Thr 245 250 255 gga act tgc gag agg aaa cct aaa tta tat taa 802 Gly Thr Cys Glu Arg Lys Pro Lys Leu Tyr 260 265 31 266 PRT Ctenocephalides felis 31 Tyr Asp Gly Glu Phe Asn Val Asp Gly Thr Pro Leu Thr Val Asn Lys 1 5 10 15 Glu Val Phe Ala Ser Leu Asp Glu Pro Ala Pro Gly Val Val Pro Thr 20 25 30 Pro Glu Pro Thr Pro Val Pro Lys Pro Glu Gln Lys Cys Lys Lys Val 35 40 45 Lys Phe Ser Cys Val Asn Ser Cys Ser Ser Pro Glu Met Gln Tyr Cys 50 55 60 Pro Glu Ile Gly Ala Asp Pro Val Lys Glu Ser Cys Ser Pro Asp Gln 65 70 75 80 Val Cys Ala Asp Gln Ser Gly Tyr Leu Gln Cys Thr Thr Lys Glu Ser 85 90 95 Thr Val Cys Lys Val Gln Gly Phe Lys Cys Pro Ser Pro Ser Arg Phe 100 105 110 Tyr Pro Asn Ile Asn Asp Cys Gln Ser Tyr Tyr Tyr Cys Asp Glu Asn 115 120 125 Ser Ile Gly Thr Gln Tyr Tyr Cys Pro Ala Asn Phe Ala Tyr Asp Pro 130 135 140 Leu Arg His Asn Cys Gly Pro Met Ala Leu Gly Thr Lys Cys Tyr Thr 145 150 155 160 Val Thr Cys Pro Ala Gln Pro Lys Val Leu Pro Tyr Ile Gly Asp Lys 165 170 175 Ser Leu Tyr Val Val Cys Met Ala Gly Arg Gly Thr Val Leu Gln Cys 180 185 190 Glu Glu Pro Ala Glu Phe Ser Pro Arg Ser Glu Thr Cys Val Gly Gln 195 200 205 Cys Arg Ala Arg Gly Lys Phe Ala Phe Lys Asn Asp Ala Thr Cys Arg 210 215 220 Lys Phe Phe Thr Cys Leu Arg Pro Lys Gly Glu Pro Val Pro Asp Gln 225 230 235 240 Cys Pro Ile Gly Thr Val Phe Asn Gln Ala Thr Gln Ser Cys Asn Thr 245 250 255 Gly Thr Cys Glu Arg Lys Pro Lys Leu Tyr 260 265 32 802 DNA Ctenocephalides felis 32 ttaatataat ttaggtttcc tctcgcaagt tcctgtgttg cagctttgag tagcttggtt 60 aaatactgtt ccaatcggac attgatcagg aactggctct cctttaggac gtaaacacgt 120 gaagaacttc cggcatgttg cgtcgttctt gaaagcaaat tttccacgtg ctcggcattg 180 cccgacacag gtttcgctcc ttggggaaaa ctcggcgggt tcttcgcatt gcaatacggt 240 tcctcttccg gccatacata cgacgtacaa tgatttatca ccaatgtacg gaagcacctt 300 aggctgtgca ggacatgtaa ctgtatagca ttttgtgccc agagccatag gtccgcaatt 360 atgacgtaac ggatcatatg caaaatttgc ggggcaataa tattgggttc ctatactatt 420 ttcgtcacaa taataatagc tttgacaatc atttatattt ggataaaatc tcgatggtga 480 cggacatttg aaaccttgta ctttgcagac tgtactttct ttagtggtgc actgtagata 540 tccactttga tcagcgcaca cttgatctgg gctacaggat tccttaaccg gatctgctcc 600 tatttccgga caatactgca tttcgggtga actgcacgaa ttcacgcaac taaattttac 660 ttttttacat ttttgctcgg gtttcggtac aggtgtaggt tcaggagtag gtactactcc 720 tggtgcgggc tcatccaatg atgcaaatac ttctttattt actgttaaag gcgttccgtc 780 gacattaaac tcaccatcat aa 802 33 436 DNA Ctenocephalides felis misc_feature (347)..(347) n = unknown 33 cttgtatata agtgagtttt accaaagatg tgtgggccct acatattcgg aatgtttaat 60 tacacctctg cctcctccaa ttgttccgac tactacgact acgacaacta caccagcacc 120 gccgcctctt ttttcatgcg tgcaggaagg gatgtttata gatccctatg acagcacttg 180 caaaggatac tacaaatgtg ctttgaaagc aggcggagga ttcagtgtag cgcgttacaa 240 ttgtcctggt tctacttact tcagcagcac gtatcagcaa tgtgtggtcg cttccctttc 300 ggagtgccta agaaatcctc cagcgccttg gcaaccacct acgccgncgc catatacaac 360 tgcgtgcaga gcggtcattt gcagatccag cagacaaaac atgcaaagta tattttgaat 420 gtgtgagact ctctgg 436 34 485 DNA Ctenocephalides felis 34 ctcatttgca gatccagcag acaaaacatg caaagtatat tttgaatgtg tgagactctc 60 tggaggaaat ttcaacgtgg gtcgttttac atgcgccgga cagacatact tcagcgcgct 120 ttaccaacaa tgcgtgcaag cgcctttatc cgagtgcttg gcgacagctg cacctccacc 180 accgccacca ggaccaggac catcgcaacc ctttgcttgc gtgcggacag gattgttttt 240 ggattacact gataattctt gcaaatggta ctacgagtgc acattaaacg ctcaaggtgt 300 attcgacgtg gcccgatatg cttgtgcagc tgagttatat ttcaacagcg ttctgcagca 360 gtgcgtacct gcataccagt cagactgttt aggtgcaagt gtcacttctt cccatcatac 420 catccttacc atcttaccat ctttccaacc ttgcaacatc ttcccaacag caggattctt 480 ttggc 485 35 550 DNA Ctenocephalides felis 35 gtcgttttac atgcgccgga cagacatact tcagcgcgct ttaccaacaa tgcgtgcaag 60 cgcctttatc cgagtgcttg gcgacagctg cacctccacc accgccacca ggaccaggac 120 catcgcaacc ctttgcttgc gtgcggacag gattgttttt ggattacact gataattctt 180 gcaaatggta ctacgagtgc acattaaacg ctcaaggtgt attcgacgtg gcccgatatg 240 cttgtgcagc tgagttatat ttcaacagcg ttctgcagca gtgcgtacct gcataccagt 300 cagactgttt aggtgcaagt gtcacttctt ccccatccat accatcctta ccatccttac 360 catctttccc aaccttgcca acatcttccc caacagcagg atttcctttt ggccgaaaat 420 ctcttgatat gcaaacaaaa actggaacta aatgtacaaa aggagaagtt tctaaagacg 480 attaaattct acatgtccta gtgagtgtgt atatgaaatg ctattgatat acatcaagct 540 tataaggtat 550 36 436 DNA Ctenocephalides felis 36 taccatcttt cccaaccttg ccaacatctt ccccaacagc aggatttcct tttgaccgaa 60 aatctcttga tatgcaaaca aaaactggaa ctaaatgtac aaaaggagaa gtttctaaag 120 acgatttaaa ttctacatgt acctagtgag tgtgttatat gaaattgcta ttgcatatta 180 catacaagct taataaaggt tatggtgatt tatttcattt aaaggcaatg tatcggttta 240 atggttttaa atttattttt tatttaatat taaatagatt aattaaaaat ctataaagtg 300 ataagaggcc tgatatccat aatattattg aatattaaga agtgacggat agatctgata 360 atgtagtagt tggtatagac aatttactta ataattatga gtggcatcat tatgcaatga 420 tttatcacta ttattt 436 37 498 DNA Ctenocephalides felis misc_feature (23)..(23) n = unknown 37 aaaaggagaa gtttctaaag acnatttttn ttntacatgt acctagtgag tgtgnggggg 60 ggaaattgct attgcatatt acatacaagc ttaataaagg ttatggtgat ttatttcatt 120 taaaggcaan gtatcggntt aatggcnnta aatntatntt ttatttaata ttaaatagat 180 taattaaaaa tctataaagt gataagaggc ctgatatcca taatattatt gaatattaag 240 aagtgacgga tagatctgat aatgtagtag ttgttataga caatttactt aataattatg 300 agttgcatca ttatgcaatg atttatcact attatttatt caacatttta tttaactgct 360 tgcaactttt aataaaacgc atnttttatt gttttaagta taaatcttat tagggcacaa 420 tatgaaaata aaaataaaga actttataca aaagctnttt tatcaatatg cttcttgcgc 480 tattaagtta agaaattt 498 38 436 DNA Ctenocephalides felis misc_feature (64)..(64) n = unknown 38 aataaaattt cttaacttaa tagcgcaaga agcatattgt ataaaaaaag cttttgttaa 60 aggncttatt tttattttca tattgngccc taataagatt tatacttaaa acaataaaaa 120 atgcgtttta ttaaaagttg caagcagtta aataaaatgt tgaataaata atagngataa 180 atcattgcat aatgatgcaa ctcataatta ttaagtaaat tggctataac aactactaca 240 ttatcagatc tatccgtcac ttcttaatat tcaataatat tatggatatc aggcctctta 300 tcactttata gatttttaat taatctattt aatattaaat aaaaaataaa ttttaaaccn 360 tttaaccgat ccattgcctt taaangaaan aaataccata acctttatta agcttgttgt 420 aatatgcant agcaat 436 39 1513 DNA Ctenocephalides felis CDS (1)..(1017) 39 tac aag tgc aaa ctt ggt cta aat ggt gct ctg caa agc ggt cat ttt 48 Tyr Lys Cys Lys Leu Gly Leu Asn Gly Ala Leu Gln Ser Gly His Phe 1 5 10 15 tta tgc cca tgg aac ttg tat ata agt gag ttt tac caa aga tgt gtg 96 Leu Cys Pro Trp Asn Leu Tyr Ile Ser Glu Phe Tyr Gln Arg Cys Val 20 25 30 ggc cct aca tat tcg gaa tgt tta att aca cct ctg cct cct cca att 144 Gly Pro Thr Tyr Ser Glu Cys Leu Ile Thr Pro Leu Pro Pro Pro Ile 35 40 45 gtt ccg act act acg act acg aca act aca cca gca ccg ccg cct ctt 192 Val Pro Thr Thr Thr Thr Thr Thr Thr Thr Pro Ala Pro Pro Pro Leu 50 55 60 ttt tca tgc gtg cag gaa ggg atg ttt ata gat ccc tat gac agc act 240 Phe Ser Cys Val Gln Glu Gly Met Phe Ile Asp Pro Tyr Asp Ser Thr 65 70 75 80 tgc aaa gga tac tac aaa tgt gct ttg aaa gca ggc gga gga ttc agt 288 Cys Lys Gly Tyr Tyr Lys Cys Ala Leu Lys Ala Gly Gly Gly Phe Ser 85 90 95 gta gcg cgt tac aat tgt cct ggt tct act tac ttc agc agc acg tat 336 Val Ala Arg Tyr Asn Cys Pro Gly Ser Thr Tyr Phe Ser Ser Thr Tyr 100 105 110 cag caa tgt gtg gtc gct tcc ctt tcg gag tgc cta aga aat cct cca 384 Gln Gln Cys Val Val Ala Ser Leu Ser Glu Cys Leu Arg Asn Pro Pro 115 120 125 gcg cct tgg caa cca cct acg ccg ccg ccc ata tac aac tgc gtg cag 432 Ala Pro Trp Gln Pro Pro Thr Pro Pro Pro Ile Tyr Asn Cys Val Gln 130 135 140 agc ggc tca ttt gca gat cca gca gac aaa aca tgc aaa gta tat ttt 480 Ser Gly Ser Phe Ala Asp Pro Ala Asp Lys Thr Cys Lys Val Tyr Phe 145 150 155 160 gaa tgt gtg aga ctc tct gga gga aat ttc aac gtg ggt cgt ttt aca 528 Glu Cys Val Arg Leu Ser Gly Gly Asn Phe Asn Val Gly Arg Phe Thr 165 170 175 tgc gcc gga cag aca tac ttc agc gcg ctt tac caa caa tgc gtg caa 576 Cys Ala Gly Gln Thr Tyr Phe Ser Ala Leu Tyr Gln Gln Cys Val Gln 180 185 190 gcg cct tta tcc gag tgc ttg gcg aca gct gca cct cca cca ccg cca 624 Ala Pro Leu Ser Glu Cys Leu Ala Thr Ala Ala Pro Pro Pro Pro Pro 195 200 205 cca gga cca gga cca tcg caa ccc ttt gct tgc gtg cgg aca gga ttg 672 Pro Gly Pro Gly Pro Ser Gln Pro Phe Ala Cys Val Arg Thr Gly Leu 210 215 220 ttt ttg gat tac act gat aat tct tgc aaa tgg tac tac gag tgc aca 720 Phe Leu Asp Tyr Thr Asp Asn Ser Cys Lys Trp Tyr Tyr Glu Cys Thr 225 230 235 240 tta aac gct caa ggt gta ttc gac gtg gcc cga tat gct tgt gca gct 768 Leu Asn Ala Gln Gly Val Phe Asp Val Ala Arg Tyr Ala Cys Ala Ala 245 250 255 gag tta tat ttc aac agc gtt ctg cag cag tgc gta cct gca tac cag 816 Glu Leu Tyr Phe Asn Ser Val Leu Gln Gln Cys Val Pro Ala Tyr Gln 260 265 270 tca gac tgt tta ggt gca agt gtc act tct tcc cca tcc ata cca tcc 864 Ser Asp Cys Leu Gly Ala Ser Val Thr Ser Ser Pro Ser Ile Pro Ser 275 280 285 tta cca tcc tta cca tct ttc cca acc ttg cca aca tct tcc cca aca 912 Leu Pro Ser Leu Pro Ser Phe Pro Thr Leu Pro Thr Ser Ser Pro Thr 290 295 300 gca gga ttt cct ttt ggc cga aaa tct ctt gat atg caa aca aaa act 960 Ala Gly Phe Pro Phe Gly Arg Lys Ser Leu Asp Met Gln Thr Lys Thr 305 310 315 320 gga act aaa tgt aca aaa gga gaa gtt tct aaa gac gat tta aat tct 1008 Gly Thr Lys Cys Thr Lys Gly Glu Val Ser Lys Asp Asp Leu Asn Ser 325 330 335 aca tgt acc tagtgagtgt gttatatgaa attgctattg catattacat 1057 Thr Cys Thr acaagcttaa taaaggttat ggtgatttat ttcatttaaa ggcaatgtat cggtttaatg 1117 gttttaaatt tattttttat ttaatattaa atagattaat taaaaatcta taaagtgata 1177 agaggcctga tatccataat attattgaat attaagaagt gacggataga tctgataatg 1237 tagtagttgg tatagacaat ttacttaata attatgagtg gcatcattat gcaatgattt 1297 atcactatta tttattcaac attttattta actgcttgca acttttaata aaacgcatnt 1357 tttattgttt taagtataaa tcttattagg gcacaatatg aaaataaaaa taaagaactt 1417 tatacaaaag ctnttttatc aatatgcttc ttgcgctatt aagttaagaa attttattta 1477 tgctgggaaa aaancaanaa atgctttatt tattct 1513 40 339 PRT Ctenocephalides felis 40 Tyr Lys Cys Lys Leu Gly Leu Asn Gly Ala Leu Gln Ser Gly His Phe 1 5 10 15 Leu Cys Pro Trp Asn Leu Tyr Ile Ser Glu Phe Tyr Gln Arg Cys Val 20 25 30 Gly Pro Thr Tyr Ser Glu Cys Leu Ile Thr Pro Leu Pro Pro Pro Ile 35 40 45 Val Pro Thr Thr Thr Thr Thr Thr Thr Thr Pro Ala Pro Pro Pro Leu 50 55 60 Phe Ser Cys Val Gln Glu Gly Met Phe Ile Asp Pro Tyr Asp Ser Thr 65 70 75 80 Cys Lys Gly Tyr Tyr Lys Cys Ala Leu Lys Ala Gly Gly Gly Phe Ser 85 90 95 Val Ala Arg Tyr Asn Cys Pro Gly Ser Thr Tyr Phe Ser Ser Thr Tyr 100 105 110 Gln Gln Cys Val Val Ala Ser Leu Ser Glu Cys Leu Arg Asn Pro Pro 115 120 125 Ala Pro Trp Gln Pro Pro Thr Pro Pro Pro Ile Tyr Asn Cys Val Gln 130 135 140 Ser Gly Ser Phe Ala Asp Pro Ala Asp Lys Thr Cys Lys Val Tyr Phe 145 150 155 160 Glu Cys Val Arg Leu Ser Gly Gly Asn Phe Asn Val Gly Arg Phe Thr 165 170 175 Cys Ala Gly Gln Thr Tyr Phe Ser Ala Leu Tyr Gln Gln Cys Val Gln 180 185 190 Ala Pro Leu Ser Glu Cys Leu Ala Thr Ala Ala Pro Pro Pro Pro Pro 195 200 205 Pro Gly Pro Gly Pro Ser Gln Pro Phe Ala Cys Val Arg Thr Gly Leu 210 215 220 Phe Leu Asp Tyr Thr Asp Asn Ser Cys Lys Trp Tyr Tyr Glu Cys Thr 225 230 235 240 Leu Asn Ala Gln Gly Val Phe Asp Val Ala Arg Tyr Ala Cys Ala Ala 245 250 255 Glu Leu Tyr Phe Asn Ser Val Leu Gln Gln Cys Val Pro Ala Tyr Gln 260 265 270 Ser Asp Cys Leu Gly Ala Ser Val Thr Ser Ser Pro Ser Ile Pro Ser 275 280 285 Leu Pro Ser Leu Pro Ser Phe Pro Thr Leu Pro Thr Ser Ser Pro Thr 290 295 300 Ala Gly Phe Pro Phe Gly Arg Lys Ser Leu Asp Met Gln Thr Lys Thr 305 310 315 320 Gly Thr Lys Cys Thr Lys Gly Glu Val Ser Lys Asp Asp Leu Asn Ser 325 330 335 Thr Cys Thr 41 1513 DNA Ctenocephalides felis misc_feature (19)..(19) n = unknown 41 agaataaata aagcatttnt tgnttttttc ccagcataaa taaaatttct taacttaata 60 gcgcaagaag catattgata aaanagcttt tgtataaagt tctttatttt tattttcata 120 ttgtgcccta ataagattta tacttaaaac aataaaanat gcgttttatt aaaagttgca 180 agcagttaaa taaaatgttg aataaataat agtgataaat cattgcataa tgatgccact 240 cataattatt aagtaaattg tctataccaa ctactacatt atcagatcta tccgtcactt 300 cttaatattc aataatatta tggatatcag gcctcttatc actttataga tttttaatta 360 atctatttaa tattaaataa aaaataaatt taaaaccatt aaaccgatac attgccttta 420 aatgaaataa atcaccataa cctttattaa gcttgtatgt aatatgcaat agcaatttca 480 tataacacac tcactaggta catgtagaat ttaaatcgtc tttagaaact tctccttttg 540 tacatttagt tccagttttt gtttgcatat caagagattt tcggccaaaa ggaaatcctg 600 ctgttgggga agatgttggc aaggttggga aagatggtaa ggatggtaag gatggtatgg 660 atggggaaga agtgacactt gcacctaaac agtctgactg gtatgcaggt acgcactgct 720 gcagaacgct gttgaaatat aactcagctg cacaagcata tcgggccacg tcgaatacac 780 cttgagcgtt taatgtgcac tcgtagtacc atttgcaaga attatcagtg taatccaaaa 840 acaatcctgt ccgcacgcaa gcaaagggtt gcgatggtcc tggtcctggt ggcggtggtg 900 gaggtgcagc tgtcgccaag cactcggata aaggcgcttg cacgcattgt tggtaaagcg 960 cgctgaagta tgtctgtccg gcgcatgtaa aacgacccac gttgaaattt cctccagaga 1020 gtctcacaca ttcaaaatat actttgcatg ttttgtctgc tggatctgca aatgagccgc 1080 tctgcacgca gttgtatatg ggcggcggcg taggtggttg ccaaggcgct ggaggatttc 1140 ttaggcactc cgaaagggaa gcgaccacac attgctgata cgtgctgctg aagtaagtag 1200 aaccaggaca attgtaacgc gctacactga atcctccgcc tgctttcaaa gcacatttgt 1260 agtatccttt gcaagtgctg tcatagggat ctataaacat cccttcctgc acgcatgaaa 1320 aaagaggcgg cggtgctggt gtagttgtcg tagtcgtagt agtcggaaca attggaggag 1380 gcagaggtgt aattaaacat tccgaatatg tagggcccac acatctttgg taaaactcac 1440 ttatatacaa gttccatggg cataaaaaat gaccgctttg cagagcacca tttagaccaa 1500 gtttgcactt gta 1513 42 1832 DNA Ctenocephalides felis CDS (146)..(1336) 42 tcnaatacga ctcactatag ggcaagcagt gggtaacaac gcagagtacg cgggggattt 60 agtaacttca aatttttggt acaatatcat tatcaaaaaa attgtttatt ttaaagtgga 120 atctgttaaa tcgcgtttca ttacg atg aaa ctg ctt tta gtg ctc ttg gct 172 Met Lys Leu Leu Leu Val Leu Leu Ala 1 5 acc ttc tcg tgt aca ttg tct ttg gag tct caa gaa cta att cca gtt 220 Thr Phe Ser Cys Thr Leu Ser Leu Glu Ser Gln Glu Leu Ile Pro Val 10 15 20 25 gaa aca aat tcc agc agc gtt cgt att tgg cag caa cca ttc att tgt 268 Glu Thr Asn Ser Ser Ser Val Arg Ile Trp Gln Gln Pro Phe Ile Cys 30 35 40 cca aga gtt gga cta ttt cgc gat cca ttg gat cct aca tgc aaa agg 316 Pro Arg Val Gly Leu Phe Arg Asp Pro Leu Asp Pro Thr Cys Lys Arg 45 50 55 tac tac aag tgc aaa ctt ggt cta aat ggt gct ctg caa agc ggt cat 364 Tyr Tyr Lys Cys Lys Leu Gly Leu Asn Gly Ala Leu Gln Ser Gly His 60 65 70 ttt tta tgc cca tgg aac ttg tat ata agt gag ttt tac caa aga tgt 412 Phe Leu Cys Pro Trp Asn Leu Tyr Ile Ser Glu Phe Tyr Gln Arg Cys 75 80 85 gtg ggc cct aca tat tcg gaa tgt tta att aca cct ctg cct cct cca 460 Val Gly Pro Thr Tyr Ser Glu Cys Leu Ile Thr Pro Leu Pro Pro Pro 90 95 100 105 att gtt ccg act act acg act acg aca act aca cca gca ccg ccg cct 508 Ile Val Pro Thr Thr Thr Thr Thr Thr Thr Thr Pro Ala Pro Pro Pro 110 115 120 ctt ttt tca tgc gtg cag gaa ggg atg ttt ata gat ccc tat gac agc 556 Leu Phe Ser Cys Val Gln Glu Gly Met Phe Ile Asp Pro Tyr Asp Ser 125 130 135 act tgc aaa gga tac tac aaa tgt gct ttg aaa gca ggc gga gga ttc 604 Thr Cys Lys Gly Tyr Tyr Lys Cys Ala Leu Lys Ala Gly Gly Gly Phe 140 145 150 agt gta gcg cgt tac aat tgt cct ggt tct act tac ttc agc agc acg 652 Ser Val Ala Arg Tyr Asn Cys Pro Gly Ser Thr Tyr Phe Ser Ser Thr 155 160 165 tat cag caa tgt gtg gtc gct tcc ctt tcg gag tgc cta aga aat cct 700 Tyr Gln Gln Cys Val Val Ala Ser Leu Ser Glu Cys Leu Arg Asn Pro 170 175 180 185 cca gcg cct tgg caa cca cct acg ccg ccg ccc ata tac aac tgc gtg 748 Pro Ala Pro Trp Gln Pro Pro Thr Pro Pro Pro Ile Tyr Asn Cys Val 190 195 200 cag agc ggc tca ttt gca gat cca gca gac aaa aca tgc aaa gta tat 796 Gln Ser Gly Ser Phe Ala Asp Pro Ala Asp Lys Thr Cys Lys Val Tyr 205 210 215 ttt gaa tgt gtg aga ctc tct gga gga aat ttc aac gtg ggt cgt ttt 844 Phe Glu Cys Val Arg Leu Ser Gly Gly Asn Phe Asn Val Gly Arg Phe 220 225 230 aca tgc gcc gga cag aca tac ttc agc gcg ctt tac caa caa tgc gtg 892 Thr Cys Ala Gly Gln Thr Tyr Phe Ser Ala Leu Tyr Gln Gln Cys Val 235 240 245 caa gcg cct tta tcc gag tgc ttg gcg aca gct gca cct cca cca ccg 940 Gln Ala Pro Leu Ser Glu Cys Leu Ala Thr Ala Ala Pro Pro Pro Pro 250 255 260 265 cca cca gga cca gga cca tcg caa ccc ttt gct tgc gtg cgg aca gga 988 Pro Pro Gly Pro Gly Pro Ser Gln Pro Phe Ala Cys Val Arg Thr Gly 270 275 280 ttg ttt ttg gat tac act gat aat tct tgc aaa tgg tac tac gag tgc 1036 Leu Phe Leu Asp Tyr Thr Asp Asn Ser Cys Lys Trp Tyr Tyr Glu Cys 285 290 295 aca tta aac gct caa ggt gta ttc gac gtg gcc cga tat gct tgt gca 1084 Thr Leu Asn Ala Gln Gly Val Phe Asp Val Ala Arg Tyr Ala Cys Ala 300 305 310 gct gag tta tat ttc aac agc gtt ctg cag cag tgc gta cct gca tac 1132 Ala Glu Leu Tyr Phe Asn Ser Val Leu Gln Gln Cys Val Pro Ala Tyr 315 320 325 cag tca gac tgt tta ggt gca agt gtc act tct tcc cca tcc ata cca 1180 Gln Ser Asp Cys Leu Gly Ala Ser Val Thr Ser Ser Pro Ser Ile Pro 330 335 340 345 tcc tta cca tcc tta cca tct ttc cca acc ttg cca aca tct tcc cca 1228 Ser Leu Pro Ser Leu Pro Ser Phe Pro Thr Leu Pro Thr Ser Ser Pro 350 355 360 aca gca gga ttt cct ttt ggc cga aaa tct ctt gat atg caa aca aaa 1276 Thr Ala Gly Phe Pro Phe Gly Arg Lys Ser Leu Asp Met Gln Thr Lys 365 370 375 act gga act aaa tgt aca aaa gga gaa gtt tct aaa gac gat tta aat 1324 Thr Gly Thr Lys Cys Thr Lys Gly Glu Val Ser Lys Asp Asp Leu Asn 380 385 390 tct aca tgt acc tagtgagtgt gttatatgaa attgctattg catattacat 1376 Ser Thr Cys Thr 395 acaagcttaa taaaggttat ggtgatttat ttcatttaaa ggcaatgtat cggtttaatg 1436 gttttaaatt tattttttat ttaatattaa atagattaat taaaaatcta taaagtgata 1496 agaggcctga tatccataat attattgaat attaagaagt gacggataga tctgataatg 1556 tagtagttgg tatagacaat ttacttaata attatgagtg gcatcattat gcaatgattt 1616 atcactatta tttattcaac attttattta actgcttgca acttttaata aaacgcatnt 1676 tttattgttt taagtataaa tcttattagg gcacaatatg aaaataaaaa taaagaactt 1736 tatacaaaag ctnttttatc aatatgcttc ttgcgctatt aagttaagaa attttattta 1796 tgctgggaaa aaancaanaa atgctttatt tattct 1832 43 397 PRT Ctenocephalides felis 43 Met Lys Leu Leu Leu Val Leu Leu Ala Thr Phe Ser Cys Thr Leu Ser 1 5 10 15 Leu Glu Ser Gln Glu Leu Ile Pro Val Glu Thr Asn Ser Ser Ser Val 20 25 30 Arg Ile Trp Gln Gln Pro Phe Ile Cys Pro Arg Val Gly Leu Phe Arg 35 40 45 Asp Pro Leu Asp Pro Thr Cys Lys Arg Tyr Tyr Lys Cys Lys Leu Gly 50 55 60 Leu Asn Gly Ala Leu Gln Ser Gly His Phe Leu Cys Pro Trp Asn Leu 65 70 75 80 Tyr Ile Ser Glu Phe Tyr Gln Arg Cys Val Gly Pro Thr Tyr Ser Glu 85 90 95 Cys Leu Ile Thr Pro Leu Pro Pro Pro Ile Val Pro Thr Thr Thr Thr 100 105 110 Thr Thr Thr Thr Pro Ala Pro Pro Pro Leu Phe Ser Cys Val Gln Glu 115 120 125 Gly Met Phe Ile Asp Pro Tyr Asp Ser Thr Cys Lys Gly Tyr Tyr Lys 130 135 140 Cys Ala Leu Lys Ala Gly Gly Gly Phe Ser Val Ala Arg Tyr Asn Cys 145 150 155 160 Pro Gly Ser Thr Tyr Phe Ser Ser Thr Tyr Gln Gln Cys Val Val Ala 165 170 175 Ser Leu Ser Glu Cys Leu Arg Asn Pro Pro Ala Pro Trp Gln Pro Pro 180 185 190 Thr Pro Pro Pro Ile Tyr Asn Cys Val Gln Ser Gly Ser Phe Ala Asp 195 200 205 Pro Ala Asp Lys Thr Cys Lys Val Tyr Phe Glu Cys Val Arg Leu Ser 210 215 220 Gly Gly Asn Phe Asn Val Gly Arg Phe Thr Cys Ala Gly Gln Thr Tyr 225 230 235 240 Phe Ser Ala Leu Tyr Gln Gln Cys Val Gln Ala Pro Leu Ser Glu Cys 245 250 255 Leu Ala Thr Ala Ala Pro Pro Pro Pro Pro Pro Gly Pro Gly Pro Ser 260 265 270 Gln Pro Phe Ala Cys Val Arg Thr Gly Leu Phe Leu Asp Tyr Thr Asp 275 280 285 Asn Ser Cys Lys Trp Tyr Tyr Glu Cys Thr Leu Asn Ala Gln Gly Val 290 295 300 Phe Asp Val Ala Arg Tyr Ala Cys Ala Ala Glu Leu Tyr Phe Asn Ser 305 310 315 320 Val Leu Gln Gln Cys Val Pro Ala Tyr Gln Ser Asp Cys Leu Gly Ala 325 330 335 Ser Val Thr Ser Ser Pro Ser Ile Pro Ser Leu Pro Ser Leu Pro Ser 340 345 350 Phe Pro Thr Leu Pro Thr Ser Ser Pro Thr Ala Gly Phe Pro Phe Gly 355 360 365 Arg Lys Ser Leu Asp Met Gln Thr Lys Thr Gly Thr Lys Cys Thr Lys 370 375 380 Gly Glu Val Ser Lys Asp Asp Leu Asn Ser Thr Cys Thr 385 390 395 44 1832 DNA Ctenocephalides felis misc_feature (19)..(19) n = unknown 44 agaataaata aagcatttnt tgnttttttc ccagcataaa taaaatttct taacttaata 60 gcgcaagaag catattgata aaanagcttt tgtataaagt tctttatttt tattttcata 120 ttgtgcccta ataagattta tacttaaaac aataaaanat gcgttttatt aaaagttgca 180 45 1191 DNA Ctenocephalides felis 45 atgaaactgc ttttagtgct cttggctacc ttctcgtgta cattgtcttt ggagtctcaa 60 gaactaattc cagttgaaac aaattccagc agcgttcgta tttggcagca accattcatt 120 tgtccaagag ttggactatt tcgcgatcca ttggatccta catgcaaaag gtactacaag 180 tgcaaacttg gtctaaatgg tgctctgcaa agcggtcatt ttttatgccc atggaacttg 240 46 1191 DNA Ctenocephalides felis 46 ggtacatgta gaatttaaat cgtctttaga aacttctcct tttgtacatt tagttccagt 60 ttttgtttgc atatcaagag attttcggcc aaaaggaaat cctgctgttg gggaagatgt 120 tggcaaggtt gggaaagatg gtaaggatgg taaggatggt atggatgggg aagaagtgac 180 acttgcacct aaacagtctg actggtatgc aggtacgcac tgctgcagaa cgctgttgaa 240 atataactca gctgcacaag catatcgggc cacgtcgaat acaccttgag cgtttaatgt 300 gcactcgtag taccatttgc aagaattatc agtgtaatcc aaaaacaatc ctgtccgcac 360 gcaagcaaag ggttgcgatg gtcctggtcc tggtggcggt ggtggaggtg cagctgtcgc 420 caagcactcg gataaaggcg cttgcacgca ttgttggtaa agcgcgctga agtatgtctg 480 tccggcgcat gtaaaacgac ccacgttgaa atttcctcca gagagtctca cacattcaaa 540 atatactttg catgttttgt ctgctggatc tgcaaatgag ccgctctgca cgcagttgta 600 tatgggcggc ggcgtaggtg gttgccaagg cgctggagga tttcttaggc actccgaaag 660 ggaagcgacc acacattgct gatacgtgct gctgaagtaa gtagaaccag gacaattgta 720 acgcgctaca ctgaatcctc cgcctgcttt caaagcacat ttgtagtatc ctttgcaagt 780 gctgtcatag ggatctataa acatcccttc ctgcacgcat gaaaaaagag gcggcggtgc 840 tggtgtagtt gtcgtagtcg tagtagtcgg aacaattgga ggaggcagag gtgtaattaa 900 acattccgaa tatgtagggc ccacacatct ttggtaaaac tcacttatat acaagttcca 960 tgggcataaa aaatgaccgc tttgcagagc accatttaga ccaagtttgc acttgtagta 1020 ccttttgcat gtaggatcca atggatcgcg aaatagtcca actcttggac aaatgaatgg 1080 ttgctgccaa atacgaacgc tgctggaatt tgtttcaact ggaattagtt cttgagactc 1140 caaagacaat gtacacgaga aggtagccaa gagcactaaa agcagtttca t 1191 47 1161 DNA Ctenocephalides felis CDS (1)..(1143) 47 ttg gag tct caa gaa cta att cca gtt gaa aca aat tcc agc agc gtt 48 Leu Glu Ser Gln Glu Leu Ile Pro Val Glu Thr Asn Ser Ser Ser Val 1 5 10 15 cgt att tgg cag caa cca ttc att tgt cca aga gtt gga cta ttt cgc 96 Arg Ile Trp Gln Gln Pro Phe Ile Cys Pro Arg Val Gly Leu Phe Arg 20 25 30 gat cca ttg gat cct aca tgc aaa agg tac tac aag tgc aaa ctt ggt 144 Asp Pro Leu Asp Pro Thr Cys Lys Arg Tyr Tyr Lys Cys Lys Leu Gly 35 40 45 cta aat ggt gct ctg caa agc ggt cat ttt tta tgc cca tgg aac ttg 192 Leu Asn Gly Ala Leu Gln Ser Gly His Phe Leu Cys Pro Trp Asn Leu 50 55 60 tat ata agt gag ttt tac caa aga tgt gtg ggc cct aca tat tcg gaa 240 Tyr Ile Ser Glu Phe Tyr Gln Arg Cys Val Gly Pro Thr Tyr Ser Glu 65 70 75 80 tgt tta att aca cct ctg cct cct cca att gtt ccg act act acg act 288 Cys Leu Ile Thr Pro Leu Pro Pro Pro Ile Val Pro Thr Thr Thr Thr 85 90 95 acg aca act aca cca gca ccg ccg cct ctt ttt tca tgc gtg cag gaa 336 Thr Thr Thr Thr Pro Ala Pro Pro Pro Leu Phe Ser Cys Val Gln Glu 100 105 110 ggg atg ttt ata gat ccc tat gac agc act tgc aaa gga tac tac aaa 384 Gly Met Phe Ile Asp Pro Tyr Asp Ser Thr Cys Lys Gly Tyr Tyr Lys 115 120 125 tgt gct ttg aaa gca ggc gga gga ttc agt gta gcg cgt tac aat tgt 432 Cys Ala Leu Lys Ala Gly Gly Gly Phe Ser Val Ala Arg Tyr Asn Cys 130 135 140 cct ggt tct act tac ttc agc agc acg tat cag caa tgt gtg gtc gct 480 Pro Gly Ser Thr Tyr Phe Ser Ser Thr Tyr Gln Gln Cys Val Val Ala 145 150 155 160 tcc ctt tcg gag tgc cta aga aat cct cca gcg cct tgg caa cca cct 528 Ser Leu Ser Glu Cys Leu Arg Asn Pro Pro Ala Pro Trp Gln Pro Pro 165 170 175 acg ccg ccg ccc ata tac aac tgc gtg cag agc ggc tca ttt gca gat 576 Thr Pro Pro Pro Ile Tyr Asn Cys Val Gln Ser Gly Ser Phe Ala Asp 180 185 190 cca gca gac aaa aca tgc aaa gta tat ttt gaa tgt gtg aga ctc tct 624 Pro Ala Asp Lys Thr Cys Lys Val Tyr Phe Glu Cys Val Arg Leu Ser 195 200 205 gga gga aat ttc aac gtg ggt cgt ttt aca tgc gcc gga cag aca tac 672 Gly Gly Asn Phe Asn Val Gly Arg Phe Thr Cys Ala Gly Gln Thr Tyr 210 215 220 ttc agc gcg ctt tac caa caa tgc gtg caa gcg cct tta tcc gag tgc 720 Phe Ser Ala Leu Tyr Gln Gln Cys Val Gln Ala Pro Leu Ser Glu Cys 225 230 235 240 ttg gcg aca gct gca cct cca cca ccg cca cca gga cca gga cca tcg 768 Leu Ala Thr Ala Ala Pro Pro Pro Pro Pro Pro Gly Pro Gly Pro Ser 245 250 255 caa ccc ttt gct tgc gtg cgg aca gga ttg ttt ttg gat tac act gat 816 Gln Pro Phe Ala Cys Val Arg Thr Gly Leu Phe Leu Asp Tyr Thr Asp 260 265 270 aat tct tgc aaa tgg tac tac gag tgc aca tta aac gct caa ggt gta 864 Asn Ser Cys Lys Trp Tyr Tyr Glu Cys Thr Leu Asn Ala Gln Gly Val 275 280 285 ttc gac gtg gcc cga tat gct tgt gca gct gag tta tat ttc aac agc 912 Phe Asp Val Ala Arg Tyr Ala Cys Ala Ala Glu Leu Tyr Phe Asn Ser 290 295 300 gtt ctg cag cag tgc gta cct gca tac cag tca gac tgt tta ggt gca 960 Val Leu Gln Gln Cys Val Pro Ala Tyr Gln Ser Asp Cys Leu Gly Ala 305 310 315 320 agt gtc act tct tcc cca tcc ata cca tcc tta cca tcc tta cca tct 1008 Ser Val Thr Ser Ser Pro Ser Ile Pro Ser Leu Pro Ser Leu Pro Ser 325 330 335 ttc cca acc ttg cca aca tct tcc cca aca gca gga ttt cct ttt ggc 1056 Phe Pro Thr Leu Pro Thr Ser Ser Pro Thr Ala Gly Phe Pro Phe Gly 340 345 350 cga aaa tct ctt gat atg caa aca aaa act gga act aaa tgt aca aaa 1104 Arg Lys Ser Leu Asp Met Gln Thr Lys Thr Gly Thr Lys Cys Thr Lys 355 360 365 gga gaa gtt tct aaa gac gat tta aat tct aca tgt acc tagtgagtgt 1153 Gly Glu Val Ser Lys Asp Asp Leu Asn Ser Thr Cys Thr 370 375 380 gttatatg 1161 48 381 PRT Ctenocephalides felis 48 Leu Glu Ser Gln Glu Leu Ile Pro Val Glu Thr Asn Ser Ser Ser Val 1 5 10 15 Arg Ile Trp Gln Gln Pro Phe Ile Cys Pro Arg Val Gly Leu Phe Arg 20 25 30 Asp Pro Leu Asp Pro Thr Cys Lys Arg Tyr Tyr Lys Cys Lys Leu Gly 35 40 45 Leu Asn Gly Ala Leu Gln Ser Gly His Phe Leu Cys Pro Trp Asn Leu 50 55 60 Tyr Ile Ser Glu Phe Tyr Gln Arg Cys Val Gly Pro Thr Tyr Ser Glu 65 70 75 80 Cys Leu Ile Thr Pro Leu Pro Pro Pro Ile Val Pro Thr Thr Thr Thr 85 90 95 Thr Thr Thr Thr Pro Ala Pro Pro Pro Leu Phe Ser Cys Val Gln Glu 100 105 110 Gly Met Phe Ile Asp Pro Tyr Asp Ser Thr Cys Lys Gly Tyr Tyr Lys 115 120 125 Cys Ala Leu Lys Ala Gly Gly Gly Phe Ser Val Ala Arg Tyr Asn Cys 130 135 140 Pro Gly Ser Thr Tyr Phe Ser Ser Thr Tyr Gln Gln Cys Val Val Ala 145 150 155 160 Ser Leu Ser Glu Cys Leu Arg Asn Pro Pro Ala Pro Trp Gln Pro Pro 165 170 175 Thr Pro Pro Pro Ile Tyr Asn Cys Val Gln Ser Gly Ser Phe Ala Asp 180 185 190 Pro Ala Asp Lys Thr Cys Lys Val Tyr Phe Glu Cys Val Arg Leu Ser 195 200 205 Gly Gly Asn Phe Asn Val Gly Arg Phe Thr Cys Ala Gly Gln Thr Tyr 210 215 220 Phe Ser Ala Leu Tyr Gln Gln Cys Val Gln Ala Pro Leu Ser Glu Cys 225 230 235 240 Leu Ala Thr Ala Ala Pro Pro Pro Pro Pro Pro Gly Pro Gly Pro Ser 245 250 255 Gln Pro Phe Ala Cys Val Arg Thr Gly Leu Phe Leu Asp Tyr Thr Asp 260 265 270 Asn Ser Cys Lys Trp Tyr Tyr Glu Cys Thr Leu Asn Ala Gln Gly Val 275 280 285 Phe Asp Val Ala Arg Tyr Ala Cys Ala Ala Glu Leu Tyr Phe Asn Ser 290 295 300 Val Leu Gln Gln Cys Val Pro Ala Tyr Gln Ser Asp Cys Leu Gly Ala 305 310 315 320 Ser Val Thr Ser Ser Pro Ser Ile Pro Ser Leu Pro Ser Leu Pro Ser 325 330 335 Phe Pro Thr Leu Pro Thr Ser Ser Pro Thr Ala Gly Phe Pro Phe Gly 340 345 350 Arg Lys Ser Leu Asp Met Gln Thr Lys Thr Gly Thr Lys Cys Thr Lys 355 360 365 Gly Glu Val Ser Lys Asp Asp Leu Asn Ser Thr Cys Thr 370 375 380 49 1161 DNA Ctenocephalides felis 49 catataacac actcactagg tacatgtaga atttaaatcg tctttagaaa cttctccttt 60 tgtacattta gttccagttt ttgtttgcat atcaagagat tttcggccaa aaggaaatcc 120 tgctgttggg gaagatgttg gcaaggttgg gaaagatggt aaggatggta aggatggtat 180 ggatggggaa gaagtgacac ttgcacctaa acagtctgac tggtatgcag gtacgcactg 240 ctgcagaacg ctgttgaaat ataactcagc tgcacaagca tatcgggcca cgtcgaatac 300 accttgagcg tttaatgtgc actcgtagta ccatttgcaa gaattatcag tgtaatccaa 360 aaacaatcct gtccgcacgc aagcaaaggg ttgcgatggt cctggtcctg gtggcggtgg 420 tggaggtgca gctgtcgcca agcactcgga taaaggcgct tgcacgcatt gttggtaaag 480 cgcgctgaag tatgtctgtc cggcgcatgt aaaacgaccc acgttgaaat ttcctccaga 540 gagtctcaca cattcaaaat atactttgca tgttttgtct gctggatctg caaatgagcc 600 gctctgcacg cagttgtata tgggcggcgg cgtaggtggt tgccaaggcg ctggaggatt 660 tcttaggcac tccgaaaggg aagcgaccac acattgctga tacgtgctgc tgaagtaagt 720 agaaccagga caattgtaac gcgctacact gaatcctccg cctgctttca aagcacattt 780 gtagtatcct ttgcaagtgc tgtcataggg atctataaac atcccttcct gcacgcatga 840 aaaaagaggc ggcggtgctg gtgtagttgt cgtagtcgta gtagtcggaa caattggagg 900 aggcagaggt gtaattaaac attccgaata tgtagggccc acacatcttt ggtaaaactc 960 acttatatac aagttccatg ggcataaaaa atgaccgctt tgcagagcac catttagacc 1020 aagtttgcac ttgtagtacc ttttgcatgt aggatccaat ggatcgcgaa atagtccaac 1080 tcttggacaa atgaatggtt gctgccaaat acgaacgctg ctggaatttg tttcaactgg 1140 aattagttct tgagactcca a 1161 50 29 DNA Artificial sequence Synthetic Primer 50 cgggatcctg ctgacaggaa ttcgcccac 29 51 31 DNA Artificial sequence Synthetic Primer 51 catggtaccc ctggtttaag ccttacttag c 31 52 21 DNA Artificial sequence Synthetic Primer 52 gtctggaagc tcaggaagag g 21 53 27 DNA Artificial sequence Synthetic Primer 53 ccatcctaat acgactcact atagggc 27 54 23 DNA Artificial sequence Synthetic Primer 54 actcactata gggctcgagc ggc 23 55 24 DNA Artificial sequence Synthetic Primer 55 gtaatatgcg tgacaatcgt gtgg 24 56 24 DNA Artificial sequence Synthetic Primer 56 cggtgcaagt tatagaacct tccg 24 57 32 DNA Artificial sequence Synthetic Primer 57 cgggatcccg aatatgctga cgtagatgtg tg 32 58 32 DNA Artificial sequence Synthetic Primer 58 ggaattctgt tttattctgg ttggtaacat tc 32 59 23 DNA Artificial sequence Synthetic Primer 59 gatatccact ttgatcagcg cac 23 60 21 DNA Artificial sequence Synthetic Primer 60 ggtactactc ctggtgcggg c 21 61 23 DNA Artificial sequence Synthetic Primer 61 ccgtcgacat taaactcacc atc 23 62 31 DNA Artificial sequence Synthetic Primer 62 cgggatcctt atgatggtga gtttaatgtc g 31 63 34 DNA Artificial sequence Synthetic Primer 63 ggggtacctt aatataattt aggtttcctc tcgc 34 64 21 DNA Artificial sequence Synthetic Primer 64 gcgcatgtaa aacgacccac g 21 65 45 DNA Artificial sequence Synthetic Primer 65 ctaatacgac tcactatagg gcaagcagtg gtaacaacgc agagt 45 66 22 DNA Artificial sequence Synthetic Primer 66 ctaatacgac tcactatagg gc 22 67 30 DNA Artificial sequence Synthetic Primer 67 cgggtacctt ggagtctcaa gaactaattc 30 68 37 DNA Artificial sequence Synthetic Primer 68 aggaattcca tataacacac tcactaggta catgtag 37 

What is claimed is:
 1. An isolated flea cDNA or flea RNA molecule selected from the group consisting of (a) a flea cDNA or a flea RNA that encodes a protein having an amino acid sequence selected from the group consisting of SEQ ID NO:2 and variants thereof that are at least 95% identical to SEQ ID NO:2 and have peritropin activity; and (b) a flea cDNA or a flea RNA comprising a nucleic acid sequence fully complementary to a nucleic acid molecule of (a).
 2. The nucleic acid molecule of claim 1, wherein said nucleic acid molecule is selected from the group consisting of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, and SEQ ID NO:5.
 3. The nucleic acid molecule of claim 1, wherein said nucleic acid molecule encodes a protein having an amino acid sequence SEQ ID NO:2.
 4. A recombinant molecule comprising a nucleic acid molecule as set forth in claim 1 operatively linked to a transcription control sequence.
 5. A recombinant virus comprising a nucleic acid molecule as set forth in claim
 1. 6. A recombinant cell comprising a nucleic acid molecule as set forth in claim
 1. 7. A composition comprising an isolated nucleic acid molecule of claim 1 and a component selected from the group consisting of an excipient and a carrier.
 8. A method to produce a protein encoded by an isolated nucleic acid molecule of claim 1, said method comprising culturing a cell transformed with a nucleic acid molecule encoding said protein.
 9. The method of claim 8, wherein said protein has an amino acid sequence SEQ ID NO:2.
 10. The method of claim 8, wherein said nucleic acid molecule comprises a nucleic acid sequence selected from the group consisting of: SEQ ID NO:1 and SEQ ID NO:4.
 11. An isolated nucleic acid molecule having a nucleic acid sequence selected from the group consisting of: SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, and SEQ ID NO:5. 